RNF25

• Plasmid: pGEX6P-3-RNF25 C316A
• Parent Plasmid: pGEX6P-3
• Expressed: GST-RNF25 C316A
• Genbank: NM_022453.3 → NP_071898.2
• Tag: GST
• Species: Human

Sequence of Insert

gtcgacATGGCGGCGTCTGCGTCTGCAGCTGCAGGGGAGGAGGACTGGGT
CCTTCCCTCTGAAGTTGAAGTATTGGAGTCCATCTATCTAGATGAACTAC
AGGTGATTAAAGGAAATGGCAGAACTTCACCATGGGAGATCTACATCACT
TTGCATCCTGCCACTGCAGAGGACCAGGATTCACAGTATGTCTGCTTCAC
TCTGGTGCTTCAGGTCCCAGCAGAGTATCCCCATGAGGTGCCACAGATCT
CTATCCGAAATCCCCGAGGACTTTCAGATGAACAGATCCACACGATCTTA
CAGGTGCTGGGCCACGTGGCCAAGGCTGGGCTGGGCACTGCCATGCTGTA
TGAACTCATTGAGAAAGGGAAGGAAATTCTCACAGATAACAACATCCCTC
ATGGCCAGTGTGTCATCTGCCTCTATGGTTTCCAGGAGAAGGAGGCCTTT
ACCAAAACACCCTGTTACCACTACTTCCACTGCCACTGCCTTGCTCGGTA
CATCCAGCACATGGAGCAAGAGCTGAAGGCACAAGGACAGGAGCAGGAAC
AGGAACGGCAGCATGCTACAACCAAACAGAAGGCAGTCGGTGTGCAGTGT
CCAGTGTGCAGAGAGCCCCTCGTGTATGATCTTGCCTCACTGAAAGCAGC
CCCTGAACCCCAACAGCCCATGGAGCTGTACCAGCCCAGTGCAGAGAGCT
TGCGCCAGCAAGAAGAACGCAAGCGGCTCTACCAGAGGCAGCAGGAGCGG
GGGGGAATCATTGACCTTGAGGCTGAGCGAAACCGATACTTCATCAGCCT
TCAGCAGCCTCCTGCCCCTGCGGAACCTGAGTCAGCTGTAGATGTCTCCA
AAGGATCCCAACCACCCAGCACCCTTGCAGCAGAACTATCCACCTCACCA
GCCGTCCAATCCACTTTGCCACCTCCTCTGCCTGTGGCGACCCAGCACAT
AGCTGAGAAGATTCCAGGGACCAGGTCAAATCAGCAAAGGTTGGGCGAAA
CCCAGAAAGCTATGCTAGATCCCCCCAAGCCCAGTCGAGGTCCCTGGCGA
CAGCCCGAACGGAGGCACCCAAAGGGAGGGGAGTGCCACGCCCCTAAAGG
TACCCGTGACACCCAGGAACTGCCACCTCCTGAGGGGCCCCTCAAGGAGC
CCATGGACCTAAAGCCAGAACCCCATAGCCAAGGAGTTGAAGGTCCTCCA
CAAGAGAAGGGGCCTGGCAGCTGGCAGGGGCCCCCACCCCGCAGGACTCG
GGACTGTGTTCGCTGGGAGCGCTCTAAAGGCCGGACACCCGGTTCTTCCT
ACCCTCGCCTGCCTCGGGGCCAGGGAGCATACCGGCCTGGTACTCGGAGG
GAGTCCCTGGGCCTGGAATCTAAGGATGGTTCCTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDMAASASAAAGEED
WVLPSEVEVLESIYLDELQVIKGNGRTSPWEIYITLHPATAEDQDSQYVC
FTLVLQVPAEYPHEVPQISIRNPRGLSDEQIHTILQVLGHVAKAGLGTAM
LYELIEKGKEILTDNNIPHGQCVICLYGFQEKEAFTKTPCYHYFHCHCLA
RYIQHMEQELKAQGQEQEQERQHATTKQKAVGVQCPVCREPLVYDLASLK
AAPEPQQPMELYQPSAESLRQQEERKRLYQRQQERGGIIDLEAERNRYFI
SLQQPPAPAEPESAVDVSKGSQPPSTLAAELSTSPAVQSTLPPPLPVATQ
HIAEKIPGTRSNQQRLGETQKAMLDPPKPSRGPWRQPERRHPKGGECHAP
KGTRDTQELPPPEGPLKEPMDLKPEPHSQGVEGPPQEKGPGSWQGPPPRR
TRDCVRWERSKGRTPGSSYPRLPRGQGAYRPGTRRESLGLESKDGS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU65190
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee