cDNA Clone TBCK

• Plasmid: pET15b 6HIS SUMO TBCK
• Parent Plasmid: pET15b 6His SUMO
• Expressed: 6HIS SUMO TBCK
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

GGATCCATGTTTCCCCTGAAGGACGCTGAAATGGGAGCCTTTACCTTCTTTGCCTCGGCTCTGCCACATGATGTTTGTGGAAGCAATGGACTTCCTCTCACACCAAATTCCATCAAAATTTTAGGGCGCTTTCAAATCCTTAAAACCATCACCCATCCCAGACTCTGCCAGTATGTGGATATTTCTAGGGGAAAGCATGAACGACTAGTGGTCGTGGCTGAACATTGTGAACGTAGTCTGGAAGACTTGCTTCGAGAAAGGAAACCTGTGAGCTGTTCAACGGTTTTGTGTATAGCATTTGAGGTTCTTCAGGGCTTGCAGTATATGAACAAACATGGTATAGTACACAGGGCATTGTCTCCTCATAATATCCTGTTGGACCGAAAGGGACATATTAAATTGGCTAAATTTGGACTTTATCACATGACAGCTCATGGTGATGATGTTGATTTCCCAATAGGGTATCCCTCGTACTTGGCCCCTGAGGTAATTGCACAGGGAATTTTCAAAACCACTGATCACATGCCAAGTAAAAAACCATTGCCTTCTGGCCCCAAATCAGATGTATGGTCTCTTGGAATCATTTTATTTGAGCTTTGTGTGGGAAGAAAATTATTTCAGAGCTTGGATATTTCTGAAAGACTAAAATTTTTGCTTACTTTGGATTGTGTAGATGACACTTTAATAGTTCTGGCTGAAGAGCATGGTTGTTTGGACATTATAAAGGAGCTTCCTGAAACTGTGATAGATCTTTTGAATAAGTGCCTTACCTTCCATCCTTCTAAGAGGCCAACCCCAGATGAATTAATGAAGGACAAAGTATTCAGTGAGGTATCACCTTTATATACCCCCTTTACCAAACCTGCCAGTCTGTTTTCATCTTCTCTGAGATGTGCTGATTTAACTCTGCCTGAGGATATCAGTCAGTTGTGTAAAGATATAAATAATGATTACCTGGCAGAAAGATCTATTGAAGAAGTGTATTACCTTTGGTGTTTGGCTGGAGGTGACTTGGAGAAAGAGCTTGTCAACAAGGAAATCATTCGATCCAAACCACCTATCTGCACACTCCCCAATTTTCTCTTTGAGGATGGTGAAAGCTTTGGACAAGGTCGAGATAGAAGCTCGCTTTTAGATGATACCACTGTGACATTGTCGTTATGCCAGCTAAGAAATAGATTGAAAGATGTTGGTGGAGAAGCATTTTACCCATTACTTGAAGATGACCAGTCTAATTTACCTCATTCAAACAGCAATAATGAGTTGTCTGCAGCTGCCACGCTCCCTTTAATCATCAGAGAGAAGGATACAGAGTACCAACTAAATAGAATTATTCTCTTCGACAGGCTGCTAAAGGCTTATCCATATAAAAAAAACCAAATCTGGAAAGAAGCAAGAGTTGACATTCCTCCTCTTATGAGAGGTTTAACCTGGGCTGCTCTTCTGGGAGTTGAGGGAGCTATTCATGCCAAGTACGATGCAATTGATAAAGACACTCCAATTCCTACAGATAGACAAATTGAAGTGGATATTCCTCGCTGTCATCAGTACGATGAACTGTTATCATCACCAGAAGGTCATGCAAAATTTAGGCGTGTATTAAAAGCCTGGGTAGTGTCTCATCCTGATCTTGTGTATTGGCAAGGTCTTGACTCACTTTGTGCTCCATTCCTATATCTAAACTTCAATAATGAAGCCTTGGCTTATGCATGTATGTCTGCTTTTATTCCCAAATACCTGTATAACTTCTTCTTAAAAGACAACTCACATGTAATACAAGAGTATCTGACTGTCTTCTCTCAGATGATTGCATTTCATGATCCAGAGCTGAGTAATCATCTCAATGAGATTGGTTTCATTCCAGATCTCTATGCCATCCCTTGGTTTCTTACCATGTTTACTCATGTATTTCCACTACACAAAATTTTCCACCTCTGGGATACCTTACTACTTGGGAATTCCTCTTTCCCATTCTGTATTGGAGTAGCAATTCTTCAGCAGCTGCGGGACCGGCTTTTGGCTAATGGCTTTAATGAGTGTATTCTTCTCTTCTCCGATTTACCAGAAATTGACATTGAACGCTGTGTGAGAGAATCTATCAACCTGTTTTGTTGGACTCCTAAAAGTGCTACTTACAGACAGCATGCTCAACCTCCAAAGCCATCTTCTGACAGCAGTGGAGGCAGAAGTTCGGCACCTTATTTCTCTGCTGAGTGTCCAGATCCTCCAAAGACAGATCTGTCAAGAGAATCCATCCCATTAAATGACCTGAAGTCAGAAGTATCACCACGGATTTCAGCAGAGGACCTGATTGACTTGTGTGAGCTCACAGTGACAGGCCACTTCAAAACACCCAGCAAGAAAACAAAGTCCAGTAAACCAAAGCTCCTGGTGGTTGACATCCGGAATAGTGAAGACTTTATTCGTGGTCACATTTCAGGAAGCATCAACATTCCATTCAGTGCTGCCTTCACTGCAGAAGGGGAGCTTACCCAGGGCCCTTACACTGCTATGCTCCAGAACTTCAAAGGGAAGGTCATTGTCATCGTGGGGCATGTGGCAAAACACACAGCTGAGTTTGCAGCTCACCTTGTGAAGATGAAATATCCAAGAATCTGTATTCTAGATGGTGGCATTAATAAAATAAAGCCAACAGGCCTCCTCACCATCCCATCTCCTCAAATATGAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMFPLKDAEMGAFTFFASALPHDVCGSNGLPLTPNSIKILGRFQILKTITHPRLCQYVDISRGKHERLVVVAEHCERSLEDLLRERKPVSCSTVLCIAFEVLQGLQYMNKHGIVHRALSPHNILLDRKGHIKLAKFGLYHMTAHGDDVDFPIGYPSYLAPEVIAQGIFKTTDHMPSKKPLPSGPKSDVWSLGIILFELCVGRKLFQSLDISERLKFLLTLDCVDDTLIVLAEEHGCLDIIKELPETVIDLLNKCLTFHPSKRPTPDELMKDKVFSEVSPLYTPFTKPASLFSSSLRCADLTLPEDISQLCKDINNDYLAERSIEEVYYLWCLAGGDLEKELVNKEIIRSKPPICTLPNFLFEDGESFGQGRDRSSLLDDTTVTLSLCQLRNRLKDVGGEAFYPLLEDDQSNLPHSNSNNELSAAATLPLIIREKDTEYQLNRIILFDRLLKAYPYKKNQIWKEARVDIPPLMRGLTWAALLGVEGAIHAKYDAIDKDTPIPTDRQIEVDIPRCHQYDELLSSPEGHAKFRRVLKAWVVSHPDLVYWQGLDSLCAPFLYLNFNNEALAYACMSAFIPKYLYNFFLKDNSHVIQEYLTVFSQMIAFHDPELSNHLNEIGFIPDLYAIPWFLTMFTHVFPLHKIFHLWDTLLLGNSSFPFCIGVAILQQLRDRLLANGFNECILLFSDLPEIDIERCVRESINLFCWTPKSATYRQHAQPPKPSSDSSGGRSSAPYFSAECPDPPKTDLSRESIPLNDLKSEVSPRISAEDLIDLCELTVTGHFKTPSKKTKSSKPKLLVVDIRNSEDFIRGHISGSINIPFSAAFTAEGELTQGPYTAMLQNFKGKVIVIVGHVAKHTAEFAAHLVKMKYPRICILDGGINKIKPTGLLTIPSPQI*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU26682
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Linker after SENP cleavage deleted