6His Halo-JOSD1

• Plasmid: pET28a 6HIS TEV HALO-JOSD1 C36A
• Parent Plasmid: pET28a 6His TEV Halo
• Expressed: 6HIS TEV HALO-JOSD1 C36A
• Genbank: NM_014876.5
• Tag: 6His
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCATGAGTTGTGTGCCATGGAAAGGAGACAAGGCCAAATCTGAATC
ATTGGAGCTGCCCCAGGCAGCACCCCCACAAATCTACCATGAGAAACAGC
GCAGGGAGCTTGCTGCCCTCCACGCCCTCAATAACGTCTTCCAGGACAGC
AATGCCTTCACCCGGGATACGCTGCAAGAGATTTTCCAGAGGTTGTCTCC
AAACACCATGGTGACACCTCACAAGAAGAGCATGCTGGGAAATGGCAACT
ACGATGTGAATGTCATTATGGCAGCACTTCAGACCAAAGGCTATGAAGCT
GTTTGGTGGGACAAGCGCAGGGATGTCGGTGTCATTGCCCTCACTAACGT
CATGGGCTTCATCATGAATCTGCCCTCCAGCCTATGCTGGGGTCCACTGA
AACTGCCCCTCAAAAGGCAGCACTGGATCTGTGTTCGAGAGGTGGGAGGG
GCCTACTACAACCTCGACTCCAAACTCAAGATGCCCGAGTGGATTGGAGG
CGAGAGCGAGCTCAGGAAGTTTCTAAAACATCATTTGCGAGGAAAGAACT
GTGAACTCCTGCTGGTGGTACCAGAAGAGGTAGAGGCTCATCAGAGTTGG
AGGACCGATGTGTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHENLYFQGGMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDG
TPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFD
DHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFI
RPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLT
EVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQS
PVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
GSEIARWLSTLEISGGSMSCVPWKGDKAKSESLELPQAAPPQIYHEKQRR
ELAALHALNNVFQDSNAFTRDTLQEIFQRLSPNTMVTPHKKSMLGNGNYD
VNVIMAALQTKGYEAVWWDKRRDVGVIALTNVMGFIMNLPSSLCWGPLKL
PLKRQHWICVREVGGAYYNLDSKLKMPEWIGGESELRKFLKHHLRGKNCE
LLLVVPEEVEAHQSWRTDV*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU29562
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee