TRIM63

• Plasmid: pRetroxTight puro TRIM63-GFP
• Parent Plasmid: pRetroX tight puro C-GFP
• Expressed: TRIM63-GFP
• Genbank: NM_032588.2 with one silent change
• Tag: GFP
• Species: Human

Sequence of Insert

aGATCTGCCACCATGGATTATAAGTCGAGCCTGATCCAGGATGGGAATCCCATGGAGAACTTGGAGAAGCAGCTGATCTGCCCTATCTGCCTGGAGATGTTTACCAAGCCAGTGGTCATCTTGCCGTGCCAGCACAACCTGTGCCGGAAGTGTGCCAATGACATCTTCCAGGCTGCAAATCCCTACTGGACCAGCCGGGGCAGCTCAGTGTCCATGTCTGGAGGCCGTTTCCGCTGCCCCACCTGCCGCCACGAGGTGATCATGGATCGTCACGGAGTGTACGGCCTGCAGAGGAACCTGCTGGTGGAGAACATCATCGACATCTACAAACAGGAGTGCTCCAGTCGGCCGCTGCAGAAGGGCAGTCACCCCATGTGCAAGGAGCACGAAGATGAGAAAATCAACATCTACTGTCTCACGTGTGAGGTGCCCACCTGCTCCATGTGCAAGGTGTTTGGGATCCACAAGGCCTGCGAGGTGGCCCCATTGCAGAGTGTCTTCCAGGGACAAAAGACTGAACTGAATAACTGTATCTCCATGCTGGTGGCGGGGAATGACCGTGTGCAGACCATCATCACTCAGCTGGAGGATTCCCGTCGAGTGACCAAGGAGAACAGTCACCAGGTAAAGGAAGAGCTGAGCCAGAAGTTTGACACGTTGTATGCCATCCTGGATGAGAAGAAAAGTGAGTTGCTGCAGCGGATCACGCAGGAGCAGGAGAAAAAGCTTAGCTTCATCGAGGCCCTCATCCAGCAGTACCAGGAGCAGCTGGACAAGTCCACAAAGCTGGTGGAAACTGCCATCCAGTCCCTGGACGAGCCTGGGGGAGCCACCTTCCTCTTGACTGCCAAGCAACTCATCAAAAGCATTGTGGAAGCTTCCAAGGGCTGCCAGCTGGGGAAGACAGAGCAGGGCTTTGAGAACATGGACTTCTTTACTTTGGATTTAGAGCACATAGCAGACGCCCTGAGAGCCATTGACTTTGGGACAGATGAGGAAGAGGAAGAATTCATTGAAGAAGAAGATCAGGAGGAGGAAGAGTCCACAGAAGGGAAGGAAGAAGGACACCAGACGCGT

Amino Acid Sequence

MDYKSSLIQDGNPMENLEKQLICPICLEMFTKPVVILPCQHNLCRKCANDIFQAANPYWTSRGSSVSMSGGRFRCPTCRHEVIMDRHGVYGLQRNLLVEN
IIDIYKQECSSRPLQKGSHPMCKEHEDEKINIYCLTCEVPTCSMCKVFGIHKACEVAPLQSVFQGQKTELNNCISMLVAGNDRVQTIITQLEDSRRVTKE
NSHQVKEELSQKFDTLYAILDEKKSELLQRITQEQEKKLSFIEALIQQYQEQLDKSTKLVETAIQSLDEPGGATFLLTAKQLIKSIVEASKGCQLGKTEQ
GFENMDFFTLDLEHIADALRAIDFGTDEEEEEFIEEEDQEEEESTEGKEEGHQTRMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYN
YNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pRetroxTight puro TRIM63-GFP794.15 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU63163
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee