BAP1

• Plasmid: pET15 6His 3C-BAP1 C91A
• Parent Plasmid: pET15 6His 3C
• Expressed: 6His-BAP1 C91A
• Genbank: Q92560
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGAATAAGGGCTGGCTGGAGCTGGAGAGCGACCCAGGCCTCTT
CACCCTGCTCGTGGAAGATTTCGGTGTCAAGGGGGTGCAAGTGGAGGAGA
TCTACGACCTTCAGAGCAAATGTCAGGGCCCTGTATATGGATTTATCTTC
CTGTTCAAATGGATCGAAGAGCGCCGGTCCCGGCGAAAGGTCTCTACCTT
GGTGGATGATACGTCCGTGATTGATGATGATATTGTGAATAACATGTTCT
TTGCCCACCAGCTGATACCCAACTCTgcTGCAACTCATGCCTTGCTGAGC
GTGCTCCTGAACTGCAGCAGCGTGGACCTGGGACCCACCCTGAGTCGCAT
GAAGGACTTCACCAAGGGTTTTAGCCCTGAGAGCAAAGGATATGCGATTG
GCAATGCCCCGGAGTTGGCCAAGGCCCATAATAGCCATGCCAGGCCCGAG
CCACGCCACCTCCCTGAGAAGCAGAATGGCCTTAGTGCAGTGCGGACCAT
GGAGGCGTTCCACTTTGTCAGCTATGTGCCTATCACAGGCCGGCTCTTTG
AGCTGGATGGGCTGAAGGTCTACCCCATTGACCATGGGCCCTGGGGGGAG
GACGAGGAGTGGACAGACAAGGCCCGGCGGGTCATCATGGAGCGTATCGG
CCTCGCCACTGCAGGGGAGCCCTACCACGACATCCGCTTCAACCTGATGG
CAGTGGTGCCCGACCGCAGGATCAAGTATGAGGCCAGGCTGCATGTGCTG
AAGGTGAACCGTCAGACAGTACTAGAGGCTCTGCAGCAGCTGATAAGAGT
AACACAGCCAGAGCTGATTCAGACCCACAAGTCTCAAGAGTCACAGCTGC
CTGAGGAGTCCAAGTCAGCCAGCAACAAGTCCCCGCTGGTGCTGGAAGCA
AACAGGGCCCCTGCAGCCTCTGAGGGCAACCACACAGATGGTGCAGAGGA
GGCGGCTGGTTCATGCGCACAAGCCCCATCCCACAGCCCTCCCAACAAAC
CCAAGCTAGTGGTGAAGCCTCCAGGCAGCAGCCTCAATGGGGTTCACCCC
AACCCCACTCCCATTGTCCAGCGGCTGCCGGCCTTTCTAGACAATCACAA
TTATGCCAAGTCCCCCATGCAGGAGGAAGAAGACCTGGCGGCAGGTGTGG
GCCGCAGCCGAGTTCCAGTCCGCCCACCCCAGCAGTACTCAGATGATGAG
GATGACTATGAGGATGACGAGGAGGATGACGTGCAGAACACCAACTCTGC
CCTTAGGTATAAGGGGAAGGGAACAGGGAAGCCAGGGGCATTGAGCGGTT
CTGCTGATGGGCAACTGTCAGTGCTGCAGCCCAACACCATCAACGTCTTG
GCTGAGAAGCTCAAAGAGTCCCAGAAGGACCTCTCAATTCCTCTGTCCAT
CAAGACTAGCAGCGGGGCTGGGAGTCCGGCTGTGGCAGTGCCCACACACT
CGCAGCCCTCACCCACCCCCAGCAATGAGAGTACAGACACGGCCTCTGAG
ATCGGCAGTGCTTTCAACTCGCCACTGCGCTCGCCTATCCGCTCAGCCAA
CCCGACGCGGCCCTCCAGCCCTGTCACCTCCCACATCTCCAAGGTGCTTT
TTGGAGAGGATGACAGCCTGCTGCGTGTTGACTGCATACGCTACAACCGT
GCTGTCCGTGATCTGGGTCCTGTCATCAGCACAGGCCTGCTGCACCTGGC
TGAGGATGGGGTGCTGAGTCCCCTGGCGCTGACAGAGGGTGGGAAGGGTT
CCTCGCCCTCCATCAGACCAATCCAAGGCAGCCAGGGGTCCAGCAGCCCA
GTGGAGAAGGAGGTCGTGGAAGCCACGGACAGCAGAGAGAAGACGGGGAT
GGTGAGGCCTGGCGAGCCCTTGAGTGGGGAGAAATACTCACCCAAGGAGC
TGCTGGCACTGCTGAAGTGTGTGGAGGCTGAGATTGCAAACTATGAGGCG
TGCCTCAAGGAGGAGGTAGAGAAGAGGAAGAAGTTCAAGATTGATGACCA
GAGAAGGACCCACAACTACGATGAGTTCATCTGCACCTTTATCTCCATGC
TGGCTCAGGAAGGCATGCTGGCCAACCTAGTGGAGCAGAACATCTCCGTG
CGGCGGCGCCAAGGGGTCAGCATCGGCCGGCTCCACAAGCAGCGGAAGCC
TGACCGGCGGAAACGCTCTCGCCCCTACAAGGCCAAGCGCCAGTGAGCGG
CCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMNKGWLELESDPGLFTLLVEDFGVKGV
QVEEIYDLQSKCQGPVYGFIFLFKWIEERRSRRKVSTLVDDTSVIDDDIV
NNMFFAHQLIPNSAATHALLSVLLNCSSVDLGPTLSRMKDFTKGFSPESK
GYAIGNAPELAKAHNSHARPEPRHLPEKQNGLSAVRTMEAFHFVSYVPIT
GRLFELDGLKVYPIDHGPWGEDEEWTDKARRVIMERIGLATAGEPYHDIR
FNLMAVVPDRRIKYEARLHVLKVNRQTVLEALQQLIRVTQPELIQTHKSQ
ESQLPEESKSASNKSPLVLEANRAPAASEGNHTDGAEEAAGSCAQAPSHS
PPNKPKLVVKPPGSSLNGVHPNPTPIVQRLPAFLDNHNYAKSPMQEEEDL
AAGVGRSRVPVRPPQQYSDDEDDYEDDEEDDVQNTNSALRYKGKGTGKPG
ALSGSADGQLSVLQPNTINVLAEKLKESQKDLSIPLSIKTSSGAGSPAVA
VPTHSQPSPTPSNESTDTASEIGSAFNSPLRSPIRSANPTRPSSPVTSHI
SKVLFGEDDSLLRVDCIRYNRAVRDLGPVISTGLLHLAEDGVLSPLALTE
GGKGSSPSIRPIQGSQGSSSPVEKEVVEATDSREKTGMVRPGEPLSGEKY
SPKELLALLKCVEAEIANYEACLKEEVEKRKKFKIDDQRRTHNYDEFICT
FISMLAQEGMLANLVEQNISVRRRQGVSIGRLHKQRKPDRRKRSRPYKAK
RQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29318
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee