TR-TUBE

• Plasmid: pET28a 6His TEVb Halo TR-TUBE
• Parent Plasmid: pET28a 6His TEVb Halo
• Expressed: 6His TEVb Halo TR-TUBE
• Tag: 6His
• Tag: HALO

Sequence of Insert

ggatcCggtggcgggggtggaGGGATTCTCAGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCGCGTTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACGCGGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATTAATGCAGCTATTGAAGCGTTACTGGGCTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCGCGTTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACGCGGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATTAATGCAGCTATTGAAGCGTTACTGGGCTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGTAAATCCTCAGCTACAGAATCCAGAAGTCGCGTTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACGCGGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATTAATGCAGCTATTGAAGCGTTACTGGGCTCCCAGCCATCAGGAGGTGGAGGATCTGGAGGTGGAGTAAATCCTCAGCTACAGAATCCAGAAGTCGCGTTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACGCGGAAGCAAACTTGCAAGCTCTAATAGCAACAGGAGGTGATATTAATGCAGCTATTGAAGCGTTACTGGGCTCCCAGCCATCAGGAGGTGGAGGATCGATCCCCggaggtggagggggtGAATTC

Amino Acid Sequence

MGSSHHHHHHENLYFQMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDH
VRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEV
EMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGS
EIARWLSTLEISGGSGGGGGGILRSGGGVNPQLQNPEVAFQQQLEQLSAMGFLNAEANLQALIATGGDINAAIEALLGSQPSGGGGSGGGVNPQLQNPEV
AFQQQLEQLSAMGFLNAEANLQALIATGGDINAAIEALLGSQPSGGGGSGGVNPQLQNPEVAFQQQLEQLSAMGFLNAEANLQALIATGGDINAAIEALL
GSQPSGGGGSGGGVNPQLQNPEVAFQQQLEQLSAMGFLNAEANLQALIATGGDINAAIEALLGSQPSGGGGSIPGGGGGEFELRRQACGRTRAPPPPPLR
SGC*

• Vector Type: Bacterial
• Antibiotic: Kan
• Full Snapgene Map: pET28a 6His TEVb Halo TR-TUBE286.92 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU58810
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee