TR-TUBE

• Plasmid: pcDNA5D frtTO HALO TR-TUBE Bdef c-HA
• Parent Plasmid: pcDNA5D frtTO HALO
• Expressed: HALO-3C-5GLY-TR-TUBE Bdef-5GLY-HA
• Tag: HA
• Tag: HALO

Sequence of Insert

GGATCCGGTGGCGGGGGTGGAGGGATTCTCAGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCGCATTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACGCCGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATTAATGCAGCTATTGAAGCCGCTGCCGGCTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCGCCTTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACGCCGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATTAATGCAGCTATTGAAGCAGCTGCCGGCTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGTAAATCCTCAGCTACAGAATCCAGAAGTCGCCTTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACGCGGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATTAATGCAGCTATTGAAGCGGCTGCCGGCTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCGCCTTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACGCCGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATTAATGCAGCTATTGAAGCAGCGGCTGGCTCCCAGCCATCAGGAGGTGGAGGATCGATCCCCGGAGGTGGAGGGGGTGAATTC

Amino Acid Sequence

MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEV
VLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDRE
PLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGLEV
LFQGPGSGGGGGGILRSGGGVNPQLQNPEVAFQQQLEQLSAMGFLNAEANLQALIATGGDINAAIEAAAGSQPSGGGGSGGGVNPQLQNPEVAFQQQLEQ
LSAMGFLNAEANLQALIATGGDINAAIEAAAGSQPSGGGGSGGVNPQLQNPEVAFQQQLEQLSAMGFLNAEANLQALIATGGDINAAIEAAAGSQPSGGG
GSGGGVNPQLQNPEVAFQQQLEQLSAMGFLNAEANLQALIATGGDINAAIEAAAGSQPSGGGGSIPGGGGGEFASLEKLAAAYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5D frtTO HALO TR-TUBE Bdef c-HA66.35 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU60416
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee