cDNA Clone preScission Protease

• Plasmid: pStrepII-Linker-GEX4T PreScission Protease
• Parent Plasmid: pGEX4T1
• Expressed: StrepII-Linker-GST-Thrombin-preScission Protease
• Genbank: NP_740524.1
• Tag: GST

Sequence of Insert

GGATCCGGTGGAGGACCAAACACAGAATTTGCACTATCCCTGTTAAGGAAAAACATAATGACTATAACAACCTCAAAGGGAGAGTTCACAGGGTTAGGCATACATGATCGTGTCTGTGTGATACCCACACACGCACAGCCTGGTGATGATGTACTAGTAAATGGTCAGAAAATTAGAGTTAAGGATAAGTACAAATTAGTAGATCCAGAGAACATTAATCTAGAGCTTACAGTGTTGACTTTAGATAGAAATGAAAAATTCAGAGATATCAGGGGATTTATATCAGAAGATCTAGAAGGTGTGGATGCCACTTTGGTAGTACATTCAAATAACTTTACCAACACTATCTTAGAAGTTGGCCCTGTAACAATGGCAGGACTTATTAATTTGAGTAGCACCCCCACTAACAGAATGATTCGTTATGATTATGCAACAAAAACTGGGCAGTGTGGAGGTGTGCTGTGTGCTACTGGTAAGATCTTTGGTATTCATGTTGGCGGTAATGGAAGACAAGGATTTTCAGCTCAACTTAAAAAACAATATTTTGTAGAGAAACAATGAGAATTC

Amino Acid Sequence

MWSHPQFEKGGGSGGGSGGGMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHN
MLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFK
KRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSGGGPNTEFALSLLRKNIMTITTSKGEFTGLGIHDRVCVIPTHAQPGDDVLVNGQ
KIRVKDKYKLVDPENINLELTVLTLDRNEKFRDIRGFISEDLEGVDATLVVHSNNFTNTILEVGPVTMAGLINLSSTPTNRMIRYDYATKTGQCGGVLCA
TGKIFGIHVGGNGRQGFSAQLKKQYFVEKQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU53396
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: PreScission Protease from Human rhinovirus B14