cDNA Clone LRRK1

• Synonyms: RIPK6; Roco1
• Plasmid: pcDNA5D FRT/TO GFP LRRK1 S405E
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP-LRRK1 S405E
• Genbank: NM_024652
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccATGGCTGGCATGTCGCAAAGACCCCCCAGCATGTACTGGTGTGTGGGGCCGGAGGAGTCAGCTGTGTGTCCAGAACGTGCCATGGAGACGCTTAACGGTGCCGGGGACACGGGCGGCAAGCCGTCCACGCGGGGCGGTGACCCTGCAGCGCGGTCCCGCAGGACGGAAGGCATCCGCGCCGCGTACAGGCGGGGAGACCGCGGCGGCGCCCGGGACCTGCTGGAGGAGGCCTGCGACCAGTGCGCGTCCCAGCTGGAAAAGGGCCAGCTTCTGAGCATCCCGGCAGCCTATGGGGATCTGGAGATGGTCCGCTACCTACTCAGCAAGAGACTGGTGGAGCTGCCCACCGAGCCCACGGATGACAACCCAGCCGTGGTGGCAGCGTATTTTGGACACACGGCAGTTGTGCAGGAATTGCTTGAGTCCTTACCAGGTCCCTGCAGTCCCCAGCGGCTTCTGAACTGGATGCTGGCCTTGGCTTGCCAGCGAGGGCACCTGGGGGTTGTGAAGCTCCTGGTCCTGACGCACGGGGCTGACCCGGAGAGCTACGCTGTCAGGAAGAATGAGTTCCCTGTCATCGTGCGCTTGCCCCTGTATGCGGCCATCAAGTCAGGGAATGAAGACATTGCAATATTCCTGCTTCGGCATGGGGCCTATTTCTGTTCCTACATCTTGCTGGATAGTCCTGACCCCAGCAAACATCTGCTGAGAAAGTACTTCATTGAAGCCAGTCCCTTGCCCAGCAGTTATCCGGGAAAAACAGCTCTCCGTGTGAAATGGTCCCATCTCAGACTGCCCTGGGTAGACCTAGACTGGCTCATAGACATCTCCTGCCAGATCACGGAGCTCGACCTTTCTGCCAACTGCCTGGCGACCCTCCCCTCGGTTATCCCCTGGGGCCTCATCAATCTCCGGAAGCTGAACCTCTCCGACAACCACCTGGGGGAGCTGCCTGGCGTGCAGTCATCGGACGAAATCATCTGTTCCAGGCTACTTGAAATTGACATTTCCAGCAACAAGTTGTCCCACCTCCCTCCTGGATTCTTGCACCTCTCAAAACTTCAAAAACTGACAGCTTCAAAAAATTGTTTAGAAAAATTGTTCGAAGAAGAAAATGCCACTAACTGGATAGGTTTACGGAAGCTACAGGAACTTGATATATCTGACAATAAATTGACAGAACTCCCTGCCCTGTTCCTTCACTCTTTCAAGgagCTCAATTCTCTGAATGTCTCCAGAAACAACCTGAAGGTGTTTCCAGATCCCTGGGCCTGCCCTTTGAAATGTTGTAAAGCTTCCAGAAATGCCCTGGAATGTCTGCCAGACAAAATGGCTGTCTTTTGGAAAAATCACCTGAAGGATGTGGATTTCTCAGAAAACGCACTCAAAGAAGTTCCCCTGGGACTTTTCCAGCTTGATGCCCTCATGTTCTTGAGGTTACAGGGGAACCAGCTGGCGGCACTTCCACCTCAAGAGAAGTGGACCTGCAGGCAGCTCAAAACCCTGGATCTCTCCAGAAACCAACTTGGCAAAAATGAAGATGGACTGAAAACGAAGCGTATTGCCTTTTTCACCACCAGAGGTCGCCAGCGCTCCGGGACTGAGGCAGCAAGTGTGCTGGAATTTCCGGCCTTCCTAAGTGAGTCTTTGGAAGTCCTTTGCCTGAACGACAACCACCTCGACACAGTCCCTCCCTCGGTTTGCCTACTGAAGAGCTTATCAGAGCTCTACTTGGGAAACAACCCTGGCCTCCGGGAGCTCCCTCCTGAGCTGGGGCAGCTGGGCAACCTCTGGCAGCTGGACACTGAAGACCTGACCATCAGCAATGTGCCTGCAGAAATCCAAAAAGAAGGCCCCAAAGCAATGCTGTCTTACCTGCGTGCTCAGCTGCGGAAAGCGGAAAAGTGCAAGCTGATGAAGATGATCATCGTGGGTCCCCCGCGCCAGGGCAAGTCCACCCTCCTGGAGATCTTACAGACGGGGAGGGCCCCCCAGGTGGTGCATGGAGAGGCCACCATCAGGACCACCAAGTGGGAGCTCCAGAGGCCGGCTGGCTCAAGAGCCAAGGTTGAGTCCGTGGAGTTCAACGTCTGGGACATCGGGGGACCGGCCAGCATGGCCACTGTCAACCAGTGCTTCTTCACGGACAAGGCCCTGTACGTGGTGGTCTGGAACCTGGCGCTGGGGGAGGAGGCCGTGGCCAACCTCCAGTTCTGGCTGCTCAACATCGAGGCCAAGGCCCCAAACGCCGTGGTGCTGGTGGTCGGGACGCACCTGGATTTAATTGAAGCCAAGTTCCGTGTGGAAAGGATTGCAACGCTGCGTGCCTATGTGCTGGCACTCTGCCGCTCCCCCTCCGGCTCCAGGGCCACAGGCTTCCCAGACATCACCTTCAAACACTTACATGAGATTTCCTGCAAGAGCCTGGAAGGTCAGGAAGGGCTGCGACAGCTGATTTTCCACGTCACGTGCAGCATGAAGGACGTGGGCAGCACCATCGGCTGCCAGCGACTGGCAGGGCGGCTGATCCCCAGGAGCTACCTGAGCCTGCAGGAGGCCGTGCTGGCAGAGCAGCAGCGCCGCAGCCGGGACGACGACGTGCAGTACCTGACGGACAGGCAGCTGGAGCAGCTGGTGGAGCAGACGCCCGACAACGACATCAAGGACTACGAGGACCTGCAGTCAGCCATCAGCTTCCTCATAGAAACCGGCACCCTGCTCCATTTCCCGGACACCAGCCACGGCCTGAGGAACCTCTACTTCCTCGACCCTATTTGGCTCTCCGAATGTCTGCAGAGGATCTTTAATATTAAGGGCTCTCGGTCAGTGGCCAAGAATGGGGTGATCAGAGCAGAAGACCTCAGGATGCTGCTGGTGGGGACTGGCTTCACGCAGCAGACGGAAGAGCAGTACTTCCAGTTCCTGGCCAAGTTTGAGATCGCCCTGCCCGTCGCCAATGACAGCTACCTCCTGCCCCATCTCCTTCCATCTAAACCTGGCCTGGACACCCACGGTATGCGGCACCCCACAGCCAACACCATTCAGAGGGTATTTAAGATGAGCTTCGTTCCCGTTGGCTTCTGGCAAAGGTTTATAGCACGGATGCTGATCAGCCTGGCGGAGATGGACCTGCAGCTTTTTGAAAACAAGAAGAATACTAAAAGCAGGAACAGGAAAGTCACCATTTACAGTTTTACAGGAAACCAGAGAAATCGCTGTAGCACATTCAGAGTGAAAAGAAATCAGACCATCTATTGGCAGGAAGGGCTCCTGGTCACTTTTGATGGGGGCTACCTCAGTGTGGAATCTTCCGACGTGAACTGGAAAAAGAAGAAAAGCGGAGGAATGAAAATTGTTTGCCAATCAGAAGTGAGGGACTTCTCAGCCATGGCTTTCATCACGGACCACGTCAATTCCTTGATTGATCAGTGGTTTCCCGCCCTGACAGCCACAGAGAGCGACGGGACGCCACTCATGGAGCAGTACGTGCCCTGCCCGGTCTGCGAGACAGCCTGGGCCCAGCACACGGACCCCAGTGAGAAATCAGAGGATGTGCAGTACTTCGACATGGAAGACTGTGTCCTGACGGCCATCGAGCGGGACTTCATCTCCTGCCCCAGACACCCGGACCTCCCCGTGCCGCTGCAGGAGCTGGTCCCTGAACTGTTCATGACCGACTTCCCGGCCAGGCTCTTCCTGGAGAACAGCAAGCTGGAGCACAGCGAGGACGAGGGCAGCGTCCTGGGCCAGGGCGGCAGTGGCACCGTCATCTACCGGGCCCGGTACCAGGGCCAGCCTGTGGCCGTCAAGCGCTTCCACATCAAAAAATTCAAGAACTTTGCTAACGTACCGGCAGACACCATGCTGAGGCACCTGCGGGCCACCGATGCCATGAAGAACTTCTCCGAGTTCCGGCAGGAGGCCAGCATGCTGCACGCGCTGCAGCACCCCTGCATCGTGGCGCTCATCGGCATCAGCATCCACCCGCTCTGCTTCGCCCTGGAGCTCGCGCCGCTCAGCAGCCTCAACACCGTGCTGTCCGAGAACGCCAGAGATTCTTCCTTTATACCCCTGGGACACATGCTCACCCAAAAAATAGCCTACCAGATCGCCTCGGGCCTGGCCTACCTGCACAAGAAAAACATCATCTTCTGTGACCTGAAGTCGGACAACATTCTGGTGTGGTCCCTTGACGTCAAGGAGCACATCAACATCAAGCTATCTGACTACGGGATTTCGAGGCAGTCATTCCATGAGGGCGCCCTAGGCGTGGAGGGCACTCCTGGCTACCAGGCCCCAGAGATCAGGCCTCGCATTGTATATGATGAGAAGGTAGATATGTTCTCCTATGGAATGGTGCTCTACGAGTTGCTGTCAGGACAGCGCCCTGCACTGGGCCACCACCAGCTCCAGATTGCCAAGAAGCTGTCCAAGGGCATCCGCCCGGTTCTGGGGCAGCCGGAGGAAGTGCAGTTCCGGCGACTGCAGGCGCTCATGATGGAGTGCTGGGACACTAAGCCAGAGAAGCGACCGCTGGCCCTGTCGGTGGTGAGCCAGATGAAGGACCCGACTTTTGCCACCTTCATGTATGAACTGTGCTGTGGGAAGCAGACAGCCTTCTTCTCATCCCAGGGCCAGGAGTACACCGTGGTGTTTTGGGATGGAAAAGAGGAGTCCAGGAACTACACGGTGGTGAACACAGAGAAGGGCCTCATGGAGGTGCAGAGGATGTGCTGCCCTGGGATGAAGGTGAGCTGCCAGCTCCAGGTCCAGAGATCCCTGTGGACAGCCACCGAGGACCAGAAAATCTACATCTACACCCTCAAGGGCATGTGCCCCTTAAACACACCCCAACAGGCCTTGGATACTCCAGCTGTCGTCACCTGCTTCTTGGCCGTGCCTGTTATTAAAAAGAATTCCTACCTGGTCTTAGCGGGCCTCGCCGATGGGCTTGTGGCTGTGTTTCCCGTGGTGCGGGGCACCCCAAAGGACAGCTGCTCCTACCTGTGCTCACACACAGCCAACAGGTCCAAGTTCAGCATCGCGGATGAAGACGCACGGCAGAACCCCTACCCAGTGAAGGCCATGGAGGTGGTCAACAGCGGCTCTGAGGTCTGGTACAGCAATGGGCCGGGCCTCCTTGTCATCGACTGTGCCTCCCTGGAGATCTGCAGGCGGCTGGAGCCCTACATGGCCCCCTCCATGGTTACGTCAGTCGTGTGCAGCTCTGAGGGCAGAGGGGAGGAGGTCGTCTGGTGCCTGGATGACAAGGCCAACTCCTTGGTGATGTACCACTCCACCACCTACCAGCTGTGTGCCCGGTACTTCTGCGGGGTCCCCAGCCCCCTCAGGGACATGTTTCCCGTGCGGCCCTTGGACACGGAACCCCCGGCAGCCAGCCACACGGCCAACCCAAAGGTGCCTGAGGGGGACTCCATCGCGGACGTGAGCATCATGTACAGTGAGGAGCTGGGCACGCAGATCCTGATCCACCAGGAATCACTCACTGACTACTGCTCCATGTCCTCCTACTCCTCATCCCCACCCCGCCAGGCTGCCAGGTCCCCCTCAAGCCTCCCCAGCTCCCCAGCAAGTTCTTCCAGTGTGCCTTTCTCCACCGACTGCGAGGACTCAGACATGCTACATACGCCCGGTGCTGCCTCCGACAGGTCTGAGCATGACCTGACCCCCATGGACGGGGAGACCTTCAGCCAGCACCTGCAGGCCGTGAAGATCCTCGCCGTCAGAGACCTCATTTGGGTCCCCAGGCGCGGTGGAGATGTTATCGTCATTGGCCTGGAGAAGGATTCTGGCGCCCAGCGGGGCCGAGTCATTGCCGTCTTAAAAGCCCGAGAGCTGACTCCGCATGGGGTGCTGGTGGATGCTGCCGTGGTGGCAAAGGACACTGTTGTGTGCACCTTTGAAAATGAAAACACAGAGTGGTGCCTGGCCGTCTGGAGGGGCTGGGGCGCCAGGGAGTTCGACATTTTCTACCAGTCCTACGAGGAGCTGGGCCGGCTGGAGGCTTGCACTCGCAAGAGAAGGTAAgcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMAGMSQRPPSMYWCVGPEESAVCPERAMETLNGAGDTGGKPSTRGGDPAARSRRTEGIRAAYRRGDRGGARDLLEEACDQCASQLEKGQLLSIPAAYGDLEMVRYLLSKRLVELPTEPTDDNPAVVAAYFGHTAVVQELLESLPGPCSPQRLLNWMLALACQRGHLGVVKLLVLTHGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLLRHGAYFCSYILLDSPDPSKHLLRKYFIEASPLPSSYPGKTALRVKWSHLRLPWVDLDWLIDISCQITELDLSANCLATLPSVIPWGLINLRKLNLSDNHLGELPGVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNCLEKLFEEENATNWIGLRKLQELDISDNKLTELPALFLHSFKELNSLNVSRNNLKVFPDPWACPLKCCKASRNALECLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLRLQGNQLAALPPQEKWTCRQLKTLDLSRNQLGKNEDGLKTKRIAFFTTRGRQRSGTEAASVLEFPAFLSESLEVLCLNDNHLDTVPPSVCLLKSLSELYLGNNPGLRELPPELGQLGNLWQLDTEDLTISNVPAEIQKEGPKAMLSYLRAQLRKAEKCKLMKMIIVGPPRQGKSTLLEILQTGRAPQVVHGEATIRTTKWELQRPAGSRAKVESVEFNVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVANLQFWLLNIEAKAPNAVVLVVGTHLDLIEAKFRVERIATLRAYVLALCRSPSGSRATGFPDITFKHLHEISCKSLEGQEGLRQLIFHVTCSMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEQQRRSRDDDVQYLTDRQLEQLVEQTPDNDIKDYEDLQSAISFLIETGTLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRMLLVGTGFTQQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENKKNTKSRNRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGMKIVCQSEVRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCETAWAQHTDPSEKSEDVQYFDMEDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHSEDEGSVLGQGGSGTVIYRARYQGQPVAVKRFHIKKFKNFANVPADTMLRHLRATDAMKNFSEFRQEASMLHALQHPCIVALIGISIHPLCFALELAPLSSLNTVLSENARDSSFIPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDVKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGHHQLQIAKKLSKGIRPVLGQPEEVQFRRLQALMMECWDTKPEKRPLALSVVSQMKDPTFATFMYELCCGKQTAFFSSQGQEYTVVFWDGKEESRNYTVVNTEKGLMEVQRMCCPGMKVSCQLQVQRSLWTATEDQKIYIYTLKGMCPLNTPQQALDTPAVVTCFLAVPVIKKNSYLVLAGLADGLVAVFPVVRGTPKDSCSYLCSHTANRSKFSIADEDARQNPYPVKAMEVVNSGSEVWYSNGPGLLVIDCASLEICRRLEPYMAPSMVTSVVCSSEGRGEEVVWCLDDKANSLVMYHSTTYQLCARYFCGVPSPLRDMFPVRPLDTEPPAASHTANPKVPEGDSIADVSIMYSEELGTQILIHQESLTDYCSMSSYSSSPPRQAARSPSSLPSSPASSSSVPFSTDCEDSDMLHTPGAASDRSEHDLTPMDGETFSQHLQAVKILAVRDLIWVPRRGGDVIVIGLEKDSGAQRGRVIAVLKARELTPHGVLVDAAVVAKDTVVCTFENENTEWCLAVWRGWGAREFDIFYQSYEELGRLEACTRKRR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU66250
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please insert S405E mutation into DU30382
• Low Temp: No