cDNA Clone TRIB3

• Synonyms: tribbles homolog 3 isoform 1, tribbles pseudokinase 3, NIPK, SINK, C20orf97, SKIP3, TRB3
• Plasmid: pBabe.puro-DU C-Term GFP-TRIB3
• Parent Plasmid: pBabe.puro-DU C-Term GFP
• Expressed: TRIB3-GFP
• Genbank: NM_021158.4, NP_066981.2
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccATGCGAGCCACCCCTCTGGCTGCTCCTGCGGGTTCCCTGTCCAG
GAAGAAGCGGTTGGAGTTGGATGACAACTTAGATACCGAGCGTCCCGTCC
AGAAACGAGCTCGAAGTGGGCCCCAGCCCAGACTGCCCCCCTGCCTGTTG
CCCCTGAGCCCACCTACTGCTCCAGATCGTGCAACTGCTGTGGCCACTGC
CTCCCGTCTTGGGCCCTATGTCCTCCTGGAGCCCGAGGAGGGCGGGCGGG
CCTACCgGGCCCTGCACTGCCCTACAGGCACTGAGTATACCTGCAAGGTG
TACCCCGTCCAGGAAGCCCTGGCCGTGCTGGAGCCCTAcGCGCGGCTGCC
CCCGCACAAGCATGTGGCTCGGCCCACTGAGGTCCTGGCTGGTACCCAGC
TCCTCTACGCCTTTTTCACTCGGACCCATGGGGACATGCACAaCCTGGTG
CGAAGCCGCCACCGTATCCCTGAGCCTGAGGCTGCCGTGCTCTTCCGCCA
GATGGCCACCGCCCTGGCGCACTGTCACCAGCACGGTCTGGTCCTGCGTG
ATCTCAAGCTGTGTCGCTTTGTCTTCGCTGACCGTGAGAGGAAGAAGCTG
GTGCTGGAGAACCTGGAGGACTCCTGCGTGCTGACTGGGCCAGATGATTC
CCTGTGGGACAAGCACGCGTGCCCAGCCTACGTGGGACCTGAGATACTCA
GCTCACGGGCCTCATACTCGGGCAAGGCAGCCGATGTCTGGAGCCTGGGC
GTGGCGCTCTTCACCATGCTGGCCGGCCACTACCCCTTCCAGGACTCGGA
GCCTGTCCTGCTCTTCGGCAAGATCCGCCGCaGGGCCTACGCCTTGCCTG
CAGGCCTCTCGGCCCCTGCCCGCTGTCTGGTTCGCTGCCTCCTTCGTCGG
GAGCCAGCTGAACGGCTCACAGCCACAGGCATCCTCCTGCACCCCTGGCT
GCGACAGGACCCGATGCCCTTAGCtCCAACCCGATCCCATCTCTGGGAGG
CTGCCCAGGTGGTCCCTGATGGACTGGGGCTGGACGAAGCCAGGGAAGAG
GAGGGAGACAGAGAAGTGGTTCTGTATGGCgcggccgc

Amino Acid Sequence

MRATPLAAPAGSLSRKKRLELDDNLDTERPVQKRARSGPQPRLPPCLLPL
SPPTAPDRATAVATASRLGPYVLLEPEEGGRAYRALHCPTGTEYTCKVYP
VQEALAVLEPYARLPPHKHVARPTEVLAGTQLLYAFFTRTHGDMHNLVRS
RHRIPEPEAAVLFRQMATALAHCHQHGLVLRDLKLCRFVFADRERKKLVL
ENLEDSCVLTGPDDSLWDKHACPAYVGPEILSSRASYSGKAADVWSLGVA
LFTMLAGHYPFQDSEPVLLFGKIRRRAYALPAGLSAPARCLVRCLLRREP
AERLTATGILLHPWLRQDPMPLAPTRSHLWEAAQVVPDGLGLDEAREEEG
DREVVLYGAAAMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT
YGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAM
PEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILG
HKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPI
GDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65108
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Isoform 1. no stop codon; Contains listed SNPs Q84R and S146N, G276R; Silent changes at Y111 (TAT > TAC) and Ala323 (GCC > GCT); Parental 40432
• Low Temp: No