VPS35

• Synonyms: MEM3, PARK17
• Plasmid: pBabeD puro VPS35 R312C
• Parent Plasmid: pBabeD puro
• Expressed: VPS35 R312C
• Genbank: NM_018206
• Species: Human

Sequence of Insert

ggatccATGCCTACAACACAGCAGTCCCCTCAGGATGAGCAGGAAAAGCT
CTTGGATGAAGCCATACAGGCTGTGAAGGTCCAGTCATTCCAAATGAAGA
GATGCCTGGACAAAAACAAGCTTATGGATGCTCTAAAACATGCTTCTAAT
ATGCTTGGTGAACTCCGGACTTCTATGTTATCACCAAAGAGTTACTATGA
ACTTTATATGGCCATTTCTGATGAACTGCACTACTTGGAGGTCTACCTGA
CAGATGAGTTTGCTAAAGGAAGGAAAGTGGCAGATCTCTACGAACTTGTA
CAGTATGCTGGAAACATTATCCCAAGGCTTTACCTTTTGATCACAGTTGG
AGTTGTATATGTCAAGTCATTTCCTCAGTCCAGGAAGGATATTTTGAAAG
ATTTGGTAGAAATGTGCCGTGGTGTGCAACATCCCTTGAGGGGTCTGTTT
CTTCGAAATTACCTTCTTCAGTGTACCAGAAATATCTTACCTGATGAAGG
AGAGCCAACAGATGAAGAAACAACTGGTGACATCAGTGATTCCATGGATT
TTGTACTGCTCAACTTTGCAGAAATGAACAAGCTCTGGGTGCGAATGCAG
CATCAGGGACATAGCCGAGATAGAGAAAAAAGAGAACGAGAAAGACAAGA
ACTGAGAATTTTAGTGGGAACAAATTTGGTGCGCCTCAGTCAGTTGGAAG
GTGTAAATGTGGAACGTTACAAACAGATTGTTTTGACTGGCATATTGGAG
CAAGTTGTAAACTGTAGGGATGCTTTGGCTCAAGAATATCTCATGGAGTG
TATTATTCAGGTTTTCCCTGATGAATTTCACCTCCAGACTTTGAATCCTT
TTCTTCGGGCCTGTGCTGAGTTACACCAGAATGTAAATGTGAAGAACATA
ATCATTGCTTTAATTGATAGATTAGCTTTATTTGCTCACtGTGAAGATGG
ACCTGGAATCCCAGCGGATATTAAACTTTTTGATATATTTTCACAGCAGG
TGGCTACAGTGATACAGTCTAGACAAGACATGCCTTCAGAGGATGTTGTA
TCTTTACAAGTCTCTCTGATTAATCTTGCCATGAAATGTTACCCTGATCG
TGTGGACTATGTTGATAAAGTTCTAGAAACAACAGTGGAGATATTCAATA
AGCTCAACCTTGAACATATTGCTACCAGTAGTGCAGTTTCAAAGGAACTC
ACCAGACTTTTGAAAATACCAGTTGACACTTACAACAATATTTTAACAGT
CTTGAAATTAAAACATTTTCACCCACTCTTTGAGTACTTTGACTACGAGT
CCAGAAAGAGCATGAGTTGTTATGTGCTTAGTAATGTTCTGGATTATAAC
ACAGAAATTGTCTCTCAAGACCAGGTGGATTCCATAATGAATTTGGTATC
CACGTTGATTCAAGATCAGCCAGATCAACCTGTAGAAGACCCTGATCCAG
AAGATTTTGCTGATGAGCAGAGCCTTGTGGGCCGCTTCATTCATCTGCTG
CGCTCTGAGGACCCTGACCAGCAGTACTTGATTTTGAACACAGCACGAAA
ACATTTTGGAGCTGGTGGAAATCAGCGGATTCGCTTCACACTGCCACCTT
TGGTATTTGCAGCTTACCAGCTGGCTTTTCGATATAAAGAGAATTCTAAA
GTGGATGACAAATGGGAAAAGAAATGCCAGAAGATTTTTTCATTTGCCCA
CCAGACTATCAGTGCTTTGATCAAAGCAGAGCTGGCAGAATTGCCCTTAA
GACTTTTTCTTCAAGGAGCACTAGCTGCTGGGGAAATTGGTTTTGAAAAT
CATGAGACAGTCGCATATGAATTCATGTCCCAGGCATTTTCTCTGTATGA
AGATGAAATCAGCGATTCCAAAGCACAGCTAGCTGCCATCACCTTGATCA
TTGGCACTTTTGAAAGGATGAAGTGCTTCAGTGAAGAGAATCATGAACCT
CTGAGGACTCAGTGTGCCCTTGCTGCATCCAAACTTCTAAAGAAACCTGA
TCAGGGCCGAGCTGTGAGCACCTGTGCACATCTCTTCTGGTCTGGCAGAA
ACACGGACAAAAATGGGGAGGAGCTTCACGGAGGCAAGAGGGTAATGGAG
TGCCTAAAAAAAGCTCTAAAAATAGCAAATCAGTGCATGGACCCCTCTCT
ACAAGTGCAGCTTTTTATAGAAATTCTGAACAGATATATCTATTTTTATG
AAAAGGAAAATGATGCGGTAACAATTCAGGTTTTAAACCAGCTTATCCAA
AAGATTCGAGAAGACCTCCCGAATCTTGAATCCAGTGAAGAAACAGAGCA
GATTAACAAACATTTTCATAACACACTGGAGCATTTGCGCTTGCGGCGGG
AATCACCAGAATCCGAGGGGCCAATTTATGAAGGTCTCATCCTTTAAGCG
GCCGC

Amino Acid Sequence

MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNML
GELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQY
AGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLR
NYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQ
GHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQV
VNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIII
ALIDRLALFAHCEDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSL
QVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTR
LLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTE
IVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRS
EDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVD
DKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHE
TVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLR
TQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECL
KKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKI
REDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65393
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee