cDNA Clone Shroom4

• Plasmid: pcDNA FRT TO GFP Shroom4 R146W
• Parent Plasmid: pcDNA FRT TO GFP
• Expressed: GFP Shroom4 R146W
• Tag: GFP
• Species: Human

Sequence of Insert

GAATTCATGGAGAACCGGCCTGGGTCCTTCCAGTACGTCCCTGTGCAGCTGCAAGGGGGGGCACCCTGGGGCTTCACCCTTAAGGGGGGTCTGGAACACTGTGAGCCGCTCACAGTGTCTAAGATTGAAGATGGAGGCAAGGCAGCTTTGTCCCAGAAGATGAGGACTGGTGATGAGCTGGTGAATATCAATGGCACTCCATTATATGGCTCCCGCCAAGAGGCCCTCATTCTCATCAAAGGCTCCTTCCGGATTCTCAAGCTGATTGTCAGGAGGAGGAACGCCCCTGTCAGTAGGCCGCACTCATGGCATGTGGCCAAGCTGCTGGAGGGATGCCCTGAAGCAGCCACCACCATGCATTTCCCTTCTGAAGCCTTCAGCTTGTCCTGGCATTCTGGCTGCAACACAAGTGACGTGTGTGTGCAGTGGTGTCCACTCTCCTGGCATTGCAGCACCGAGAAAAGCAGCTCCATTGGCAGCATGGAGAGCCTGGAGCAACCAGGCCAAGCCACCTATGAGAGCCATCTGTTGCCTATTGACCAGAACATGTACCCTAACCAGCGTGACTCAGCCTACAGCTCCTTCTCGGCCAGCTCAAATGCTTCTGACTGTGCCCTTTCCCTCAGGCCAGAGGAGCCAGCCTCTACAGACTGCATCATGCAAGGCCCAGGGCCAACTAAGGCCCCCAGTGGCCGGCCTAATGTGGCTGAGACCTCAGGAGGTAGTCGGCGCACCAATGGGGGCCACCTGACCCCCAGCTCTCAGATGTCATCCCGTCCACAGGAGGGATACCAGTCAGGGCCCGCCAAAGCAGTCAGGGGCCCACCACAACCTCCAGTGAGGCGGGACAGCCTTCAGGCCTCCAGAGCCCAACTCCTCAATGGAGAGCAGCGCAGGGCATCTGAGCCTGTGGTCCCCTTGCCACAGAAGGAGAAACTGAGCTTAGAGCCTGTGCTACCCGCAAGGAACCCTAATAGGTTCTGTTGCCTCAGTGGGCATGACCAAGTGACAAGTGAGGGCCATCAGAACTGTGAGTTCAGTCAGCCTCCTGAATCCAGCCAACAGGGCTCTGAGCATCTACTGATGCAGGCCTCAACCAAAGCTGTTGGATCCCCAAAAGCCTGTGACAGAGCTTCCAGCGTGGATTCCAACCCACTCAATGAGGCTTCTGCAGAGCTAGCTAAGGCTTCTTTTGGCAGACCTCCACATCTCATAGGACCCACAGGGCATCGCCATAGTGCCCCTGAACAGCTGCTGGCATCCCACCTGCAGCATGTGCACCTTGATACCAGGGGCAGCAAAGGGATGGAGCTCCCACCCGTACAGGATGGGCACCAGTGGACTCTGTCCCCTTTGCACAGCAGCCACAAAGGGAAGAAAAGTCCATGCCCCCCTACAGGAGGAACCCATGACCAGTCCAGCAAAGAAAGAAAGACCAGACAAGTGGATGACAGGTCTTTAGTTTTGGGACACCAGAGCCAAAGCAGTCCCCCACATGGAGAGGCTGATGGACACCCCTCAGAAAAAGGTTTCCTGGACCCAAACAGAACAAGCAGAGCAGCCAGTGAATTGGCCAACCAGCAACCCTCTGCCTCTGGCTCCCTTGTTCAACAAGCCACGGACTGTTCTTCAACCACTAAAGCAGCTAGTGGCACAGAGGCAGGTGAAGAAGGGGACAGCGAGCCCAAGGAGTGCAGCCGGATGGGTGGTAGGCGAAGTGGAGGGACCCGGGGCCGCTCGATCCAAAACCGGCGGAAGAGTGAGCGTTTTGCTACCAATCTGCGTAATGAAATTCAGAGGAGGAAGGCCCAGCTCCAGAAAAGCAAGGGTCCCTTGTCACAGCTGTGTGACACTAAGGAGCCAGTGGAAGAGACCCAGGAGCCCCCAGAAAGTCCTCCACTCACTGCCTCTAACACATCTCTTCTATCTTCATGTAAAAAACCTCCCAGCCCCAGAGACAAGCTCTTCAACAAAAGCATGATGCTCAGAGCTAGGTCTTCCGAGTGCCTCAGCCAAGCCCCTGAGAGCCATGAATCTAGGACAGGCTTAGAGGGACGAATAAGCCCTGGCCAGAGGCCTGGCCAGTCCTCTTTGGGCCTGAACACCTGGTGGAAAGCACCTGACCCATCCTCCTCAGACCCTGAGAAAGCACATGCTCACTGTGGAGTCCGTGGAGGTCATTGGAGATGGTCTCCAGAGCATAATTCACAGCCACTTGTGGCAGCAGCCATGGAAGGCCCTTCCAACCCAGGTGACAACAAGGAATTGAAGGCTTCTACTGCTCAAGCTGGGGAGGATGCCATCCTCTTGCCTTTTGCAGACAGAAGAAAGTTCTTTGAAGAGAGTAGCAAATCCTTATCTACATCTCATTTGCCAGGTTTAACCACTCATAGCAACAAGACTTTTACCCAGAGACCAAAACCTATAGACCAAAACTTCCAGCCAATGAGCTCCAGCTGTAGGGAATTGAGGCGCCATCCCATGGACCAATCATATCATTCCGCAGACCAACCATATCATGCCACAGACCAATCATATCATTCCATGTCACCCCTTCAGTCAGAAACTCCCACTTACTCAGAATGTTTTGCAAGCAAAGGTCTAGAAAATTCCATGTGTTGTAAGCCACTACACTGTGGTGATTTTGATTACCACAGGACCTGCTCTTACTCCTGCAGTGTTCAAGGAGCTCTAGTCCATGATCCTTGCATTTATTGTTCTGGGGAAATCTGCCCTGCCTTGCTAAAGAGAAATATGATGCCAAATTGCTACAACTGCCGGTGCCACCACCACCAATGCATTCGGTGTTCAGTTTGCTATCATAATCCTCAGCACAGTGCCCTCGAGGACAGCAGCTTGGCACCTGGCAACACTTGGAAACCCAGGAAGCTGACAGTGCAGGAATTTCCTGGGGACAAATGGAATCCAATAACAGGAAACAGGAAGACCAGCCAGTCAGGGAGGGAAATGGCTCATTCCAAGACTAGCTTTTCATGGGCAACCCCTTTCCATCCTTGCCTTGAGAACCCAGCACTGGACTTGTCAAGCTACCGAGCAATTTCTTCTCTTGACCTCCTTGGAGACTTCAAACATGCTTTGAAAAAATCAGAGGAAACTTCAGTTTATGAGGAGGGGAGCTCCCTTGCCTCCATGCCCCACCCACTGCGCAGCCGTGCCTTCTCAGAGAGTCACATCAGCTTGGCGCCCCAAAGCACCCGGGCCTGGGGGCAGCATAGGAGGGAGCTCTTTAGCAAAGGTGATGAGACCCAGTCGGATCTTCTCGGAGCCAGGAAGAAGGCCTTTCCTCCTCCTCGCCCTCCTCCTCCCAACTGGGAGAAGTACAGGCTCTTTCGTGCAGCCCAGCAGCAGAAGCAGCAACAGCAGCAGCAGAAGCAACAGGAGGAGGAGGAGGAGGAGGAAGAAGAAGAAGAAGAGGAAGAGGAAGAGGAGGAGGAGGAGGCAGAGGAGGAGGAAGAGGAGCTGCCACCCCAGTATTTCAGTTCAGAAACCTCTGGTTCCTGTGCTCTCAATCCTGAGGAGGTCCTAGAGCAGCCACAACCCCTCAGCTTTGGCCACCTGGAGGGCTCGAGACAGGGTTCACAAAGTGTCCCAGCAGAGCAAGAATCCTTTGCACTCCATTCCAGTGATTTCTTGCCTCCAATAAGGGGTCACTTGGGATCTCAACCTGAGCAGGCTCAGCCCCCTTGCTACTATGGCATTGGTGGGCTTTGGAGGACATCGGGACAGGAAGCCACTGAATCCGCCAAACAAGAGTTTCAGCACTTTTCGCCTCCTTCAGGGGCCCCAGGAATCCCTACCTCTTACTCAGCTTATTACAATATTTCTGTGGCCAAGGCAGAGCTGCTGAACAAACTGAAAGACCAACCTGAGATGGCAGAGATTGGCCTAGGAGAGGAGGAAGTTGACCATGAACTGGCTCAAAAAAAGATACAGCTTATCGAAAGCATCAGCAGAAAACTTTCTGTCTTGCGGGAGGCCCAGCGAGGGCTGCTAGAGGACATCAATGCCAATTCTGCCCTTGGGGAGGAGGTGGAGGCCAACTTAAAAGCCGTCTGCAAATCCAATGAATTTGAAAAGTACCACTTGTTTGTTGGGGACCTGGACAAAGTGGTCAACCTGTTGCTGTCACTCTCTGGACGACTGGCCCGGGTGGAGAATGCTCTGAACAGCATCGATTCAGAGGCCAACCAGGAGAAGTTGGTACTGATAGAGAAGAAGCAGCAGCTGACGGGGCAGTTGGCAGATGCCAAGGAGCTGAAGGAGCACGTGGACCGCCGGGAGAAGTTGGTGTTTGGCATGGTCTCCCGCTACCTGCCTCAGGACCAGCTCCAAGATTACCAGCACTTTGTCAAGATGAAATCTGCTCTCATCATTGAACAGCGAGAGCTGGAGGAGAAGATCAAGCTCGGGGAAGAGCAACTCAAATGTCTCAGGGAGAGTCTACTCCTGGGGCCCAGCAATTTCTAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFMENRPGSFQYVPVQLQGGAPWGFTLKGGLEHCEPLTVSKIEDGGKAALSQKMRTGDELVNINGTPLYGSRQEALILIKGSFRILKLIVRRRNAPVSRPHSWHVAKLLEGCPEAATTMHFPSEAFSLSWHSGCNTSDVCVQWCPLSWHCSTEKSSSIGSMESLEQPGQATYESHLLPIDQNMYPNQRDSAYSSFSASSNASDCALSLRPEEPASTDCIMQGPGPTKAPSGRPNVAETSGGSRRTNGGHLTPSSQMSSRPQEGYQSGPAKAVRGPPQPPVRRDSLQASRAQLLNGEQRRASEPVVPLPQKEKLSLEPVLPARNPNRFCCLSGHDQVTSEGHQNCEFSQPPESSQQGSEHLLMQASTKAVGSPKACDRASSVDSNPLNEASAELAKASFGRPPHLIGPTGHRHSAPEQLLASHLQHVHLDTRGSKGMELPPVQDGHQWTLSPLHSSHKGKKSPCPPTGGTHDQSSKERKTRQVDDRSLVLGHQSQSSPPHGEADGHPSEKGFLDPNRTSRAASELANQQPSASGSLVQQATDCSSTTKAASGTEAGEEGDSEPKECSRMGGRRSGGTRGRSIQNRRKSERFATNLRNEIQRRKAQLQKSKGPLSQLCDTKEPVEETQEPPESPPLTASNTSLLSSCKKPPSPRDKLFNKSMMLRARSSECLSQAPESHESRTGLEGRISPGQRPGQSSLGLNTWWKAPDPSSSDPEKAHAHCGVRGGHWRWSPEHNSQPLVAAAMEGPSNPGDNKELKASTAQAGEDAILLPFADRRKFFEESSKSLSTSHLPGLTTHSNKTFTQRPKPIDQNFQPMSSSCRELRRHPMDQSYHSADQPYHATDQSYHSMSPLQSETPTYSECFASKGLENSMCCKPLHCGDFDYHRTCSYSCSVQGALVHDPCIYCSGEICPALLKRNMMPNCYNCRCHHHQCIRCSVCYHNPQHSALEDSSLAPGNTWKPRKLTVQEFPGDKWNPITGNRKTSQSGREMAHSKTSFSWATPFHPCLENPALDLSSYRAISSLDLLGDFKHALKKSEETSVYEEGSSLASMPHPLRSRAFSESHISLAPQSTRAWGQHRRELFSKGDETQSDLLGARKKAFPPPRPPPPNWEKYRLFRAAQQQKQQQQQQKQQEEEEEEEEEEEEEEEEEEEEAEEEEEELPPQYFSSETSGSCALNPEEVLEQPQPLSFGHLEGSRQGSQSVPAEQESFALHSSDFLPPIRGHLGSQPEQAQPPCYYGIGGLWRTSGQEATESAKQEFQHFSPPSGAPGIPTSYSAYYNISVAKAELLNKLKDQPEMAEIGLGEEEVDHELAQKKIQLIESISRKLSVLREAQRGLLEDINANSALGEEVEANLKAVCKSNEFEKYHLFVGDLDKVVNLLLSLSGRLARVENALNSIDSEANQEKLVLIEKKQQLTGQLADAKELKEHVDRREKLVFGMVSRYLPQDQLQDYQHFVKMKSALIIEQRELEEKIKLGEEQLKCLRESLLLGPSNF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU59334
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Dear Simone, can you please make a mutant version (R146W) of the DU59055. Thank you!
• Low Temp: No