cDNA Clone human SV2B

• Synonyms: HsT19680
• Plasmid: pcDNA5D frtTO FLAG SV2B S33A
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: FLAG-SV2B S33A
• Genbank: NM_014848.4
• Tag: FLAG
• Species: Human

Sequence of Insert

GCGGCCGCGATGGATGACTACAAGTATCAGGACAATTATGGGGGCTATGCTCCCAGTGATGGCTATTACCGCGGCAATGAGTCCAACCCAGAAGAAGATGCACAGGCTGATGTCACCGAAGGCCATGATGAGGAAGACGAGATCTATGAGGGCGAGTACCAGGGTATCCCTCACCCAGATGATGTCAAGGCCAAGCAGGCCAAGATGGCGCCCTCCAGAATGGACAGCCTTCGGGGCCAGACAGACCTGATGGCTGAGAGGCTGGAAGATGAGGAGCAGTTGGCCCACCAGTACGAGACCATCATGGATGAGTGTGGCCATGGCCGCTTCCAGTGGATCCTCTTTTTCGTCTTGGGTTTGGCCCTGATGGCCGATGGGGTGGAAGTGTTCGTGGTGAGTTTTGCCCTGCCCAGTGCAGAGAAGGACATGTGTCTGTCCAGCTCCAAAAAAGGAATGCTAGGGATGATAGTCTACTTGGGAATGATGGCGGGCGCCTTCATCCTGGGAGGCCTGGCTGATAAGCTGGGAAGGAAGCGAGTCCTCAGCATGTCTCTGGCCGTCAATGCCTCCTTCGCCTCCCTCTCTTCCTTCGTGCAGGGATATGGAGCCTTCCTCTTCTGCCGACTCATCTCAGGCATCGGTATTGGGGGTGCTCTACCGATTGTTTTTGCCTATTTTTCTGAATTCTTGTCTCGGGAGAAGCGAGGAGAACACCTCAGTTGGCTGGGCATCTTCTGGATGACTGGGGGCCTGTACGCATCTGCCATGGCCTGGAGCATCATCCCACACTATGGCTGGGGCTTCAGCATGGGGACCAATTACCACTTCCATAGCTGGAGAGTGTTTGTCATCGTCTGTGCTCTGCCCTGCACCGTGTCCATGGTGGCCCTGAAGTTCATGCCAGAGAGCCCAAGGTTTCTGCTAGAGATGGGCAAACATGATGAAGCCTGGATGATTCTCAAGCAAGTCCATGACACCAACATGAGAGCTAAGGGGACCCCAGAGAAAGTGTTCACGGTTTCCAACATCAAAACTCCCAAGCAAATGGATGAATTCATTGAGATCCAAAGTTCAACAGGAACCTGGTACCAGCGCTGGCTGGTCAGATTCAAGACCATTTTCAAGCAGGTCTGGGATAATGCCCTGTACTGTGTGATGGGGCCCTACAGAATGAATACACTGATTCTGGCCGTGGTTTGGTTTGCCATGGCATTCAGTTACTATGGACTGACAGTTTGGTTTCCTGATATGATCCGCTATTTTCAAGATGAAGAATACAAGTCTAAAATGAAGGTGTTTTTTGGTGAGCATGTGTACGGCGCCACAATCAACTTCACGATGGAAAATCAGATCCACCAACATGGGAAACTTGTGAATGATAAGTTCACAAGAATGTACTTTAAACATGTACTCTTTGAGGACACATTCTTTGACGAGTGCTATTTTGAAGACGTAACATCAACAGATACCTACTTCAAAAATTGTACCATTGAATCAACCATCTTTTACAACACAGACCTCTACGAGCACAAGTTCATCAACTGTCGGTTTATCAACTCCACCTTCCTGGAGCAGAAGGAGGGCTGCCACATGGACTTGGAGCAAGATAATGACTTCCTGATTTACCTCGTCAGCTTCCTGGGCAGCCTGTCTGTCTTACCCGGGAACATCATTTCTGCCCTGCTCATGGATAGAATTGGAAGGCTCAAGATGATTGGTGGCTCCATGCTAATCTCTGCAGTCTGCTGCTTCTTCCTGTTTTTTGGCAACAGTGAGTCTGCAATGATCGGCTGGCAGTGCCTGTTCTGTGGGACAAGCATTGCAGCCTGGAATGCTCTGGATGTGATCACAGTGGAGCTGTATCCCACCAACCAGAGAGCAACAGCCTTCGGCATTCTCAATGGATTATGCAAATTTGGCGCCATCCTGGGAAACACCATCTTTGCTTCTTTTGTTGGGATAACCAAAGTGGTCCCCATCCTTCTGGCTGCTGCTTCTCTGGTTGGGGGTGGCCTGATTGCCCTTCGACTGCCAGAGACTCGAGAACAGGTCCTGATTTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMDDYKYQDNYGGYAPSDGYYRGNESNPE
EDAQADVTEGHDEEDEIYEGEYQGIPHPDDVKAKQAKMAPSRMDSLRGQT
DLMAERLEDEEQLAHQYETIMDECGHGRFQWILFFVLGLALMADGVEVFV
VSFALPSAEKDMCLSSSKKGMLGMIVYLGMMAGAFILGGLADKLGRKRVL
SMSLAVNASFASLSSFVQGYGAFLFCRLISGIGIGGALPIVFAYFSEFLS
REKRGEHLSWLGIFWMTGGLYASAMAWSIIPHYGWGFSMGTNYHFHSWRV
FVIVCALPCTVSMVALKFMPESPRFLLEMGKHDEAWMILKQVHDTNMRAK
GTPEKVFTVSNIKTPKQMDEFIEIQSSTGTWYQRWLVRFKTIFKQVWDNA
LYCVMGPYRMNTLILAVVWFAMAFSYYGLTVWFPDMIRYFQDEEYKSKMK
VFFGEHVYGATINFTMENQIHQHGKLVNDKFTRMYFKHVLFEDTFFDECY
FEDVTSTDTYFKNCTIESTIFYNTDLYEHKFINCRFINSTFLEQKEGCHM
DLEQDNDFLIYLVSFLGSLSVLPGNIISALLMDRIGRLKMIGGSMLISAV
CCFFLFFGNSESAMIGWQCLFCGTSIAAWNALDVITVELYPTNQRATAFG
ILNGLCKFGAILGNTIFASFVGITKVVPILLAAASLVGGGLIALRLPETR
EQVLI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48583
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: S33A. Also contains an M683/I variation (last amino acid) this is identical to the full-length clones currently in use and a silent T>C change at 432 bp.