cDNA Clone SGK3

• Synonyms: CISK, SGKL
• Plasmid: pSCB SGK3 Nter donor Sal Xho
• Parent Plasmid: pSC-B
• Genbank: ENSG00000104205
• Species: Human

Sequence of Insert

GTCGACTAGTTATGTACTATACAGGATTGTAATCTGAATTGTTCAGCACACTTGACCAGTTCATCCTGATACAAGAAGTCTGAAAACCACACTTTGGTTACCTCCAACTAGATCTTTTCTAGGTGAGAGTTAGTCCCTAGCCTACGTGAACTGAGGTGTGTAAGTTCCTAGAATTTCCTAATAGTAAGAAAGGTAGATGGGCTGCAGATATTTGTGGCTTGTAGTAGGAACATAACAAACTAAATCTCTAACCTGGGCTGAGACTGATGAGCAAAAGTAGGTTCCTATCAGAATAAACGAAAATTAGTCCCATGCTTAAAGAATGACAGTCATAGTATTTTAAAGTTGTTTTGCTAATCACTTTTTTTTTTTTCTGTTGGTTAAGGTTGCATGATGGAATTTGAACATTACTTCAAGAGGTTTTGTATTTTGGATTAGTTAATTGGGTTTGTCCTCTGCTGACTGTTTCTTCGGATGCATTTTTTGGTGTGCTCTTGAGGGATTAAGCCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCAAAGGGACCATACTATGGATTACAAGGAAAGCTGCCCAAGTGTAAGCATTCCCAGCTCCGATGAACACAGAGAGAAAAAGAAGAGGTTTACTGTAAGTATTTAACATAAAGCATGTGGGAAAAAAGTTGAATGTAGAGAATATTTTCATTTCTGCTTTTCTTCTCCAACCTAGAACACCCCCTTCCCCATCTCCACATACCTAGATTTTACTTCTTTCACATGATCTGTTCTCCACAAAGTCTTCTGTGGCTACCGTAATGGAATAAATTTTCCTTTTTTAGTGTCTCTTTACTTATCTTCTTGGAGAATTGTATGAAACTATCTAATTAATCTTCTTAACAGAAAATGTGCATGTGCATACACATGGATCTATGCACACACTTATAATTTTGTGTGCACTGTCAGGAATTAATGGACTCCTGAAGTCATCGATTCCATATTAAGAACTACTGTCCTGAAATGTTTATAGTATCTCTCAGTTTTAAAATATTTAATTCAGTATTTTATTTTTTTCACCTTAAAGGCTCTCGAG

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU48624
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Designed to insert an in-frame GFP downstream of the SGK3 start codon in exon 2. Complete sequence of donor including homology arms shown as sequence of insert, contains several silent mutations to prevent Cas9 targeting the donor sequence. Successful recombination will knockout the SGK3 allele. Contains a BGH polyadenylation signal downstream of GFP to prevent degradation of the transcript via NMD.