cDNA Clone FAM83G

• Plasmid: pcDNA-frt-to-c-GFP FAM83G A255H
• Parent Plasmid: pcDNA5D FRT/TO C-GFP
• Expressed: FAM83 A255H-GFP
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccATGGCCTTCTCTCAGGTGCAGTGTCTGGACGACAACCATGTGAA
CTGGCGCTCCAGCGAGTCCAAGCCTGAGTTCTTCTACAGCGAGGAGCAGC
GGCTGGCGCTGGAGGCCCTGGTGGCCCGCGGCCGGGACGCCTTCTACGAG
GTGCTCAAGCGGGAGAACATCCGAGACTTCCTCTCGGAGCTGGAGCTCAA
GCGCATCCTGGAGACCATCGAGGTGTACGACCCGGGCTCTGAGGACCCTC
GGGGCACGGGCCCCTCTCAGGGGCCCGAGGACAATGGGGTCGGCGACGGC
GAGGAAGCCAGCGGGGCGGATGGGGTCCCCATCGAGGCCGAGCCGCTGCC
CTCCCTGGAGTACTGGCCCCAGAAGTCGGACCGCTCCATCCCGCAGCTGG
ACCTGGGCTGGCCCGACACCATCGCCTACCGCGGCGTGACCCGGGCTAGC
GTCTACATGCAGCCCCCCATAGACGGGCAGGCCCACATCAAAGAGGTGGT
GCGGAAGATGATCAGCCAGGCACAGAAGGTGATAGCTGTGGTCATGGACA
TGTTCACCGACGTGGACATCTTCAAGGACCTGCTGGACGCCGGCTTCAAG
AGGAAAGTGGCCGTGTACATCATCGTGGATGAGAGTAACGTCAAGTACTT
CCTGCACATGTGTGAGCGGGCCTGCATGCACCTGGGGCACCTCAAGAATC
TCAGAGTGCGGAGCAGCGGGGGAACTGAGTTCTTCACGCGGTCGGCAACC
AAGTTCAAGGGTGCCCTGcatCAGAAGTTCATGTTTGTGGATGGAGACCG
GGCTGTGTGCGGCTCCTACAGCTTCACGTGGTCGGCCGCGCGGACGGACC
GGAATGTGATCTCTGTGCTGTCTGGCCAGGTGGTGGAGATGTTTGACCGG
CAGTTCCAGGAGCTGTACCTCATGTCACACAGTGTGAGCCTCAAGGGCAT
CCCTATGGAGAAGGAACCGGAGCCGGAGCCTATTGTGCTGCCCTCTGTGG
TCCCCCTGGTGCCCGCGGGCACTGTGGCCAAGAAGCTCGTCAACCCCAAG
TACGCACTTGTCAAGGCCAAGAGCGTCGACGAGATTGCCAAGATCTCCTC
TGAGAAGCAGGAGGCCAAGAAGCCCCTGGGGCTGAAAGGCCCAGCGCTGG
CTGAGCATCCAGGGGAACTCCCCGAGCTGCTGCCACCCATCCACCCAGGA
CTGCTTCACCTGGAGAGGGCCAACATGTTTGAGTACCTGCCCACGTGGGT
GGAGCCAGACCCGGAGCCTGGCAGCGACATCCTGGGCTACATCAATATCA
TCGACCCCAACATCTGGAACCCCCAGCCCAGCCAGATGAACCGCATCAAG
ATCCGTGACACCTCCCAGGCCAGCGCCCAGCACCAGCTGTGGAAGCAGAG
CCAGGACAGCAGGCCCCGTCCAGAGCCTTGCCCTCCCCCAGAGCCCAGTG
CCCCCCAGGACGGTGTCCCAGCTGAGAACGGCCTCCCCCAGGGGGACCCT
GAGCCATTGCCCCCCGTGCCCAAGCCCCGGACAGTCCCTGTGGCAGATGT
ACTAGCCCGGGACAGCAGTGATATTGGCTGGGTCCTGGAGCTCCCCAAAG
AGGAAGCTCCCCAGAATGGGACAGACCATAGGCTACCCAGGATGGCAGGC
CCAGGCCACGCCCCACTCCAGCGGCAGCTATCTGTGACCCAGGATGACCC
CGAGAGCCTCGGGGTGGGGCTCCCCAATGGGCTGGATGGGGTGGAAGAAG
AAGATGATGACGACTACGTAACCCTCAGTGACCAGGACAGCCACTCAGGC
AGCTCCGGCCGTGGCCCTGGCCCCCGACGGCCCTCAGTGGCTTCCTCTGT
GTCAGAGGAGTACTTCGAGGTGAGAGAGCACTCAGTCCCTCTCCGGAGGC
GCCACTCAGAGCAAGTGGCCAACGGGCCAACCCCACCACCGCGCCGGCAG
CTGAGTGCCCCCCATATAACCCGAGGGACCTTTGTTGGACCCCAGGGTGG
CTCCCCATGGGCCCAGAGTCGGGGAAGAGAAGAAGCAGATGCGTTGAAGA
GGATGCAGGCCCAGCGCTCCACAGACAAGGAGGCACAGGGCCAGCAGTTT
CATCATCACAGGGTCCCTGCCTCAGGGACTAGGGATAAAGACGGCTTCCC
AGGACCCCCTAGGTACCGCTCTGCTGCTGACAGCGTCCAGAGCTCTACCA
GAAACGCTGGCCCAGCCATGGCTGGCCCCCACCACTGGCAGGCCAAGGGA
GGTCAGGTACCCCGCCTGCTTCCGGACCCCGGCAGCCCAAGACTGGCCCA
AAATGCCCGCCCCATGACCGATGGCAGGGCCACCGAGGAGCATCCGAGTC
CCTTCGGAATCCCATACTCCAAACTGTCTCAGTCGAAGCACCTAAAGGCC
AGGACGGGCGGTAGCCAGTGGGCCTCATCGGATTCTAAACGGAGGGCTCA
AGCCCCCCGGGACCGCAAAGACCCCgcggccgc

Amino Acid Sequence

MAFSQVQCLDDNHVNWRSSESKPEFFYSEEQRLALEALVARGRDAFYEVL
KRENIRDFLSELELKRILETIEVYDPGSEDPRGTGPSQGPEDNGVGDGEE
ASGADGVPIEAEPLPSLEYWPQKSDRSIPQLDLGWPDTIAYRGVTRASVY
MQPPIDGQAHIKEVVRKMISQAQKVIAVVMDMFTDVDIFKDLLDAGFKRK
VAVYIIVDESNVKYFLHMCERACMHLGHLKNLRVRSSGGTEFFTRSATKF
KGALHQKFMFVDGDRAVCGSYSFTWSAARTDRNVISVLSGQVVEMFDRQF
QELYLMSHSVSLKGIPMEKEPEPEPIVLPSVVPLVPAGTVAKKLVNPKYA
LVKAKSVDEIAKISSEKQEAKKPLGLKGPALAEHPGELPELLPPIHPGLL
HLERANMFEYLPTWVEPDPEPGSDILGYINIIDPNIWNPQPSQMNRIKIR
DTSQASAQHQLWKQSQDSRPRPEPCPPPEPSAPQDGVPAENGLPQGDPEP
LPPVPKPRTVPVADVLARDSSDIGWVLELPKEEAPQNGTDHRLPRMAGPG
HAPLQRQLSVTQDDPESLGVGLPNGLDGVEEEDDDDYVTLSDQDSHSGSS
GRGPGPRRPSVASSVSEEYFEVREHSVPLRRRHSEQVANGPTPPPRRQLS
APHITRGTFVGPQGGSPWAQSRGREEADALKRMQAQRSTDKEAQGQQFHH
HRVPASGTRDKDGFPGPPRYRSAADSVQSSTRNAGPAMAGPHHWQAKGGQ
VPRLLPDPGSPRLAQNARPMTDGRATEEHPSPFGIPYSKLSQSKHLKART
GGSQWASSDSKRRAQAPRDRKDPAAAMVSKGEELFTGVVPILVELDGDVN
GHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSR
YPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRI
ELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
VTAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24671
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: has silent change at nt2271 (T>C) - kills internal BamHI site