SARS CoV-2 Nsp1-Nsp2

• Plasmid: pET15D 8His GST 3C Nsp1-Nsp2
• Parent Plasmid: pET15D 8His GST
• Expressed: 8His-GST-3C-Nsp1-Nsp2
• Genbank: QHD43415.1
• Tag: GST
• Tag: 6His
• Species: SARS-CoV-2

Sequence of Insert

GGATCCATGGAAAGCCTGGTGCCGGGCTTTAACGAAAAAACCCATGTGCAGCTGAGCCTGCCGGTGCTGCAGGTGCGCGATGTGCTGGTGCGCGGCTTTGGCGATAGCGTGGAAGAAGTGCTGAGCGAAGCGCGCCAGCATCTGAAAGATGGCACCTGCGGCCTGGTGGAAGTGGAAAAAGGCGTGCTGCCGCAGCTGGAACAGCCGTATGTGTTTATTAAACGCAGCGATGCGCGCACCGCGCCGCATGGCCATGTGATGGTGGAACTGGTGGCGGAACTGGAAGGCATTCAGTATGGCCGCAGCGGCGAAACCCTGGGCGTGCTGGTGCCGCATGTGGGCGAAATTCCGGTGGCGTATCGCAAAGTGCTGCTGCGCAAAAACGGCAACAAAGGCGCGGGCGGCCATAGCTATGGCGCGGATCTGAAAAGCTTTGATCTGGGCGATGAACTGGGCACCGATCCGTATGAAGATTTTCAGGAAAACTGGAACACCAAACATAGCAGCGGCGTGACCCGCGAACTGATGCGCGAACTGAACGGCGGCGCGTATACCCGCTATGTGGATAACAACTTTTGCGGCCCGGATGGCTATCCGCTGGAATGCATTAAAGATCTGCTGGCGCGCGCGGGCAAAGCGAGCTGCACCCTGAGCGAACAGCTGGATTTTATTGATACCAAACGCGGCGTGTATTGCTGCCGCGAACATGAACATGAAATTGCGTGGTATACCGAACGCAGCGAAAAAAGCTATGAACTGCAGACCCCGTTTGAAATTAAACTGGCGAAAAAATTTGATACCTTTAACGGCGAATGCCCGAACTTTGTGTTTCCGCTGAACAGCATTATTAAAACCATTCAGCCGCGCGTGGAAAAAAAAAAACTGGATGGCTTTATGGGCCGCATTCGCAGCGTGTATCCGGTGGCGAGCCCGAACGAATGCAACCAGATGTGCCTGAGCACCCTGATGAAATGCGATCATTGCGGCGAAACCAGCTGGCAGACCGGCGATTTTGTGAAAGCGACCTGCGAATTTTGCGGCACCGAAAACCTGACCAAAGAAGGCGCGACCACCTGCGGCTATCTGCCGCAGAACGCGGTGGTGAAAATTTATTGCCCGGCGTGCCATAACAGCGAAGTGGGCCCGGAACATAGCCTGGCGGAATATCATAACGAAAGCGGCCTGAAAACCATTCTGCGCAAAGGtGGaCGCACCATTGCGTTTGGCGGCTGCGTGTTTAGCTATGTGGGCTGCCATAACAAATGCGCGTATTGGGTGCCGCGCGCGAGCGCGAACATTGGCTGCAACCATACCGGCGTGGTGGGCGAAGGCAGCGAAGGCCTGAACGATAACCTGCTGGAAATTCTGCAGAAAGAAAAAGTGAACATTAACATTGTGGGCGATTTTAAACTGAACGAAGAAATTGCGATTATTCTGGCGAGCTTTAGCGCGAGCACCAGCGCGTTTGTGGAAACCGTGAAAGGCCTGGATTATAAAGCGTTTAAACAGATTGTGGAAAGCTGCGGCAACTTTAAAGTGACCAAAGGCAAAGCGAAAAAAGGCGCGTGGAACATTGGCGAACAGAAAAGCATTCTGAGCCCGCTGTATGCGTTTGCGAGCGAAGCGGCGCGCGTGGTGCGCAGCATTTTTAGCCGCACCCTGGAAACCGCGCAGAACAGCGTGCGCGTGCTGCAGAAAGCGGCGATTACCATTCTGGATGGCATTAGCCAGTATAGCCTGCGCCTGATTGATGCGATGATGTTTACCAGCGATCTGGCGACCAACAACCTGGTGGTGATGGCGTATATTACCGGCGGCGTGGTGCAGCTGACCAGCCAGTGGCTGACCAACATTTTTGGCACCGTGTATGAAAAACTGAAACCGGTGCTGGATTGGCTGGAAGAAAAATTTAAAGAAGGCGTGGAATTTCTGCGCGATGGCTGGGAAATTGTGAAATTTATTAGCACCTGCGCGTGCGAAATTGTGGGCGGCCAGATTGTGACCTGCGCGAAAGAAATTAAAGAAAGCGTGCAGACCTTTTTTAAACTGGTGAACAAATTTCTGGCGCTGTGCGCGGATAGCATTATTATTGGCGGCGCGAAACTGAAAGCGCTGAACCTGGGCGAAACCTTTGTGACCCATAGCAAAGGCCTGTATCGCAAATGCGTGAAAAGCCGCGAAGAAACCGGCCTGCTGATGCCGCTGAAAGCGCCGAAAGAAATTATTTTTCTGGAAGGCGAAACCCTGCCGACCGAAGTGCTGACCGAAGAAGTGGTGCTGAAAACCGGCGATCTGCAGCCGCTGGAACAGCCGACCAGCGAAGCGGTGGAAGCGCCGCTGGTGGGCACCCCGGTGTGCATTAACGGCCTGATGCTGCTGGAAATTAAAGATACCGAAAAATATTGCGCGCTGGCGCCGAACATGATGGTGACCAACAACACCTTTACCCTGAAAGGCGGCtgaGCGGCCGC

Amino Acid Sequence

MGHHHHHHHHSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAE
ISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDK
YLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGV
LPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQEN
WNTKHSSGVTRELMRELNGGAYTRYVDNNFCGPDGYPLECIKDLLARAGKASCTLSEQLDFIDTKRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLA
KKFDTFNGECPNFVFPLNSIIKTIQPRVEKKKLDGFMGRIRSVYPVASPNECNQMCLSTLMKCDHCGETSWQTGDFVKATCEFCGTENLTKEGATTCGYL
PQNAVVKIYCPACHNSEVGPEHSLAEYHNESGLKTILRKGGRTIAFGGCVFSYVGCHNKCAYWVPRASANIGCNHTGVVGEGSEGLNDNLLEILQKEKVN
INIVGDFKLNEEIAIILASFSASTSAFVETVKGLDYKAFKQIVESCGNFKVTKGKAKKGAWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNSVR
VLQKAAITILDGISQYSLRLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNIFGTVYEKLKPVLDWLEEKFKEGVEFLRDGWEIVKFISTCACEIV
GGQIVTCAKEIKESVQTFFKLVNKFLALCADSIIIGGAKLKALNLGETFVTHSKGLYRKCVKSREETGLLMPLKAPKEIIFLEGETLPTEVLTEEVVLKT
GDLQPLEQPTSEAVEAPLVGTPVCINGLMLLEIKDTEKYCALAPNMMVTNNTFTLKGG*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET15D 8His GST 3C Nsp1-Nsp2749.86 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU70079
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee