cDNA Clone SPAK

• Plasmid: pet15 SPAK kdFL 1-547 D212A
• Parent Plasmid: pET15
• Expressed: 6*HIS- SPAK kdFL (1-547, D212A)
• Genbank: AF099989
• Tag: 6His

Sequence of Insert

GGATCCATGGCCGAGCCTAGCGGATCTCCCGTGCATGTGCAGCTGCCTCAGCAGGCCGCTCCTGTGACAGCTGCCGCTGCTGCTGCTCCAGCTGCTGCAACAGCAGCTCCTGCTCCTGCAGCACCAGCTGCCCCAGCTCCAGCCCCTGCCCCTGCACCAGCAGCACAGGCTGTGGGATGGCCCATCTGCAGAGATGCCTACGAGCTGCAGGAAGTGATCGGCAGCGGAGCCACAGCAGTGGTGCAGGCTGCCCTGTGCAAGCCCAGACAGGAACGGGTGGCCATCAAGCGGATCAACCTGGAAAAGTGCCAGACCAGCATGGACGAGCTGCTGAAAGAAATCCAGGCCATGAGCCAGTGCAGCCACCCCAACGTCGTGACCTACTACACCAGCTTCGTCGTGAAGGATGAGCTGTGGCTCGTGATGAAGCTGCTGAGCGGCGGCTCTATGCTGGACATCATCAAGTACATCGTGAACCGGGGCGAGCACAAGAACGGCGTGCTGGAAGAGGCCATCATTGCCACCATCCTGAAAGAGGTGCTGGAAGGCCTGGACTACCTGCACCGGAACGGCCAGATCCACAGGGATCTGAAGGCCGGCAACATCCTGCTGGGCGAGGATGGCTCTGTGCAGATCGCCGCTTTTGGCGTGTCCGCCTTCCTGGCCACTGGCGGCGACGTGACCAGAAACAAAGTGCGCAAGACCTTCGTGGGCACCCCCTGCTGGATGGCCCCCGAAGTGATGGAACAAGTGCGGGGCTACGACTTCAAGGCCGACATGTGGTCCTTCGGCATCACCGCCATCGAGCTGGCTACAGGCGCCGCTCCCTACCACAAGTACCCCCCCATGAAGGTGCTGATGCTGACCCTGCAGAACGACCCCCCCACCCTGGAAACCGGCGTGGAGGACAAAGAAATGATGAAGAAGTACGGCAAGAGCTTCCGCAAGCTGCTGTCCCTGTGCCTGCAGAAAGATCCCAGCAAGAGGCCCACAGCCGCCGAACTGCTGAAGTGCAAGTTCTTCCAGAAGGCCAAGAACCGCGAGTACCTGATCGAGAAACTGCTGACCCGGACCCCCGATATCGCCCAGCGGGCTAAGAAAGTGCGGAGAGTGCCTGGCAGCTCCGGACATCTGCACAAGACCGAGGACGGCGACTGGGAGTGGAGCGACGACGAGATGGACGAGAAGTCCGAGGAAGGCAAGGCCGCCTTCAGCCAGGAAAAGTCTCGGAGAGTGAAAGAGGAAAACCCCGAGATCGCCGTGTCTGCCAGCACCATCCCTGAGCAGATCCAGAGCCTGAGCGTGCACGATAGCCAGGGCCCTCCCAACGCCAACGAGGACTATAGAGAGGCCAGCAGCTGCGCCGTGAACCTGGTGCTGAGACTGCGGAACAGCCGGAAAGAGCTGAACGACATCAGATTCGAGTTCACCCCCGGCAGAGACACCGCTGATGGGGTGTCCCAGGAACTGTTCAGCGCCGGACTGGTGGACGGCCACGATGTCGTGATTGTGGCCGCCAATCTGCAGAAAATCGTGGACGACCCCAAGGCCCTGAAAACCCTGACCTTCAAGCTGGCCTCCGGCTGCGACGGCAGCGAGATCCCTGATGAAGTGAAGCTGATCGGCTTCGCCCAGCTGAGCGTGTCCTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMAEPSGSPVHVQLPQQAAPVTAAAAAAPAAATAAPAPAAPAAPAPAPAPAPAAQAVGWPICRDAYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIAAFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAFSQEKSRRVKEENPEIAVSASTIPEQIQSLSVHDSQGPPNANEDYREASSCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGSEIPDEVKLIGFAQLSVS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU55156
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please put from GST (DU6013) into both 6*HIS (only) and 6*HIS-SUMO bacterial expression vector.