cDNA Clone SGK3

• Synonyms: CISK, SGKL, SGK2
• Plasmid: pU6D shuttle SGK3 Common KO sense B
• Parent Plasmid: pU6D shuttle
• Expressed: SGK3 Common KO sense B
• Genbank: ENSG00000104205
• Species: Human

Sequence of Insert

GTCAGATCCATCTGAAGATG

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU48709
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR guide plasmid. Targets sense strand in first common coding exon, may be used in conjunction with 48710 and Cas9 D10A where possible to improve specificity. Western blotting should be preferentially used to screen for KOs as there is a good antibody. The insert may be excised and shuttled to destination lentiviral vectors as necessary.