cDNA Clone Mindy2(KIAA1164)

• Plasmid: pGEX6P1 KIAA1164 241-504 I289A
• Parent Plasmid: pGEX6P1
• Expressed: GST KIAA1164 241-504 I289A
• Genbank: NM_001040450.1 GI:94721333
• Tag: GST

Sequence of Insert

GAATTCATGTCTGTGTATCACATCAAGTGGATCCAGTGGAAGGAAGAGAACACACCCATCATCACCCAGAATGAGAACGGACCCTGCCCCTTGCTGGCCATCCTCAATGTTTTGCTCCTGGCCTGGAAGGTGAAACTTCCACCGATGATGGAAGCCATAACTGCTGAGCAGCTGATGGAATATTTAGGAGATTACATGCTTGATGCAAAGCCAAAAGAAATTTCAGAAATTCAACGTTTAAATTATGAACAGAATATGAGTGATGCCATGGCAATTTTGCACAAACTACAGACAGGCCTGGATGTAAATGTAAGATTCACTGGTGTTCGAGTGTTTGAATATACACCAGAATGCATAGTATTTGATCTTCTTGATATTCCTTTGTACCATGGGTGGTTAGTAGACCCTCAGATTGATGACATTGTAAAAGCTGTTGGTAACTGCAGCTACAACCAACTAGTGGAGAAGATCATCTCTTGTAAACAGTCAGACAATAGTGAGCTGGTTAGTGAAGGCTTTGTAGCTGAGCAGTTTCTAAATAACACAGCCACTCAACTGACATACCATGGATTATGTGAACTAACTTCAACGGTTCAGGAAGGAGAACTTTGTGTGTTCTTTCGGAATAATCATTTTAGCACCATGACCAAATACAAGGGTCAACTGTATTTGTTGGTAACGGACCAGGGGTTTCTTACTGAAGAGAAAGTTGTTTGGGAAAGCCTACACAACGTAGATGGTGATGGAAATTTCTGTGACTCAGAATTTCATCTTCGACCTCCTTCAGATCCTGAAACTGTATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMSVYHIKWIQWKEENTPIITQNENGPCPLLAILNVLLLAWKVKLPPMMEAITAEQLMEYLGDYMLDAKPKEISEIQRLNYEQNMSDAMAILHKLQTGLDVNVRFTGVRVFEYTPECIVFDLLDIPLYHGWLVDPQIDDIVKAVGNCSYNQLVEKIISCKQSDNSELVSEGFVAEQFLNNTATQLTYHGLCELTSTVQEGELCVFFRNNHFSTMTKYKGQLYLLVTDQGFLTEEKVVWESLHNVDGDGNFCDSEFHLRPPSDPETV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU59998
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please mutate Ile289 to alanine in 55390
• Low Temp: No