cDNA Clone Fbxw7 (mouse)

• Synonyms: AGO; Cdc4; Fbw7; Fbwd6; Fbx30; Fbxw6; Fbxo30; SEL-10; 1110001A17Rik
• Plasmid: pCAGGS puro HA Fbxw7 mouse
• Parent Plasmid: pCAGGS puro HA
• Expressed: HA Fbxw7 mouse
• Genbank: NM_001177774.1
• Tag: HA
• Species: Mouse

Sequence of Insert

AAGCTTATGAATCAGGAACTGCTCTCTGTGGGCAGCAAAAGACGACGAACTGGAGGCTCTCTGAGAGGGAATGCTTCCTCAAGCCAGGTTGATGAGGGACAGATGAATCGCGTGGTTGAGGAGGATCCACAGCAGCAAGCGAGACATCAAGAGGAGGAGCACACTGCGCGGAATGGTGAACTTGTGGGTGCAGACCCTAGGCCTGGAGACCAGAACGATACCCAGCAAGGACAAGTGGAGGAAAATAATAACCGCTTTATTTCAGTAGATGAGGACTCTTCGGGAAATCAGGAAGAGCAAGAGGAAGATGAAGAGCATGCTGGGGAACAGGAGGAGGAAGAGGAGGAGGAGGAAGAGGAGGAGGAGATGGACCAGGAGAGTGATGATTTTGATCCGTCTGATGACAGTAGCAGAGAAGATGAACATACGCACAATAGCAATGTCACAAACTGCAGTAGTGTCTCGGACCTGCCCGCTCACCAGCTCTCCTCTCCATTCTATACAAAGACAACAAAAATGAAAAGAAAGTTGGACCATGGTTCTGAGGTTCGTTCCTTTTCTTTGGGAAAGAAACCATGCAAAGTCTCAGATTATACCAGTACCACTGGCCTTGTACCATGTTCAGCAACACCAACAACTTTTGGGGACCTGAGAGCAGCCAATGGGCAAGGGCAGCAGCGGCGGAGGATTACATCTGTCCAACCACCCACAGGCCTTCAAGAGTGGCTGAAAATGTTTCAGAGCTGGAGCGGACCAGAGAAGTTGCTGGCTTTAGATGAGCTCATTGACAGCTGTGAACCAACACAAGTGAAGCATATGATGCAAGTGATAGAGCCCCAGTTCCAGCGAGACTTCATCTCCTTGCTTCCTAAAGAGTTGGCACTCTATGTGCTTTCATTCCTGGAACCCAAAGACCTGCTGCAAGCGGCTCAGACTTGTCGATACTGGAGAATTTTGGCTGAGGATAACCTTCTCTGGAGAGAGAAATGTAAAGAAGAGGGGATTGATGAACCGTTGCACATCAAGAGAAGAAAAATAATAAAACCAGGTTTCATACACAGCCCATGGAAGAGTGCGTATATCAGACAGCACAGAATTGATACAAACTGGAGACGAGGAGAACTCAAATCTCCTAAGGTGCTGAAAGGGCATGATGACCATGTGATCACATGCCTACAGTTTTGTGGCAACCGCATAGTTAGTGGTTCTGATGACAACACTTTAAAAGTTTGGTCAGCGGTCACGGGCAAGTGTCTGAGAACGTTAGTGGGACATACAGGTGGAGTGTGGTCATCACAGATGAGAGACAATATCATCATCAGTGGATCGACTGACCGGACTCTCAAAGTGTGGAATGCTGAAACTGGAGAGTGTATACATACTTTATATGGGCACACTTCTACTGTACGGTGTATGCATCTCCATGAAAAAAGGGTTGTAAGCGGTTCTCGAGATGCCACTCTCAGGGTTTGGGATATTGAGACCGGCCAGTGTTTACACGTCCTGATGGGTCACGTAGCAGCGGTCCGCTGCGTTCAGTATGATGGCAGGAGGGTTGTTAGTGGAGCTTATGATTTTATGGTGAAGGTGTGGGATCCAGAGACTGAGACCTGTCTACACACGTTACAGGGACACACTAATAGAGTCTATTCATTACAGTTTGATGGCATCCATGTGGTGAGTGGATCTCTTGATACATCAATCCGAGTCTGGGATGTGGAGACAGGGAATTGTATTCACACGCTAACAGGACACCAGTCATTAACGAGTGGAATGGAACTCAAAGACAATATTCTTGTCTCTGGGAATGCAGATTCTACAGTTAAGATCTGGGATATCAAAACAGGACAGTGTTTACAAACTTTGCAAGGTCCCAGCAAGCATCAGAGCGCTGTGACCTGCTTACAGTTCAACAAGAACTTCGTAATTACCAGCTCAGACGACGGAACGGTCAAACTCTGGGACTTGAAAACGGGTGAATTTATCCGAAACCTCGTCACATTGGAGAGTGGGGGGAGCGGGGGAGTTGTGTGGCGGATCAGGGCCTCAAACACAAAGCTGGTGTGTGCAGTCGGGAGTCGGAATGGAACTGAGGAAACCAAGCTCCTGGTGCTGGACTTTGATGTGGACATGAAATGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSGKLMNQELLSVGSKRRRTGGSLRGNASSSQVDEGQMNRVVEEDPQQQARHQEEEHTARNGELVGADPRPGDQNDTQQGQVEENNNRFI
SVDEDSSGNQEEQEEDEEHAGEQEEEEEEEEEEEEMDQESDDFDPSDDSSREDEHTHNSNVTNCSSVSDLPAHQLSSPFYTKTTKMKRKLDHGSEVRSFS
LGKKPCKVSDYTSTTGLVPCSATPTTFGDLRAANGQGQQRRRITSVQPPTGLQEWLKMFQSWSGPEKLLALDELIDSCEPTQVKHMMQVIEPQFQRDFIS
LLPKELALYVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCKEEGIDEPLHIKRRKIIKPGFIHSPWKSAYIRQHRIDTNWRRGELKSPKVLKGHDDH
VITCLQFCGNRIVSGSDDNTLKVWSAVTGKCLRTLVGHTGGVWSSQMRDNIIISGSTDRTLKVWNAETGECIHTLYGHTSTVRCMHLHEKRVVSGSRDAT
LRVWDIETGQCLHVLMGHVAAVRCVQYDGRRVVSGAYDFMVKVWDPETETCLHTLQGHTNRVYSLQFDGIHVVSGSLDTSIRVWDVETGNCIHTLTGHQS
LTSGMELKDNILVSGNADSTVKIWDIKTGQCLQTLQGPSKHQSAVTCLQFNKNFVITSSDDGTVKLWDLKTGEFIRNLVTLESGGSGGVVWRIRASNTKL
VCAVGSRNGTEETKLLVLDFDVDMK*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCAGGS puro HA Fbxw7 mouse67.59 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61102
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Compared to reference there are two silent changes at E114 (a342g) and E180 a540g). There is also a SNP at N63D (a187g).
• Low Temp: No