cDNA Clone Cullin4b (Xenopus Laevis)

• Plasmid: pBluescript KS+ Dac Tev Cullin4b 1-502 V461R L465D (yeast optimised)
• Parent Plasmid: pBluescript KS+
• Expressed: Dac Tev Cullin4b 1-502 V461R L465D (yeast optimised)
• Tag: DAC
• Species: Xenopus laevis

Sequence of Insert

ATGAAGGACTTGGTTGCTTCCCCATCTGGTCAATCTAAGCAAGGTTTGTCTTCCCCAAACCCACCAGCTCAAGAAGTTAGAACCGAAGGTAACTCTACCTCTAAGAAGAGAAGATTGAACATCTCTTCTTCCGACAAGGATGACTTGGATCCATTGTCCCCAGCTAGAAACTCTTCCTCCTCTTCTAACCACATTCAAAAGAAGTTGAGATTCGAAGACACTTTGGACTTCTTGGGTTTGGATGTCAAGATGACTGAAGAATCTTCTTCTTCTTCTTCCTGTTCTTTCTCTTCTACCCCACAAGCTGTTAGATCCAAGAACTTGTTGATTTCTCCAGTTGGTGTTGGTCACCACGCTAACGGTTTGACTAAGGCTTCTCCAGTTTCTTCTTTCGCTAACTCCAAGCCAGGTTCTGCTAAGAAGTTGGTCATTAAGAACTTCAAGGATAAGCCAAAGTTGCCAGAAAACTACACCGACGAAACTTGGCAAAAGTTGAAGGGTACCGTTGAAGCTATCCAAAACTCCACTTCCATTAAGTACAACTTGGAAGAATTGTACCAAGCTGTCGAAAACTTGTGTTCTTACAAGATCTCCGCTAACTTGTACAAGCAATTGAGACAAATTTGTGAAGATCACATTAAGGCTCAAATTCACCAATTCAGAGAAGACTCTTTGGATTCTGTTTTGTTCTTGAAGAAGATTGACAAGTGTTGGAAGGACCACTGTAGACAAATGATTATGATTAGATCTATCTTCTTGTTCTTGGATAGAACTTACGTTTTGCAAAACTCTATGTTGCCATCTATTTGGGACATGGGTTTGGAATTGTTCAGAACTCACATCATTTCCGACCAAAAGGTCCAAAACAAGACTATTGACGGTATCTTGTTGTTGATCGAAAGAGAAAGAAACGGTGAAGCTATTGATAGATCTTTGTTGAGATCCTTGTTGTCCATGTTGTCTGACTTGCAAATCTACCAAGACTCCTTCGAACAAAGATTCTTGGAAGAAACTAACAGATTGTACTCTGCTGAAGGTCAAAGATTGATGCAAGAAAGAGAAGTCCCAGAATACTTGCACCACGTCAACAAGAGATTGGAAGAAGAAGCTGACAGAGTTATTACTTACTTGGATCAATCCACTCAAAAGCCATTGATCGCTACTGTTGAAAAGCAATTGTTGGGTGAACACTTGACCGCTACTTTGCAAAAGGGTTTGAACCACTTGTTGGACGAAAACAGAATCCAAGATTTGTCCTTGTTGTACCAATTGTTCTCTAGAGTCAGAGGTGGTGTTCAAGTCTTGTTGCAACACTGGATTGAATACATTAAGGCTTTCGGTTCTACCATCGTCATCAACCCAGAAAAGGATAAGACTATGAGACAAGAATTGGACGACTTCAAGGACAAGGTTGACCACGTTATTGACGTTTGTTTCTTGAAGAACGAAAAGTTCGTTAACGCTATGAAGGAAGCTTTCGAAACTTTCATCAACAAGAGACCAAACTAAGG

Amino Acid Sequence

MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKLLTWGFRFFETVNPENLYFQGGSMKDLVASPSGQSKQGLSSPNPPAQEVRTEGNSTSKKRRLNISSSDKDDLDPLSPARNSSSSSNHIQKKLRFEDTLDFLGLDVKMTEESSSSSSCSFSSTPQAVRSKNLLISPVGVGHHANGLTKASPVSSFANSKPGSAKKLVIKNFKDKPKLPENYTDETWQKLKGTVEAIQNSTSIKYNLEELYQAVENLCSYKISANLYKQLRQICEDHIKAQIHQFREDSLDSVLFLKKIDKCWKDHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRTHIISDQKVQNKTIDGILLLIERERNGEAIDRSLLRSLLSMLSDLQIYQDSFEQRFLEETNRLYSAEGQRLMQEREVPEYLHHVNKRLEEEADRVITYLDQSTQKPLIATVEKQLLGEHLTATLQKGLNHLLDENRIQDLSLLYQLFSRVRGGVQVLLQHWIEYIKAFGSTIVINPEKDKTMRQELDDFKDKVDHVIDVCFLKNEKFVNAMKEAFETFINKRPN*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU25984
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Clone given by Karim Labib Group