MINDY4

• Synonyms: AQP-1, AQP1, C7orf67, CHIP28, FAM188B
• Plasmid: pET DUET 6HIS TEV GST 3C AVI MINDY4
• Parent Plasmid: pET DUET2
• Expressed: 6HIS TEV GST 3C AVI MINDY4
• Genbank: NM_032222.3
• Tag: GST
• Tag: 6His
• Tag: AVI
• Species: Human

Sequence of Insert

GGATCTATGGACAGCCTCTTCGTGGAGGAGGTGGCCGCCTCCTTGGTCAGGGAGTTCCTCAGCAGAAAGGGCTTAAAGAAGACATGTGTGACCATGGACCAGGAACGCCCACGCTCTGACCTCAGCATAAACAACAGAAATGATCTTCGAAAGGTTTTGCATCTTGAATTTCTCTATAAGGAGAACAAGGCAAAGGAAAATCCTCTAAAAACAAGCCTTGAACTCATCACCAGATACTTTCTGGATCACTTTGGAAATACGGCTAACAATTTCACTCAAGATACCCCAATCCCTGCACTCTCAGTTCCAAAGAAAAATAACAAAGTGCCATCAAGATGCTCAGAGACTACACTGGTAAATATATATGACCTTTCAGATGAAGATGCAGGATGGAGAACATCATTGTCAGAAACAAGCAAAGCCAGACATGACAATCTTGATGGAGATGTACTTGGTAATTTTGTATCATCTAAAAGGCCCCCGCACAAAAGTAAGCCCATGCAGACGGTCCCGGGTGAAACTCCTGTGTTGACTTCTGCATGGGAGAAGATAGACAAGCTTCACTCGGAGCCTTCCTTGGATGTGAAGAGGATGGGAGAGAATTCCAGGCCAAAGTCTGGTCTGATTGTGCGAGGCATGATGTCTGGGCCCATCGCCAGCTCCCCACAGGATTCTTTTCACAGACACTATCTGAGACGGTCCTCACCGTCAAGCAGCTCCACCCAACCCCAAGAAGAGAGCCGGAAGGTCCCTGAGCTCTTTGTCTGCACCCAACAGGACATTCTGGCTTCGAGCAACAGCTCCCCCTCCAGGACCTCCCTGGGTCAGCTTAGTGAACTGACCGTAGAAAGGCAGAAAACCACTGCCAGCAGCCCTCCCCATCTGCCCAGCAAACGGCTGCCCCCATGGGACAGGGCCAGGCCGAGGGATCCCTCCGAGGACACCCCAGCAGTGGACGGCAGCACAGACACGGACAGGATGCCCTTGAAGCTCTACTTGCCTGGTGGTAATTCCAGGATGACCCAGGAGAGGCTGGAAAGAGCGTTCAAACGGCAGGGCAGCCAGCCCGCACCTGTCAGGAAAAATCAGTTGCTGCCGTCTGACAAGGTGGATGGTGAGCTGGGTGCCCTGCGGCTCGAGGATGTGGAGGATGAGTTGATAAGGGAAGAGGTCATCCTGTCGCCAGTCCCATCAGTGCTCAAGTTGCAGACAGCATCAAAACCAATTGACCTCTCAGTAGCAAAGGAAATAAAGACCCTTCTGTTTGGTTCCAGCTTTTGCTGTTTCAATGAAGAATGGAAACTTCAGAGTTTTTCCTTTAGTAACACAGCCTCATTAAAATACGGCATAGTGCAGAACAAGGGTGGTCCTTGCGGAGTCCTGGCAGCTGTCCAAGGCTGTGTCCTACAGAAACTCCTGTTTGAAGGAGATAGCAAAGCCGACTGTGCTCAGGGACTGCAGCCTTCAGATGCCCACCGGACCCGCTGCCTCGTCCTGGCCCTCGCAGACATTGTGTGGCGGGCAGGGGGCCGAGAGAGAGCCGTTGTTGCACTGGCTTCGAGAACACAGCAGTTCAGTCCAACAGGGAAATACAAAGCAGATGGAGTCTTAGAAACACTTACGCTTCACAGTTTGACCTGCTATGAGGACCTGGTGACTTTTCTTCAACAAAGCATTCATCAGTTTGAAGTGGGCCCCTATGGCTGCATCCTGCTCACCCTTTCTGCCATCCTGTCCAGGTCTACAGAGCTCATCCGCCAGGACTTTGATGTCCCCACCAGCCACCTGATTGGAGCACATGGCTACTGTACACAGGAACTTGTCAATCTGCTCCTGACTGGGAAAGCTGTGTCCAACGTTTTCAACGATGTGGTTGAGCTGGATTCTGGGGATGGGAACATCACACTTCTCAGAGGCATTGCTGCACGCAGTGATATTGGCTTCTTATCTCTCTTTGAGCATTACAACATGTGCCAGGTTGGCTGCTTCCTGAAGACCCCGAGGTTCCCCATCTGGGTGGTTTGCAGTGAGAGCCACTTCAGCATCCTCTTTAGCCTGCAGCCGGGGCTCCTGCGTGACTGGAGGACTGAGAGGCTCTTTGACTTGTACTACTACGATGGCCTGGCCAACCAGCAGGAGCAGATCCGGCTGACCATTGACACCACCCAAACCATCTCTGAGGACACAGACAACGACCTTGTCCCACCCCTCGAGCTCTGCATCAGAACCAAGTGGAAGGGGGCATCAGTGAACTGGAACGGCTCAGACCCCATCCTGTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHPGSENLYFQMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGLNDIFEAQKIEWHEGSMDSLFVEEVAASLVREFLSRKGLKKTCVTMDQERPRSDLSINNRNDLRKVLHLEFLYKENKAKENPLKTSLELITRYFLDHFGNTANNFTQDTPIPALSVPKKNNKVPSRCSETTLVNIYDLSDEDAGWRTSLSETSKARHDNLDGDVLGNFVSSKRPPHKSKPMQTVPGETPVLTSAWEKIDKLHSEPSLDVKRMGENSRPKSGLIVRGMMSGPIASSPQDSFHRHYLRRSSPSSSSTQPQEESRKVPELFVCTQQDILASSNSSPSRTSLGQLSELTVERQKTTASSPPHLPSKRLPPWDRARPRDPSEDTPAVDGSTDTDRMPLKLYLPGGNSRMTQERLERAFKRQGSQPAPVRKNQLLPSDKVDGELGALRLEDVEDELIREEVILSPVPSVLKLQTASKPIDLSVAKEIKTLLFGSSFCCFNEEWKLQSFSFSNTASLKYGIVQNKGGPCGVLAAVQGCVLQKLLFEGDSKADCAQGLQPSDAHRTRCLVLALADIVWRAGGRERAVVALASRTQQFSPTGKYKADGVLETLTLHSLTCYEDLVTFLQQSIHQFEVGPYGCILLTLSAILSRSTELIRQDFDVPTSHLIGAHGYCTQELVNLLLTGKAVSNVFNDVVELDSGDGNITLLRGIAARSDIGFLSLFEHYNMCQVGCFLKTPRFPIWVVCSESHFSILFSLQPGLLRDWRTERLFDLYYYDGLANQQEQIRLTIDTTQTISEDTDNDLVPPLELCIRTKWKGASVNWNGSDPIL*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET DUET 6HIS TEV GST 3C AVI MINDY4130.64 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU68514
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee