cDNA Clone P70 (rat)

• Synonyms: Rps6kb1
• Plasmid: pCMV5 HA p70 S6 kinase M1-S421 T412E
• Parent Plasmid: pCMV5
• Expressed: HA p70 S6 kinase M1-S421 T412E
• Genbank: NM_031985.1 GI:14010888 with several silent changes and R367P (common variant)
• Tag: HA
• Species: Rat

Sequence of Insert

GCGGCCGCCACCATGTACCCTTACGATGTTCCAGATTACGCTGGGCCCATGAGGCGACGACGGAGGCGGGACGGCTTTTACCCAGCGCCTGACTTCCGACACAGGGAAGCTGAGGACATGGCAGGAGTGTTTGACATAGACCTGGACCAGCCAGAGGATGCAGGCTCTGAGGATGAGCTGGAGGAGGGGGGTCAGTTAAATGAAAGCATGGACCATGGGGGAGTTGGACCATATGAACTTGGCATGGAACATTGTGAGAAATTTGAAATCTCAGAAACTAGTGTGAACAGAGGGCCAGAAAAAATCAGACCAGAATGTTTTGAGCTACTTCGGGTACTTGGTAAAGGGGGCTATGGAAAGGTTTTTCAAGTACGAAAAGTAACAGGAGCAAATACTGGGAAGATATTTGCCATGAAGGTGCTTAAAAAGGCAATGATAGTAAGAAATGCTAAAGATACAGCTCATACAAAAGCAGAGCGGAATATTCTGGAGGAAGTAAAGCATCCCTTCATTGTGGATTTAATTTATGCCTTTCAGACCGGTGGAAAACTCTACCTCATCCTTGAGTATCTCAGTGGAGGAGAACTATTTATGCAGTTAGAAAGAGAGGGGATATTCATGGAAGATACAGCTTGCTTTTACTTGGCTGAAATCTCCATGGCTTTGGGGCATTTACATCAAAAAGGGATCATCTACAGAGACCTGAAGCCGGAGAACATCATGCTTAATCACCAAGGTCACGTGAAGCTGACAGACTTTGGACTATGCAAAGAATCTATTCATGATGGAACAGTCACGCACACATTTTGTGGAACAATAGAATACATGGCCCCTGAAATCTTGATGAGAAGCGGCCACAACCGTGCTGTGGATTGGTGGAGTTTGGGAGCATTAATGTATGACATGCTGACTGGAGCACCTCCATTCACTGGGGAGAATAGAAAGAAAACAATTGACAAAATCCTCAAATGTAAACTTAATTTGCCTCCCTACCTCACACAAGAAGCTCGAGATCTGCTTAAAAAGCTGCTGAAAAGAAATGCTGCTTCTCGTCTTGGAGCTGGCCCTGGGGATGCTGGAGAAGTCCAAGCGCATCCATTTTTTAGACACATTAACTGGGAAGAGCTTTTGGCTCGGAAGGTGGAGCCCCCCTTTAAGCCTCTGTTGCAATCTGAAGAGGATGTGAGTCAGTTTGATTCAAAGTTTACTCGTCAGACACCTGTTGACAGCCCCGATGACTCAACTCTCAGTGAAAGTGCCAACCAGGTCTTTCTGGGTTTCGAATACGTGGCTCCATCTGTACTTGAAAGTTGATAAGAAAACTTTTCTTTTGAACCAAAAATCCGATCGCCTCGAAGATTTATTGGTAGCCCACGAACGCCTGTCAGCCCAGTCAAATTCTCTCCTGGGGATTTCTGGGGACGAGGTGCTTCAGCCAGCACAGCAAATCCTCAGACACCTGTGGAATACCCAATGGAAACAAGTGGAATAGAGCAGATGGATGTGACAACGAGCGGGGAAGCTTCAGCGCCACTTCCAATCCGACAGCCCAACTCTGGGCCATACAAAAAACAAGCTTTTCCTATGATCTCCAAACGGCCAGAGCACCTGCGTATGAATCTATGATGAAACAATGCTTTTATTAATGCAAATGCAAAAAGGAAACAAATCCTGGAAGGGGATGTGTGAGGATCCTGCAAGATAAAAATGAGAAAATGGCAGTCTCAAAGAGTCAGTGTCATTACCTGGAATGCTTTCGATGGAGGAAAAAATAAACATGGATTTTTAAAAAAATCCCCGGGAATTC

Amino Acid Sequence

MYPYDVPDYAGPMRRRRRRDGFYPAPDFRHREAEDMAGVFDIDLDQPEDAGSEDELEEGGQLNESMDHGGVGPYELGMEHCEKFEISETSVNRGPEKIRP
ECFELLRVLGKGGYGKVFQVRKVTGANTGKIFAMKVLKKAMIVRNAKDTAHTKAERNILEEVKHPFIVDLIYAFQTGGKLYLILEYLSGGELFMQLEREG
IFMEDTACFYLAEISMALGHLHQKGIIYRDLKPENIMLNHQGHVKLTDFGLCKESIHDGTVTHTFCGTIEYMAPEILMRSGHNRAVDWWSLGALMYDMLT
GAPPFTGENRKKTIDKILKCKLNLPPYLTQEARDLLKKLLKRNAASRLGAGPGDAGEVQAHPFFRHINWEELLARKVEPPFKPLLQSEEDVSQFDSKFTR
QTPVDSPDDSTLSESANQVFLGFEYVAPSVLES*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU53345
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: (Inserted sequence contains some 3' UTR)