cDNA Clone preScission Protease

• Plasmid: pGEX4T PreScission Protease
• Parent Plasmid: pGEX4T1
• Expressed: GST-Thrombin-preScission Protease
• Genbank: NP_740524.1
• Tag: GST

Sequence of Insert

GGATCCGGTGGAGGACCAAACACAGAATTTGCACTATCCCTGTTAAGGAAAAACATAATGACTATAACAACCTCAAAGGGAGAGTTCACAGGGTTAGGCATACATGATCGTGTCTGTGTGATACCCACACACGCACAGCCTGGTGATGATGTACTAGTAAATGGTCAGAAAATTAGAGTTAAGGATAAGTACAAATTAGTAGATCCAGAGAACATTAATCTAGAGCTTACAGTGTTGACTTTAGATAGAAATGAAAAATTCAGAGATATCAGGGGATTTATATCAGAAGATCTAGAAGGTGTGGATGCCACTTTGGTAGTACATTCAAATAACTTTACCAACACTATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAGCACCCCCACTAACAGAATGATTCGTTATGATTATGCAACAAAAACTGGGCAGTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTGGCGGTAATGGAAGACAAGGATTTTCAGCTCAACTTAAAAAACAATATTTTGTAGAGAAACAATGAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERA
EISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQID
KYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSGGGPNTEFALSLLRKNIMTITTSKGEFTGLGIHDRVCVIPTHAQPGDDVLVNGQKIRVKDKYKL
VDPENINLELTVLTLDRNEKFRDIRGFISEDLEGVDATLVVHSNNFTNTILEVGPVTMAGLINLSSTPTNRMIRYDYATKTGQCGGVLCATGKIFGIHVG
GNGRQGFSAQLKKQYFVEKQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU2328
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: PreScission Protease from Human rhinovirus B14