TR-TUBE

• Plasmid: pcDNA5D frtTO HALO TR-TUBE c-HA
• Parent Plasmid: pcDNA5D frtTO HALO
• Expressed: HALO-3C-5GLY-TR-TUBE-5GLY-HA
• Tag: HA
• Tag: HALO

Sequence of Insert

GGATCCGGTGGCGGGGGTGGAGGGATTCTCAGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCGCGTTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACGCGGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATTAATGCAGCTATTGAAGCGTTACTGGGCTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCGCGTTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACGCGGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATTAATGCAGCTATTGAAGCGTTACTGGGCTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGTAAATCCTCAGCTACAGAATCCAGAAGTCGCGTTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACGCGGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATTAATGCAGCTATTGAAGCGTTACTGGGCTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCGCGTTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACGCGGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATTAATGCAGCTATTGAAGCGTTACTGGGCTCCCAGCCATCAGGAGGTGGAGGATCGATCCCCGGAGGTGGAGGGGGTGAATTC

Amino Acid Sequence

MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEV
VLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDRE
PLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGLEV
LFQGPGSGGGGGGILRSGGGVNPQLQNPEVAFQQQLEQLSAMGFLNAEANLQALIATGGDINAAIEALLGSQPSGGGGSGGGVNPQLQNPEVAFQQQLEQ
LSAMGFLNAEANLQALIATGGDINAAIEALLGSQPSGGGGSGGVNPQLQNPEVAFQQQLEQLSAMGFLNAEANLQALIATGGDINAAIEALLGSQPSGGG
GSGGGVNPQLQNPEVAFQQQLEQLSAMGFLNAEANLQALIATGGDINAAIEALLGSQPSGGGGSIPGGGGGEFASLEKLAAAYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5D frtTO HALO TR-TUBE c-HA66.3 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU60415
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee