cDNA Clone PRE9/YGR135W

• Plasmid: p416 GPD 800-PRE9/YGR135W -300
• Parent Plasmid: p416 GPD
• Species: Yeast

Sequence of Insert

ggatccCGAAGAAGAGAATGAAGACGGCGAGAAAATCTCCTTGTACTCCCTTGGTTTGGTTAGGTTGATCTGTTACATGGTGCAGACACTCATCGCGAACGACAAGTTTTTTTTTACCAAATCAGAATGCACTTTTGAGCTTCAAACTTTACTGATGACATGGATTGGCATCCTTCCTGAAGCCAAAGATTTATTCTTTAAGATCAAGACAAGGCTCGCCATGGAAGAAGAGGATAGTGCCGATACTATGCAGCATGAGGGCAGGAAGAACTCTGACATAGAGAAGAAGCTAAACGCTAAGCCAGCTTCTGAACTGAATCTGAAATTGTTAAACCTGTTTCCCTCAAAGCCTGCAAACAAAGACGATAGTTCCCCTATTAACACGTTGCGTAGTTTTATCGCTGATTACTCCTTCGACACCCAGGTGAACCCTCCAGGAAGAAGGGTGGTGTTCTACGATGGTAAGATTTTGCCATTGCCCAAAGCCGATAAGCCTATCCCACTTCATGAATATATAACACTCGCAGAGCTCGATGTTGGAGACAGTGAGTGAGCAGTGAATTGCTCATGTTTTCTCTGCATCCTCATTTAATGACAATTAGCCATGTAATAACATCTTGAGGCAGTTAAATATTCGTTACCCTGCAGGTGGCAAAAAATTTATAGAATAAAAGCATAAAAAGATGGATATCTATGTAATAAGGAAACATTGGCAGAGCGAAGAGAACAGACTGCTTTCTATAAAAAGTTTTCGATCAGTCTCTATTTTAATAATTGATTATTGGATATAGTTAGTAGTGTTAAACATGGGTTCCAGAAGATACGATTCCAGGACAACAATTTTCTCCCCTGAGGGACGTCTATATCAGGTTGAATACGCGCTAGAATCCATTTCACATGCAGGTACCGCAATTGGGATTATGGCATCTGATGGGATTGTTCTTGCAGCAGAACGCAAAGTCACAAGTACTTTACTAGAACAAGACACCTCTACCGAAAAACTTTATAAGTTAAACGATAAAATTGCGGTTGCCGTTGCTGGACTGACTGCAGATGCAGAAATTCTAATAAATACGGCTAGAATTCACGCTCAAAATTACCTTAAAACCTATAATGAAGATATACCAGTAGAAATTTTGGTGAGAAGGCTAAGTGATATAAAACAAGGTTACACGCAACATGGTGGTTTAAGACCATTTGGTGTGTCCTTTATCTACGCCGGTTATGACGATAGATACGGTTACCAATTGTATACATCTAATCCATCGGGAAACTATACAGGGTGGAAGGCTATTAGTGTTGGCGCTAACACATCAGCAGCACAAACCCTACTTCAAATGGACTACAAGGATGATATGAAAGTCGATGATGCCATTGAACTGGCTTTAAAAACGTTATCCAAAACTACCGACAGTAGCGCGCTGACTTATGACAGGTTGGAATTTGCTACTATCAGAAAGGGTGCTAATGACGGAGAAGTGTATCAGAAGATTTTCAAGCCTCAAGAGATAAAGGATATATTGGTAAAGACTGGTATTACCAAGAAGGATGAAGACGAAGAAGCTGATGAAGATATGAAATAAGGTTGGAAAGTATTGTTTGACTTCATGCTTATATAAATATGTACGCATAGAAACATATCTCTAAAATTAAAAGTGAAAGAAAAAAATCGCTAAGATTCCCCTTTCGGGGGAAGGCTGAAGAAACTTTTTTTTGCGCAAATTTCAAGATCGGAATCGCTCGAGAGGCAAATATAAAAAAGGGCCTGCTCGTTTGGCCTACTTGTTGTTGGATTCTAACTCCAATCTAATTTTGGCAGCACTTCAAAAATAAACAAAAGCGATGTCTGCGTCACTAGTGAATCGATCATTGAAAAATATAAGcccggg

Amino Acid Sequence

MGSRRYDSRTTIFSPEGRLYQVEYALESISHAGTAIGIMASDGIVLAAERKVTSTLLEQDTSTEKLYKLNDKIAVAVAGLTADAEILINTARIHAQNYLKTYNEDIPVEILVRRLSDIKQGYTQHGGLRPFGVSFIYAGYDDRYGYQLYTSNPSGNYTGWKAISVGANTSAAQTLLQMDYKDDMKVDDAIELALKTLSKTTDSSALTYDRLEFATIRKGANDGEVYQKIFKPQEIKDILVKTGITKKDEDEEADEDMK*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU66084
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: PRE9/YGR135W (ID: 853036) (including 800bp of the 5'UTR and 300 bp of the 3'UTR) in p416-GPD (DU66025) vector.
• Low Temp: No