cDNA Clone TRIB3

• Synonyms: tribbles homolog 3 isoform 1, tribbles pseudokinase 3, NIPK, SINK, C20orf97, SKIP3, TRB3
• Plasmid: pBabe.puro-GFP-TRIB3
• Parent Plasmid: pBabe.puro-GFP
• Expressed: GFP-TRIB3
• Genbank: NM_021158.4, NP_066981.2
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccATGCGAGCCACCCCTCTGGCTGCTCCTGCGGGTTCCCTGTCCAG
GAAGAAGCGGTTGGAGTTGGATGACAACTTAGATACCGAGCGTCCCGTCC
AGAAACGAGCTCGAAGTGGGCCCCAGCCCAGACTGCCCCCCTGCCTGTTG
CCCCTGAGCCCACCTACTGCTCCAGATCGTGCAACTGCTGTGGCCACTGC
CTCCCGTCTTGGGCCCTATGTCCTCCTGGAGCCCGAGGAGGGCGGGCGGG
CCTACCgGGCCCTGCACTGCCCTACAGGCACTGAGTATACCTGCAAGGTG
TACCCCGTCCAGGAAGCCCTGGCCGTGCTGGAGCCCTAcGCGCGGCTGCC
CCCGCACAAGCATGTGGCTCGGCCCACTGAGGTCCTGGCTGGTACCCAGC
TCCTCTACGCCTTTTTCACTCGGACCCATGGGGACATGCACAaCCTGGTG
CGAAGCCGCCACCGTATCCCTGAGCCTGAGGCTGCCGTGCTCTTCCGCCA
GATGGCCACCGCCCTGGCGCACTGTCACCAGCACGGTCTGGTCCTGCGTG
ATCTCAAGCTGTGTCGCTTTGTCTTCGCTGACCGTGAGAGGAAGAAGCTG
GTGCTGGAGAACCTGGAGGACTCCTGCGTGCTGACTGGGCCAGATGATTC
CCTGTGGGACAAGCACGCGTGCCCAGCCTACGTGGGACCTGAGATACTCA
GCTCACGGGCCTCATACTCGGGCAAGGCAGCCGATGTCTGGAGCCTGGGC
GTGGCGCTCTTCACCATGCTGGCCGGCCACTACCCCTTCCAGGACTCGGA
GCCTGTCCTGCTCTTCGGCAAGATCCGCCGCaGGGCCTACGCCTTGCCTG
CAGGCCTCTCGGCCCCTGCCCGCTGTCTGGTTCGCTGCCTCCTTCGTCGG
GAGCCAGCTGAACGGCTCACAGCCACAGGCATCCTCCTGCACCCCTGGCT
GCGACAGGACCCGATGCCCTTAGCtCCAACCCGATCCCATCTCTGGGAGG
CTGCCCAGGTGGTCCCTGATGGACTGGGGCTGGACGAAGCCAGGGAAGAG
GAGGGAGACAGAGAAGTGGTTCTGTATGGCTAGGCGGCCGC

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMRATP
LAAPAGSLSRKKRLELDDNLDTERPVQKRARSGPQPRLPPCLLPLSPPTA
PDRATAVATASRLGPYVLLEPEEGGRAYRALHCPTGTEYTCKVYPVQEAL
AVLEPYARLPPHKHVARPTEVLAGTQLLYAFFTRTHGDMHNLVRSRHRIP
EPEAAVLFRQMATALAHCHQHGLVLRDLKLCRFVFADRERKKLVLENLED
SCVLTGPDDSLWDKHACPAYVGPEILSSRASYSGKAADVWSLGVALFTML
AGHYPFQDSEPVLLFGKIRRRAYALPAGLSAPARCLVRCLLRREPAERLT
ATGILLHPWLRQDPMPLAPTRSHLWEAAQVVPDGLGLDEAREEEGDREVV
LYG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65151
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Isoform 1. ; Contains listed SNPs Q84R and S146N, G276R; Silent changes at Y111 (TAT > TAC) and Ala323 (GCC > GCT); Parental 33932
• Low Temp: No