cDNA Clone Mouse Amphiphysin

• Synonyms: Amph
• Plasmid: pET15 6His 3C mouse Amphiphysin isoform 2
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His 3C mouse Amphiphysin isoform 2
• Genbank: NM_001289546.1 GI:575501702 - See notes
• Tag: 6His
• Species: Mouse

Sequence of Insert

GGATCCATGGCCGACATCAAGACGGGCATCTTCGCCAAAAACGTGCAAAAGCGCCTCAACCGCGCGCAGGAAAAGGTCCTCCAGAAGCTGGGGAAGGCTGACGAGACAAAAGATGAACAGTTTGAAGAATATGTCCAGAACTTCAAACGGCAGGAGGCAGAGGGTACCAGACTTCAGCGAGAACTCCGGGGATATTTAGCAGCAATCAAAGGTATGCAGGAGGCCTCAATGAAGCTCACTGAATCTCTACATGAAGTCTACGAGCCAGACTGGTATGGACGGGAAGATGTAAAGATGGTTGGTGAGAAGTGTGATGTGCTGTGGGAAGACTTCCATCAAAAGCTAGTGGATGGCTCCTTGCTGACACTGGACACCTACCTGGGCCAGTTTCCTGACATAAAGAATCGCATCGCTAAGCGCAGCAGGAAGCTTGTGGATTATGATAGTGCTCGCCATCACCTGGAGGCTCTGCAGAGTTCCAAACGGAAAGATGAGAGTCGGATCTCTAAGGCAGAAGAGGAGTTTCAGAAAGCACAGAAAGTGTTTGAAGAGTTCAATGTTGACTTACAAGAAGAGTTGCCGTCATTGTGGTCAAGTCGAGTTGGCTTTTACGTCAATACTTTCAAAAATGTCTCCAGCCTTGAGGCCAAGTTTCATAAGGAAATCGCAGTGCTTTGCCACAAGCTCTATGAAGTGATGACGAAACTCGGTGATCAGCATGCTGACAAGGCCTTCAGCATTCAAGGAGCTCCCAGTGACTCGGGTCCTCTTCGCATTGCAAAGACACCATCACCTCCAGAGGAGCCTTCTCCCCTGCCCAGCCCCACGGCTAGTCCCAACCACACATTAGCACCTGCATCTCCTGCCCCAGTGCGACCCAGATCACCTTCACAGACAAGGAAAGGGCCTCCTGTCCCACCTCTGCCTAAAGTCACCCCAACAAAGGAACTGAAACAGGAGAACATCATCAATTTCTTTGAGGACAACTTTGTACCAGAAATCAATGTGACAACACCTTCCCAGAACGAAGTCTTGGAGGTGAAGAAGGAAGAGACTTTGCTGGATTTGGACTTTGACCCTTTCAAGCCCGATGTGGCTCCGGCAGGTTCTGCTGCAGCGACCCACTCTCCCATGTCTCAGACATTGCCCTGGGACTTATGGACGACAAGCACTGATTTGGTACAACCAGCTTCTGGGGGTTCATTTAATGATTTCACCCAGGCCCAGGACACTTCATTATTCACCATGCAGACAGATCAGAATATGGTAGGTGGCTTGGCTGAAACTGAGCAGGCTCTACCTACAGAGCCCCAAGCCGAAGAGCCTCCTGCCACTGCTGCTGCACCTACTGCTGGGCTGGACCTTGGGCTAGAGATGGAGGAGCCAAAGGAGGAGGCTGTGATCCCTCCCGCATCTGATACTGGCGAGACTGTGGAAACTGCAGTGCCAACTGAGGGTGCCCCGGTGGAGGAAGCAGAGGCAGAGAAGGCAGCCCTCCCTGCTGGAGAAGGAGGAAGTCCAGAGGGAGCCAAAATTGACGGGGAAAGCACGGAGCTTGCAATCAGTGAGAGCCCTCAACCAGCGGAGCCCGAAGCGGGTGCTCCTCAGGCCATCCCTTCTGTTGTCATTGAGCCAGCCTCCAACCATGAAGGAGAAGGAGAACACCAAGAAACTGCTACGGGTACTGAACCGAGGGAGGCCGCTGAGGATGTGGCTGCCCAGGGCTCAGCGGGTGAGAAGCAGGAAGTGGCTACAGAGCCCACGCCTCTGGATTCTCAGGCAACTATGCCTGCATCTGCAGGTGCTATGGATGCCTCGCTGTCTGCAGGGGATGCCACTCAGGAACTGCCTCCTGGCTTTCTCTACAAGGTGGAAACCCTGCATGATTTCGAGGCAGCGAACTCTGATGAACTTAACCTACAAAGGGGTGACGTGGTACTCGTGGTGCCTTCAGACTCAGAAGCCGACCAGGATGCAGGCTGGTTGGTGGGGGTGAAGGAGTCAGACTGGCTTCAGTACAGAGACCTGGCCACGTACAAAGGCCTCTTTCCAGAGAATTTCACGCGGCGCCTGGAGTAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMADIKTGIFAKNVQKRLNRAQEKVLQKLGKADETKDEQFEEYVQNFKRQEAEGTRLQRELRGYLAAIKGMQEASMKL
TESLHEVYEPDWYGREDVKMVGEKCDVLWEDFHQKLVDGSLLTLDTYLGQFPDIKNRIAKRSRKLVDYDSARHHLEALQSSKRKDESRISKAEEEFQKAQ
KVFEEFNVDLQEELPSLWSSRVGFYVNTFKNVSSLEAKFHKEIAVLCHKLYEVMTKLGDQHADKAFSIQGAPSDSGPLRIAKTPSPPEEPSPLPSPTASP
NHTLAPASPAPVRPRSPSQTRKGPPVPPLPKVTPTKELKQENIINFFEDNFVPEINVTTPSQNEVLEVKKEETLLDLDFDPFKPDVAPAGSAAATHSPMS
QTLPWDLWTTSTDLVQPASGGSFNDFTQAQDTSLFTMQTDQNMVGGLAETEQALPTEPQAEEPPATAAAPTAGLDLGLEMEEPKEEAVIPPASDTGETVE
TAVPTEGAPVEEAEAEKAALPAGEGGSPEGAKIDGESTELAISESPQPAEPEAGAPQAIPSVVIEPASNHEGEGEHQETATGTEPREAAEDVAAQGSAGE
KQEVATEPTPLDSQATMPASAGAMDASLSAGDATQELPPGFLYKVETLHDFEAANSDELNLQRGDVVLVVPSDSEADQDAGWLVGVKESDWLQYRDLATY
KGLFPENFTRRLE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU53442
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: With the following residue changes:; T470S, V526A, V535A, L594M and V601M. Derived from RNA source.