cDNA Clone SPAK

• Synonyms: STK39, DCHT, PASK
• Plasmid: pGEX HA SPAK opt L502A
• Parent Plasmid: pGEX6P1
• Expressed: GST-3C-HA-SPAK L502A
• Genbank: NP_037365.2
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGTACCCATACGATGTGCCAGATTACGCCGCCGAGCCTAGCGGATCTCCCGTGCATGTGCAGCTGCCTCAGCAGGCCGCTCCTGTGACAGCTGCCGCTGCTGCTGCTCCAGCTGCTGCAACAGCAGCTCCTGCTCCTGCAGCACCAGCTGCCCCAGCTCCAGCCCCTGCCCCTGCACCAGCAGCACAGGCTGTGGGATGGCCCATCTGCAGAGATGCCTACGAGCTGCAGGAAGTGATCGGCAGCGGAGCCACAGCAGTGGTGCAGGCTGCCCTGTGCAAGCCCAGACAGGAACGGGTGGCCATCAAGCGGATCAACCTGGAAAAGTGCCAGACCAGCATGGACGAGCTGCTGAAAGAAATCCAGGCCATGAGCCAGTGCAGCCACCCCAACGTCGTGACCTACTACACCAGCTTCGTCGTGAAGGATGAGCTGTGGCTCGTGATGAAGCTGCTGAGCGGCGGCTCTATGCTGGACATCATCAAGTACATCGTGAACCGGGGCGAGCACAAGAACGGCGTGCTGGAAGAGGCCATCATTGCCACCATCCTGAAAGAGGTGCTGGAAGGCCTGGACTACCTGCACCGGAACGGCCAGATCCACAGGGATCTGAAGGCCGGCAACATCCTGCTGGGCGAGGATGGCTCTGTGCAGATCGCCGATTTTGGCGTGTCCGCCTTCCTGGCCACTGGCGGCGACGTGACCAGAAACAAAGTGCGCAAGACCTTCGTGGGCACCCCCTGCTGGATGGCCCCCGAAGTGATGGAACAAGTGCGGGGCTACGACTTCAAGGCCGACATGTGGTCCTTCGGCATCACCGCCATCGAGCTGGCTACAGGCGCCGCTCCCTACCACAAGTACCCCCCCATGAAGGTGCTGATGCTGACCCTGCAGAACGACCCCCCCACCCTGGAAACCGGCGTGGAGGACAAAGAAATGATGAAGAAGTACGGCAAGAGCTTCCGCAAGCTGCTGTCCCTGTGCCTGCAGAAAGATCCCAGCAAGAGGCCCACAGCCGCCGAACTGCTGAAGTGCAAGTTCTTCCAGAAGGCCAAGAACCGCGAGTACCTGATCGAGAAACTGCTGACCCGGACCCCCGATATCGCCCAGCGGGCTAAGAAAGTGCGGAGAGTGCCTGGCAGCTCCGGACATCTGCACAAGACCGAGGACGGCGACTGGGAGTGGAGCGACGACGAGATGGACGAGAAGTCCGAGGAAGGCAAGGCCGCCTTCAGCCAGGAAAAGTCTCGGAGAGTGAAAGAGGAAAACCCCGAGATCGCCGTGTCTGCCAGCACCATCCCTGAGCAGATCCAGAGCCTGAGCGTGCACGATAGCCAGGGCCCTCCCAACGCCAACGAGGACTATAGAGAGGCCAGCAGCTGCGCCGTGAACCTGGTGCTGAGACTGCGGAACAGCCGGAAAGAGCTGAACGACATCAGATTCGAGTTCACCCCCGGCAGAGACACCGCTGATGGGGTGTCCCAGGAACTGTTCAGCGCCGGACTGGTGGACGGCCACGATGTCGTGATTGCGGCCGCCAATCTGCAGAAAATCGTGGACGACCCCAAGGCCCTGAAAACCCTGACCTTCAAGCTGGCCTCCGGCTGCGACGGCAGCGAGATCCCTGATGAAGTGAAGCTGATCGGCTTCGCCCAGCTGAGCGTGTCCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMYPYDVPDYAAEPSGSPVH
VQLPQQAAPVTAAAAAAPAAATAAPAPAAPAAPAPAPAPAPAAQAVGWPI
CRDAYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLK
EIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGE
HKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQI
ADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWS
FGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGK
SFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLTRTPDIA
QRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAFSQEKSRRV
KEENPEIAVSASTIPEQIQSLSVHDSQGPPNANEDYREASSCAVNLVLRL
RNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIAAANLQKIV
DDPKALKTLTFKLASGCDGSEIPDEVKLIGFAQLSVS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU48757
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Synthesised version, nucleotide sequence varies significantly from the wild-type with a large number of silent changes to improve stability. May be grown at 37oC without rearranging.