cDNA Clone NUAK2

• Synonyms: NUAK family SNF1-like kinase 2
• Plasmid: pBabeD puro GFP-NUAK2
• Parent Plasmid: pBabeD puro GFP
• Expressed: GFP-NUAK2
• Genbank: NM_030952.3, NP_112214.3
• Tag: GFP
• Species: Human

Sequence of Insert

gtcgacATGCTGTGCTTTACTGCGCGCTCTGGTACTGCTGTGGCTCCCCG
TCCTGGTGCGGGACCTGTGCCCCGCGCTTCAGCCCTCCCCGCACAGCCTA
CTGATTCCCCTGCCGCCCTTGCTCACCTCCTGCTCGCCATGGAGTCGCTG
GTTTTCGCGCGGCGCTCCGGCCCCACTCCCTCGGCCGCAGAGCTAGCCCG
GCCGCTGGCGGAAGGGCTGATCAAGTCGCCCAAGCCCCTAATGAAGAAGC
AGGCGGTGAAGCGGCACCACCACAAGCACAACCTGCGGCACCGCTACGAG
TTCCTGGAGACCCTGGGCAAAGGCACCTACGGGAAGGTGAAGAAGGCGCG
GGAGAGCTCGGGGCGCCTGGTGGCCATCAAGTCAATCCGGAAGGACAAAA
TCAAAGATGAGCAAGATCTGATGCACATACGGAGGGAGATTGAGATCATG
TCATCACTCAACCACCCTCACATCATTGCCATCCATGAAGTGTTTGAGAA
CAGCAGCAAGATCGTGATCGTCATGGAGTATGCCAGCCGGGGCGACCTTT
ATGACTACATCAGCGAGCGGCAGCAGCTCAGTGAGCGCGAAGCTAGGCAT
TTCTTCCGGCAGATCGTCTCTGCCGTGCACTATTGCCATCAGAACAGAGT
TGTCCACCGAGATCTCAAGCTGGAGAACATCCTCTTGGATGCCAATGGGA
ATATCAAGATTGCTGACTTCGGcCTCTCCAACCTCTACCATCAAGGCAAG
TTCCTGCAGACATTCTGTGGGAGCCCCCTCTATGCCTCGCCAGAGATTGT
CAATGGGAAGCCCTACACAGGCCCAGAGGTGGACAGCTGGTCCCTGGGTG
TTCTCCTCTACATCCTGGTGCATGGCACCATGCCCTTTGATGGGCATGAC
CATAAGATCCTAGTGAAACAGATCAGCAACGGGGCCTACCGGGAGCCACC
TAAACCCTCTGATGCCTGTGGCCTGATCCGGTGGCTGTTGATGGTGAACC
CCACCCGCCGGGCCACCCTGGAGGATGTGGCCAGTCACTGGTGGGTCAAC
TGGGGCTACGCCACCCGAGTGGGAGAGCAGGAGGCTCCGCATGAGGGTGG
GCACCCTGGCAGTGACTCTGCCCGCGCCTCCATGGCTGACTGGCTCCGGC
GTTCCTCCCGCCCCCTCCTGGAGAATGGGGCCAAGGTGTGCAGCTTCTTC
AAGCAGCATGCACCTGGTGGGGGAAGCACCACCCCTGGCCTGGAGCGCCA
GCATTCGCTCAAGAAGTCCCGCAAGGAGAATGACATGGCCCAGTCTCTCC
ACAGTGACACGGCTGATGACACTGCCCATCGCCCTGGCAAGAGCAACCTC
AAGCTGCCAAAGGGCATTCTCAAGAAGAAGGTGTCAGCCTCTGCAGAAGG
GGTACAGGAGGACCCTCCGGAGCTCAGCCCAATCCCTGCGAGCCCAGGGC
AGGCTGCCCCCCTGCTCCCCAAGAAGGGCATTCTCAAGAAGCCCCGACAG
CGCGAGTCTGGCTACTACTCCTCTCCCGAGCCCAGTGAATCTGGGGAGCT
CTTGGACGCAGGCGACGTGTTTGTGAGTGGGGATCCCAAGGAGCAGAAGC
CTCCGCAAGCTTCAGGGCTcCTCCTCCATCGCAAAGGCATCCTCAAACTC
AATGGCAAGTTCTCCCAGACAGCCTTaGAGCTCGCGGCCCCCACCACCTT
CGGCTCCCTGGATGAACTCGCCCCACCTCGCCCCCTGGCCCGGGCCAGCC
GACCCTCAGGGGCTGTGAGCGAGGACAGCATCCTGTCCTCTGAGTCCTTT
GACCAGCTGGACTTGCCTGAACGGCTCCCAGAGCCCCCACTGCGGGGCTG
TGTGTCTGTGGACAACCTCACGGGGCTTGAGGAGCCCCCCTCAGAGGGCC
CTGGAAGCTGCCTGAGGCGCTGGCGGCAGGATCCTTTGGGGGACAGCTGC
TTTTCCCTGACAGACTGCCAGGAGGTGACAGCGACCTACCGACAGGCACT
GAGGGTCTGCTCAAAGCTCACCTGAgcggccgc

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSSEFEV
DMLCFTARSGTAVAPRPGAGPVPRASALPAQPTDSPAALAHLLLAMESLV
FARRSGPTPSAAELARPLAEGLIKSPKPLMKKQAVKRHHHKHNLRHRYEF
LETLGKGTYGKVKKARESSGRLVAIKSIRKDKIKDEQDLMHIRREIEIMS
SLNHPHIIAIHEVFENSSKIVIVMEYASRGDLYDYISERQQLSEREARHF
FRQIVSAVHYCHQNRVVHRDLKLENILLDANGNIKIADFGLSNLYHQGKF
LQTFCGSPLYASPEIVNGKPYTGPEVDSWSLGVLLYILVHGTMPFDGHDH
KILVKQISNGAYREPPKPSDACGLIRWLLMVNPTRRATLEDVASHWWVNW
GYATRVGEQEAPHEGGHPGSDSARASMADWLRRSSRPLLENGAKVCSFFK
QHAPGGGSTTPGLERQHSLKKSRKENDMAQSLHSDTADDTAHRPGKSNLK
LPKGILKKKVSASAEGVQEDPPELSPIPASPGQAAPLLPKKGILKKPRQR
ESGYYSSPEPSESGELLDAGDVFVSGDPKEQKPPQASGLLLHRKGILKLN
GKFSQTALELAAPTTFGSLDELAPPRPLARASRPSGAVSEDSILSSESFD
QLDLPERLPEPPLRGCVSVDNLTGLEEPPSEGPGSCLRRWRQDPLGDSCF
SLTDCQEVTATYRQALRVCSKLT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65105
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Full-length updated NCBI NM_030952.3 (ie. includes N-terminal extension); silent changes at G239 (GGT > GGC), L538 (CTG > CTC), L557 (TTG > TTA); ; Parental 33932
• Low Temp: No