cDNA Clone RPG1/YBR079C

• Plasmid: p416 GDP 800-RPG1/YBR079C-300
• Parent Plasmid: p416-GDP

Sequence of Insert

ggatccATATTGATAACACTTTCAGAGATGCGTATAAATCTCCACTAAACATTCTTGTTATTGATTCGTTAGAGACTCTAGTTGATTGGGTACCAATTGGTCCAAGATTCTCTAATAACATTTTACAAATGCTAAAGGTTGCATTGAAGCGTAAACCCCCACAAGACCGTCGTTTATTGATCATGACTACTACATCAGCTTATTCGGTACTTCAACAAATGGATATCTTGAGTTGCTTCGACAATGAGATAGCAGTTCCAAATATGACCAATTTAGATGAATTGAACAACGTCATGATAGAATCAAACTTTCTTGACGATGCTGGTAGAGTTAAAGTTATTAATGAATTATCAAGGAGCTGTCCTAACTTCAATGTCGGTATTAAAAAGACCTTGACCAACATTGAAACCGCAAGGCACGATGAAGATCCCGTGAACGAGCTTGTTGAGTTGATGACCCAATCCGCATAATTATTTCAAATTTTTCATGTCCTTGTATTTTTATTCTTTATCCCTTCCAATCAGAAAGGATCTAGTGAACAAGTTCTTTCCTCTATGGTATATATTTTAGTGATAAATTTTATAAAATTATCAAAACCAAGGCATCCTTTCCTTTTATTCTGTCATTGGAATCTGCCTGTCATAATTATCACCCACCGGGTAAAGATGATAATTTTTCAGTCGCTTTGCCCGAGAAGCTTTGCCAGGTGAAAAATTTTTCTTGGTGAACTAAATCGAAAGTAGATATACTTACAACTATAGAGAGAGTTCCAAAATAAACCATACAAACGCCCAGGAAACATCAAGATGGCCCCCCCACCATTCCGTCCTGAAAATGCCATTAAGAGAGCCGATGAACTTATTTCTGTTGGCGAAAAACAAGCTGCTTTACAATCTTTGCATGATTTCATAACTGCCAGAAGAATCCGTTGGGCTACACCTTCAACTGTTGAGCCAGTCGTGTTTAAATTCTTGGAAATTGGTGTTGAACTAAAGAAAGGTAAGCTGCTAAAAGACGGTTTGCACCAGTACAAAAAATTAATTCAAGGATCTACCGAAGGTTTGGTTTCTGTCGGTGCCGTTGCACGTAAATTCATTGATTTAGTCGAATCTAAAATCGCTTCTGAACAAACTAGAGCTGACGAACTTCAAAAGCAAGAAATCGATGATGATTTGGAAGGTGGTGTTACCCCAGAAAATTTGCTGATTTCTGTTTACGAGTCAGATCAATCTGTGGCTGGTTTCAATGATGAAGCCATTACTTCATGGTTAAGATTTACTTGGGAATCATATCGTGCAGTCTTAGATTTATTGAGAAACAACGCTTTGTTGGAAATTACTTATTCTGGTGTCGTTAAAAAAACTATGCATTTCTGTTTGAAGTATCAGCGTAAAAACGAATTCAAGAGATTAGCTGAAATGTTACGTCAACATTTAGATGCTGCTAACTACCAACAAAGTAAATCAGGAAACAATTTAGTTGACTTGAGCGATGCTGATACCTTGCAACGTTATTTAGATCAACGTTTCCAACAAGTCGATGTTTCTGTCAAGTTGGAATTGTGGCACGAGGCTTATAGATCCATTGAGGATGTTTTCCACTTAATGAAAATATCCAAACGTGCTCCTAAACCATCTACTTTGGCTAATTACTACGAGAATCTCGTAAAGGTCTTCTTTGTTTCCGGTGATCCATTGTTGCACACTACTGCCTGGAAGAAGTTTTATAAGTTATATTCTACCAATCCAAGAGCCACGGAAGAAGAATTCAAAACATATTCTTCTACAATTTTCCTATCCGCTATTTCTACCCAACTAGACGAAATTCCATCTATTGGCTACGATCCTCATTTACGTATGTACCGTTTATTAAACTTGGACGCCAAGCCAACTAGAAAGGAAATGTTGCAATCCATTATTGAAGACGAATCCATTTATGGTAAAGTTGACGAGGAATTGAAAGAATTGTACGATATCATTGAAGTTAACTTTGATGTCGACACAGTGAAACAACAGCTGGAAAATTTATTAGTCAAATTGTCTTCTAAAACCTACTTCAGCCAATACATCGCACCTTTAAGAGACGTCATTATGAGAAGGGTTTTTGTTGCTGCCTCTCAAAAATTCACTACTGTCTCCCAGTCCGAATTATACAAGCTCGCTACCCTACCTGCTCCGTTAGACTTATCTGCTTGGGACATCGAAAAATCGCTGTTACAAGCTGCTGTGGAAGATTATGTTTCAATTACCATCGATCATGAATCTGCTAAGGTTACATTCGCTAAAGATCCATTTGACATTTTCGCATCAACTGCTTCTAAAGAAGTCTCTGAAGAAGAAAACACAGAGCCAGAAGTCCAAGAAGAAAAGGAGGAAACTGACGAAGCTCTTGGGCCACAAGAAACTGAAGACGGTGAAGAAAAGGAAGAAGAATCTGATCCTGTTATTATCCGCAATTCTTACATCCACAACAAGTTGCTTGAATTATCCAATGTCCTACACGATGTTGACAGTTTCAACAATGCTTCATACATGGAAAAGGTTAGAATTGCCCGTGAAACTTTAATTAAGAAGAACAAGGATGACCTTGAAAAGATTTCCAAGATTGTTGACGAACGTGTCAAGAGATCTCAAGAGCAAAAGCAAAAACATATGGAACATGCTGCCCTACATGCTGAACAGGATGCTGAGGTCAGACAACAGCGTATTTTGGAAGAAAAAGCTGCTATTGAAGCTAAATTGGAAGAAGAGGCTCACCGTCGCTTAATTGAAAAGAAGAAACGTGAATTTGAAGCTATCAAAGAAAGAGAAATCACAAAGATGATTACTGAGGTGAATGCTAAGGGACATGTTTACATTGACCCTAACGAAGCTAAAAGCTTAGATCTTGACACCATTAAACAGGTTATTATAGCTGAGGTATCAAAGAACAAGAGTGAATTGGAAAGTCGTATGGAATATGCTATGAAGAAGTTAGATCATACGGAAAGAGCTTTGAGAAAGGTCGAATTGCCATTGCTGCAAAAAGAAGTTGACAAGCTACAAGAAACAGACACAGCAAATTATGAAGCTATGAAGAAAAAAATTGTCGACGCCGCCAAGGCCGAATACGAAGCCAGAATGGCTGATCGTAAGAACTTGGTCATGGTCTATGATGATTATTTGAAGTTCAAGGAGCATGTTTCGGGCACTAAGGAAAGCGAATTAGCTGCCATCCGCAACCAAAAGAAGGCTGAATTGGAAGCTGCCAAAAAGGCCAGAATTGAAGAAGTTCGTAAGCGTCGTTACGAGGAAGCCATTGCAAGGCGTAAGGAAGAAATTGCAAATGCTGAAAGACAGAAGCGTGCTCAAGAACTCGCTGAGGCTACCCGTAAACAAAGAGAAATCGAAGAAGCTGCTGCCAAGAAATCAACTCCATACTCTTTCCGCGCAGGAAACAGGGAACCTCCAAGTACACCAAGCACTCTACCAAAGGCGACCGTTTCTCCAGATAAAGCTAAATTGGATATGATCGCTCAAAAACAAAGAGAAATGGAAGAAGCCATCGAACAGAGACTAGCCGGTAGAACAGCTGGTGGGTCCTCCCCAGCTACTCCAGCTACTCCAGCAACCCCAGCAACCCCAACTCCATCTTCTGGTCCAAAGAAAATGACTATGGCTGAAAAGTTGAGAGCCAAGAGATTGGCCAAGGGGGGCAGGTAACAGACAACGCAAAATGATCTACTTAATTTATACCGTTTTTTATACGTATAAGAGTATGTGTACATAATAACAATTTATTAGACTATTTCTTTCTGATTGCTTTTTCATGTCAAAGGAAGGAGCAGAAGAGCAGTAAAGATGGCAGTTATTTTAAAAACGAATTTCGAGAAATAGTTGGTATAAATAACTATAAATAACGTTTTACTGTTTTAAAAAAGCGATCCGTTAAAAGGCCCTAACGATTCTTTAGAAACTTGAAACGTTAGCGGAAGAGCACTTTGTTTTAAACCGTAGTAAGAAcccggg

Amino Acid Sequence

MAPPPFRPENAIKRADELISVGEKQAALQSLHDFITARRIRWATPSTVEPVVFKFLEIGVELKKGKLLKDGLHQYKKLIQGSTEGLVSVGAVARKFIDLVESKIASEQTRADELQKQEIDDDLEGGVTPENLLISVYESDQSVAGFNDEAITSWLRFTWESYRAVLDLLRNNALLEITYSGVVKKTMHFCLKYQRKNEFKRLAEMLRQHLDAANYQQSKSGNNLVDLSDADTLQRYLDQRFQQVDVSVKLELWHEAYRSIEDVFHLMKISKRAPKPSTLANYYENLVKVFFVSGDPLLHTTAWKKFYKLYSTNPRATEEEFKTYSSTIFLSAISTQLDEIPSIGYDPHLRMYRLLNLDAKPTRKEMLQSIIEDESIYGKVDEELKELYDIIEVNFDVDTVKQQLENLLVKLSSKTYFSQYIAPLRDVIMRRVFVAASQKFTTVSQSELYKLATLPAPLDLSAWDIEKSLLQAAVEDYVSITIDHESAKVTFAKDPFDIFASTASKEVSEEENTEPEVQEEKEETDEALGPQETEDGEEKEEESDPVIIRNSYIHNKLLELSNVLHDVDSFNNASYMEKVRIARETLIKKNKDDLEKISKIVDERVKRSQEQKQKHMEHAALHAEQDAEVRQQRILEEKAAIEAKLEEEAHRRLIEKKKREFEAIKEREITKMITEVNAKGHVYIDPNEAKSLDLDTIKQVIIAEVSKNKSELESRMEYAMKKLDHTERALRKVELPLLQKEVDKLQETDTANYEAMKKKIVDAAKAEYEARMADRKNLVMVYDDYLKFKEHVSGTKESELAAIRNQKKAELEAAKKARIEEVRKRRYEEAIARRKEEIANAERQKRAQELAEATRKQREIEEAAAKKSTPYSFRAGNREPPSTPSTLPKATVSPDKAKLDMIAQKQREMEEAIEQRLAGRTAGGSSPATPATPATPATPTPSSGPKKMTMAEKLRAKRLAKGGR*

• Vector Type: Yeast
• Antibiotic: Amp; Kan
• DU Number: DU66064
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: RPG1/YBR079C (ID: 852371) (including 800bp of the 5'UTR and 300 bp of the 3'UTR) in p416-GPD (DU66025) vector.
• Low Temp: No