MERS Coronavirus S protein

• Plasmid: pGEX6P1 MERS CoV S protein (Custom)
• Parent Plasmid: pGEX6P1
• Expressed: GST 3C MERS CoV S protein
• Genbank: MG912608.1
• Tag: GST
• Species: MERS-CoV

Sequence of Insert

GGATCCATGATTCATAGCGTGTTTCTGCTGATGTTTCTGCTGACCCCGACCGAAAGCTATGTGGATGTGGGCCCGGATAGCGTGAAAAGCGCGTGCATTGAAGTGGATATTCAGCAGACCTTTTTTGATAAAACCTGGCCGCGCCCGATTGATGTGAGCAAAGCGGATGGCATTATTTATCCGCAGGGCCGCACCTATAGCAACATTACCATTACCTATCAGGGCCTGTTTCCGTATCAGGGCGATCATGGCGATATGTATGTGTATAGCGCGGGCCATGCGACCGGCACCACCCCGCAGAAACTGTTTGTGGCGAACTATAGCCAGGATGTGAAACAGTTTGCGAACGGCTTTGTGGTGCGCATTGGCGCGGCGGCGAACAGCACCGGCACCGTGATTATTAGCCCGAGCACCAGCGCGACCATTCGCAAAATTTATCCGGCGTTTATGCTGGGCAGCAGCGTGGGCAACTTTAGCGATGGCAAAATGGGCCGCTTTTTTAACCATACCCTGGTGCTGCTGCCGGATGGCTGCGGCACCCTGCTGCGCGCGTTTTATTGCATTCTGGAACCGCGCAGCGGCAACCATTGCCCGGCGGGCAACAGCTATACCAGCTTTGCGACCTATCATACCCCGGCGACCGATTGCAGCGATGGCAACTATAACCGCAACGCGAGCCTGAACAGCTTTAAAGAATATTTTAACCTGCGCAACTGCACCTTTATGTATACCTATAACATTACCGAAGATGAAATTCTGGAATGGTTTGGCATTACCCAGACCGCGCAGGGCGTGCATCTGTTTAGCAGCCGCTATGTGGATCTGTATGGCGGCAACATGTTTCAGTTTGCGACCCTGCCGGTGTATGATACCATTAAATATTATAGCATTATTCCGCATAGCATTCGCAGCATTCAGAGCGATCGCAAAGCGTGGGCGGCGTTTTATGTGTATAAACTGCAGCCGCTGACCTTTCTGCTGGATTTTAGCGTGGATGGCTATATTCGCCGCGCGATTGATTGCGGCTTTAACGATCTGAGCCAGCTGCATTGCAGCTATGAAAGCTTTGATGTGGAAAGCGGCGTGTATAGCGTGAGCAGCTTTGAAGCGAAACCGAGCGGCAGCGTGGTGGAACAGGCGGAAGGCGTGGAATGCGATTTTAGCCCGCTGCTGAGCGGCACCCCGCCGCAGGTGTATAACTTTAAACGCCTGGTGTTTACCAACTGCAACTATAACCTGACCAAACTGCTGAGCCTGTTTAGCGTGAACGATTTTACCTGCAGCCAGATTAGCCCGGCGGCGATTGCGAGCAACTGCTATAGCAGCCTGATTCTGGATTATTTTAGCTATCCGCTGAGCATGAAAAGCGATCTGAGCGTGAGCAGCGCGGGCCCGATTAGCCAGTTTAACTATAAACAGAGCTTTAGCAACCCGACCTGCCTGATTCTGGCGACCGTGCCGCATAACCTGACCACCATTACCAAACCGCTGAAATATAGCTATATTAACAAATGCAGCCGCCTGCTGAGCGATGATCGCACCGAAGTGCCGCAGCTGGTGAACGCGAACCAGTATAGCCCGTGCGTGAGCATTGTGCCGAGCACCGTGTGGGAAGATGGCGATTATTATCGCAAACAGCTGAGCCCGCTGGAAGGCGGCGGCTGGCTGGTGGCGAGCGGCAGCACCGTGGCGATGACCGAACAGCTGCAGATGGGCTTTGGCATTACCGTGCAGTATGGCACCGATACCAACAGCGTGTGCCCGAAACTGGAATTTGCGAACGATACCAAAATTGCGAGCCAGCTGGGCAACTGCGTGGAATATAGCCTGTATGGCGTGAGCGGtagaGGCGTGTTTCAGAACTGCACCGCGGTGGGCGTGCGCCAGCAGCGCTTTGTGTATGATGCGTATCAGAACCTGGTGGGCTATTATAGCGATGATGGCAACTATTATTGCCTGCGCGCGTGCGTGAGCGTGCCGGTGAGCGTGATTTATGATAAAGAAACCAAAACCCATGCGACCCTGTTTGGCAGCGTGGCGTGCGAACATATTAGCAGCACCATGAGCCAGTATAGCCGCAGCACCCGCAGCATGCTGAAACGCCGCGATAGCACCTATGGCCCGCTGCAGACCCCGGTGGGCTGCGTGCTGGGCCTGGTGAACAGCAGCCTGTTTGTGGAAGATTGCAAACTGCCGCTGGGCCAGAGCCTGTGCGCGCTGCCGGATACCCCGAGCACCCTGACCCCGCGCAGCGTGCGCAGCGTGCCGGGCGAAATGCGCCTGGCGAGCATTGCGTTTAACCATCCGATTCAGGTGGATCAGCTGAACAGCAGCTATTTTAAACTGAGCATTCCGACCAACTTTAGCTTTGGCGTGACCCAGGAATATATTCAGACCACCATTCAGAAAGTGACCGTGGATTGCAAACAGTATGTGTGCAACGGCTTTCAGAAATGCGAACAGCTGCTGCGCGAATATGGCCAGTTTTGCAGCAAAATTAACCAGGCGCTGCATGGCGCGAACCTGCGCCAGGATGATAGCGTGCGCAACCTGTTTGCGAGCGTGAAAAGCAGCCAGAGCAGCCCGATTATTCCGGGCTTTGGCGGCGATTTTAACCTGACCCTGCTGGAACCGGTGAGCATTAGCACCGGCAGCCGCAGCGCGCGCAGCGCGATTGAAGATCTGCTGTTTGATAAAGTGACCATTGCGGAcCCGGGCTATATGCAGGGCTATGATGATTGCATGCAGCAGGGCCCGGCGAGCGCGCGCGATCTGATTTGCGCGCAGTATGTGGCGGGCTATAAAGTGCTGCCGCCGCTGATGGATGTGAACATGGAAGCGGCGTATACCAGCAGCCTGCTGGGCAGCATTGCGGGCGTGGGCTGGACCGCGGGCCTGAGCAGCTTTGCGGCGATTCCGTTTGCGCAGAGCATTTTTTATCGCCTGAACGGCGTGGGCATTACCCAGCAGGTGCTGAGCGAAAACCAGAAACTGATTGCGAACAAATTTAACCAGGCGCTGGGCGCGATGCAGACCGGCTTTACCACCACCAACGAAGCGTTTCGCAAAGTGCAGGATGCGGTGAACAACAACGCGCAGGCGCTGAGCAAACTGGCGAGCGAACTGAGCAACACCTTTGGCGCGATTAGCGCGAGCATTGGCGATATTATTCAGCGCCTGGATGTGCTGGAACAGGATGCGCAGATTGATCGCCTGATTAACGGCCGCCTGACCACCCTGAACGCGTTTGTGGCGCAGCAGCTGGTGCGCAGCGAAAGCGCGGCGCTGAGCGCGCAGCTGGCGAAAGATAAAGTGAACGAATGCGTGAAAGCGCAGAGCAAACGCAGCGGCTTTTGCGGCCAGGGCACCCATATTGTGAGCTTTGTGGTGAACGCGCCGAACGGCCTGTATTTTATGCATGTGGGCTATTATCCGAGCAACCATATTGAAGTGGTGAGCGCGTATGGCCTGTGCGATGCGGCGAACCCGACCAACTGCATTGCGCCGGTGAACGGCTATTTTATTAAAACCAACAACACCCGCATTGTGGATGAATGGAGCTATACCGGCAGCAGCTTTTATGCGCCGGAACCGATTACCAGCCTGAACACCAAATATGTGGCGCCGCAGGTGACCTATCAGAACATTAGCACCAACCTGCCGCCGCCGCTGCTGGGCAACAGCACCGGCATTGATTTTCAGGATGAACTGGATGAATTTTTTAAAAACGTGAGCACCAGCATTCCGAACTTTGGCAGCCTGACCCAGATTAACACCACCCTGCTGGATCTGACCTATGAAATGCTGAGCCTGCAGCAGGTGGTGAAAGCGCTGAACGAAAGCTATATTGATCTGAAAGAACTGGGCAACTATACCTATTATAACAAATGGCCGTGGTATATTTGGCTGGGCTTTATTGCGGGCCTGGTGGCGCTGGCGCTGTGCGTGTTTTTTATTCTGTGCTGCACCGGCTGCGGCACCAACTGCATGGGCAAACTGAAATGCAACCGCTGCTGCGATCGCTATGAAGAATATGATCTGGAACCGCATAAAGTGCATGTGCATtgaGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMIHSVFLLMFLLTPTESYVDVGPDSVKSACIEVDIQQTFFDKTWPRPIDVSKADGIIYPQGRTYSNITITYQGLFPYQGDHGDMYVYSAGHATGTTPQKLFVANYSQDVKQFANGFVVRIGAAANSTGTVIISPSTSATIRKIYPAFMLGSSVGNFSDGKMGRFFNHTLVLLPDGCGTLLRAFYCILEPRSGNHCPAGNSYTSFATYHTPATDCSDGNYNRNASLNSFKEYFNLRNCTFMYTYNITEDEILEWFGITQTAQGVHLFSSRYVDLYGGNMFQFATLPVYDTIKYYSIIPHSIRSIQSDRKAWAAFYVYKLQPLTFLLDFSVDGYIRRAIDCGFNDLSQLHCSYESFDVESGVYSVSSFEAKPSGSVVEQAEGVECDFSPLLSGTPPQVYNFKRLVFTNCNYNLTKLLSLFSVNDFTCSQISPAAIASNCYSSLILDYFSYPLSMKSDLSVSSAGPISQFNYKQSFSNPTCLILATVPHNLTTITKPLKYSYINKCSRLLSDDRTEVPQLVNANQYSPCVSIVPSTVWEDGDYYRKQLSPLEGGGWLVASGSTVAMTEQLQMGFGITVQYGTDTNSVCPKLEFANDTKIASQLGNCVEYSLYGVSGRGVFQNCTAVGVRQQRFVYDAYQNLVGYYSDDGNYYCLRACVSVPVSVIYDKETKTHATLFGSVACEHISSTMSQYSRSTRSMLKRRDSTYGPLQTPVGCVLGLVNSSLFVEDCKLPLGQSLCALPDTPSTLTPRSVRSVPGEMRLASIAFNHPIQVDQLNSSYFKLSIPTNFSFGVTQEYIQTTIQKVTVDCKQYVCNGFQKCEQLLREYGQFCSKINQALHGANLRQDDSVRNLFASVKSSQSSPIIPGFGGDFNLTLLEPVSISTGSRSARSAIEDLLFDKVTIADPGYMQGYDDCMQQGPASARDLICAQYVAGYKVLPPLMDVNMEAAYTSSLLGSIAGVGWTAGLSSFAAIPFAQSIFYRLNGVGITQQVLSENQKLIANKFNQALGAMQTGFTTTNEAFRKVQDAVNNNAQALSKLASELSNTFGAISASIGDIIQRLDVLEQDAQIDRLINGRLTTLNAFVAQQLVRSESAALSAQLAKDKVNECVKAQSKRSGFCGQGTHIVSFVVNAPNGLYFMHVGYYPSNHIEVVSAYGLCDAANPTNCIAPVNGYFIKTNNTRIVDEWSYTGSSFYAPEPITSLNTKYVAPQVTYQNISTNLPPPLLGNSTGIDFQDELDEFFKNVSTSIPNFGSLTQINTTLLDLTYEMLSLQQVVKALNESYIDLKELGNYTYYNKWPWYIWLGFIAGLVALALCVFFILCCTGCGTNCMGKLKCNRCCDRYEEYDLEPHKVHVH*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P1 MERS CoV S protein (Custom)140.37 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU67745
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee