cDNA Clone Ubiquitin

• Plasmid: pET28a DAC TEV-Ubiquitin dimer
• Parent Plasmid: pET28a DAC TEV
• Expressed: DAC-Ubiquitin dimer
• Genbank: P62988
• Tag: DAC
• Species: Human

Sequence of Insert

GGATCCGCCGGCATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCAT
CACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGA
TCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCT
GGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAA
AGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGCGGCATGCAGATCT
TCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGT
GACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCC
TCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGAC
GCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTC
CTCCGTCTCAGAGGCGGCTGATAAGCGGCCGC

Amino Acid Sequence

MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIG
QAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRG
INLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDA
DGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDN
SLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKL
LTWGFRFFETVNPENLYFQGGSAGMQIFVKTLTGKTITLEVEPSDTIENV
KAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLRGG**

• Vector Type: Bacterial
• Antibiotic: Kan
• Full Snapgene Map: pET28a DAC TEV-Ubiquitin dimer46.9 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU24942
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 21852
• Low Temp: No