cDNA Clone mKATE2

• Synonyms: PELI3, Pellino3
• Plasmid: pCMV5 HA PELLINO3 IRES2 mKATE2
• Parent Plasmid: pCMV5 HA-1
• Expressed: HA-PELLINO3 + mKATE2
• Genbank: NM_145065.3
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGTGCTGGAAGGAAACCCTGAAGTGGGGTCCCCCCGAACCTCAGACCTCCAGCACCGGGGGAACAAGGGCTCTTGCGTTCTCTCCTCTCCCGGTGAAGATGCGCAGCCAGGCGAGGAGCCCATCAAGTATGGTGAACTCATCGTCCTGGGATGCTGTGAGGAAGGAGGTGAGGAAACCGAGGCTCAGAGAGGGGAAGTGACTGGCCCAAGGGCACACAGCTGCTACAATGGTTGTCTGGCAAGTGGGGACAAGGGCCGCCGGCGAAGCCGCCTGGCACTGAGCCGCCGGTCGCACGCCAACGGGGTGAAGCCAGACGTCATGCACCACATCTCCACGCCGCTCGTCTCCAAGGCACTGAGTAACCGTGGTCAGCACAGCATCTCGTATACACTGTCCCGGAGCCACTCGGTCATAGTGGAGTATACACATGATAGCGACACAGACATGTTCCAGATTGGCCGCTCCACAGAGAACATGATTGACTTCGTGGTAACAGACACGTCCCCTGGAGGAGGGGCTGCCGAGGGCCCTTCTGCCCAGAGCACCATCTCCCGCTATGCCTGCCGCATCCTCTGTGACCGCCGGCCACCCTATACTGCCCGCATCTATGCCGCTGGCTTCGACGCCTCTAGCAACATCTTCCTTGGAGAGCGAGCGGCCAAATGGCGGACCCCAGATGGCCTGATGGATGGACTGACCACCAATGGAGTCCTGGTGATGCACCCGGCAGGCGGCTTCTCCGAGGACTCAGCCCCGGGTGTCTGGCGGGAGATCTCGGTCTGTGGGAATGTGTACACATTGCGGGACAGCCGCTCAGCCCAGCAGCGGGGCAAGCTGGTAGGTGGCCCGCTCCATTCCCCACCCCTATCCTTGCCAGGCCCTCACAAGCTGCCCATCCCCCTGCCCAAGCCAGGAGATGATCTCCAGTCCTTCTGCCCCAGGGAGCCCCAACAGGCTCCATATCAGGGAATCCCAGGCCCAGGGGGTTCTTAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMVLEGNPEVGSPRTSDLQHRGNKGSCVLSSPGEDAQPGEEPIKYGELIVLGCCEEGGEETEAQRGEVTGPRAHSCYNGCLASGDKGRR
RSRLALSRRSHANGVKPDVMHHISTPLVSKALSNRGQHSISYTLSRSHSVIVEYTHDSDTDMFQIGRSTENMIDFVVTDTSPGGGAAEGPSAQSTISRYA
CRILCDRRPPYTARIYAAGFDASSNIFLGERAAKWRTPDGLMDGLTTNGVLVMHPAGGFSEDSAPGVWREISVCGNVYTLRDSRSAQQRGKLVGGPLHSP
PLSLPGPHKLPIPLPKPGDDLQSFCPREPQQAPYQGIPGPGGS*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5 HA PELLINO3 IRES2 mKATE276.47 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU69096
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Control construct for FACS with mKATE2
• Low Temp: No