HERC3

• Plasmid: pGEX6P HALO Thr-HERC3 isoform 1 Lys360-Ala 1050
• Parent Plasmid: pGEX 3C HALO Thr
• Expressed: GST-HALO-HERC3 isoform 1 Lys360-Ala 1050
• Genbank: NP_055421.1 (uni: Q15034-1)
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

ggatccAAATATCATATCGTTAAGCAGATCTTCTCTGGAGGAGACCAGAC
TTTTGTACTTTGCTCCAAATACGAGAATTATTCTCCTGCTGTTGACTTCA
GGACTATGAACCAAGCACATTATACCAGTTTAATAAATGATGAAACCATA
GCAGTTTGGAGACAAAAACTCTCAGAACACAACAATGCAAATACAATCAA
TGGTGTTGTTCAGATATTATCTTCTGCAGCCTGTTGGAATGGAAGTTTTC
TTGAAAAAAAAATTGATGAACATTTTAAAACGAGTCCCAAAATCCCTGGG
ATTGACCTGAACTCAACTAGGGTGTTATTTGAGAAGTTAATGAACTCTCA
GCACTCCATGATTCTAGAACAGATTTTGAACAGTTTTGAAAGTTGTCTGA
TTCCCCAGTTGTCAAGCTCACCACCAGATGTTGAAGCCATGAGAATCTAT
TTAATACTACCTGAGTTTCCCCTACTCCAGGATTCCAAGTATTATATAAC
ATTGACTATTCCCTTGGCTATGGCCATTCTTCGGCTGGATACAAACCCCA
GCAAAGTACTAGATAACTGGTGGTCTCAGGTATGCCCGAAATATTTCATG
AAGCTGGTAAACCTCTATAAAGGTGCAGTCCTTTATCTACTGAGGGGAAG
AAAGACATTCTTAATTCCCGTACTGTTTAACAATTATATCACAGCAGCTC
TCAAACTCTTGGAGAAGTTATATAAGGTAAATCTTAAAGTGAAGCATGTG
GAATATGATACATTTTACATTCCTGAGATTTCCAATCTCGTGGACATTCA
GGAAGACTACCTCATGTGGTTCTTGCATCAAGCAGGGATGAAGGCTAGAC
CATCAATAATACAGGATACTGTAACACTTTGTTCCTACCCTTTCATCTTT
GATGCCCAAGCCAAGACCAAAATGTTACAGACAGATGCTGAACTACAGAT
GCAGGTGGCAGTCAATGGAGCCAACCTGCAGAATGTCTTCATGCTTCTCA
CCCTGGAGCCTCTGCTGGCCAGAAGCCCCTTCCTGGTCCTTCACGTTCGC
AGGAACAACCTTGTTGGAGATGCCCTAAGAGAGCTGAGCATTCATTCTGA
TATTGATTTGAAAAAGCCTCTCAAAGTAATCTTTGATGGTGAAGAAGCAG
TGGATGCCGGTGGTGTTACAAAGGAATTTTTTCTTTTGCTGTTAAAAGAA
CTTTTGAATCCCATCTATGGAATGTTTACCTACTATCAAGATTCAAATCT
CTTGTGGTTTTCAGACACGTGTTTTGTAGAGCACAACTGGTTTCACTTGA
TTGGTATAACCTGTGGACTAGCTATCTACAACTCCACTGTGGTCGATCTC
CACTTCCCATTGGCTCTCTACAAGAAGTTACTCAATGTAAAGCCTGGCTT
GGAAGACTTAAAGGAGTTGTCACCCACTGAAGGAAGGAGTCTCCAAGAGC
TTTTAGATTACCCCGGGGAGGATGTGGAGGAGACTTTCTGCCTCAACTTC
ACGATCTGCCGAGAAAGCTATGGAGTGATTGAACAGAAGAAGCTGATACC
TGGGGGAGATAATGTAACTGTGTGCAAGGATAACAGGCAGGAATTTGTGG
ATGCTTATGTGAATTATGTCTTCCAAATCTCAGTTCATGAATGGTACACA
GCCTTCTCTAGTGGCTTCCTAAAGGTGTGTGGTGGCAAAGTACTTGAGCT
CTTCCAGCCTTCAGAACTGAGGGCTATGATGGTGGGGAACAGCAACTACA
ACTGGGAAGAACTGGAAGAGACTGCCATCTACAAGGGAGATTACTCGGCC
ACACATCCCACTGTAAAACTATTTTGGGAAACATTTCATGAGTTTCCATT
GGAAAAGAAGAAGAAGTTTCTCTTGTTCCTGACAGGCAGCGATCGGATTC
CCATCTACGGCATGGCCAGTCTGCAGATTGTCATCCAGTCCACAGCCAGC
GGGGAGGAGTACTTGCCGGTGGCCCACACTTGCTACAACCTTCTTGACCT
CCCCAAGTACAGCAGCAAAGAGATTCTGAGTGCCCGGCTGACCCAGGCCC
TTGACAACTATGAAGGGTTTAGTTTGGCCTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSKYHIVKQIFSGGDQ
TFVLCSKYENYSPAVDFRTMNQAHYTSLINDETIAVWRQKLSEHNNANTI
NGVVQILSSAACWNGSFLEKKIDEHFKTSPKIPGIDLNSTRVLFEKLMNS
QHSMILEQILNSFESCLIPQLSSSPPDVEAMRIYLILPEFPLLQDSKYYI
TLTIPLAMAILRLDTNPSKVLDNWWSQVCPKYFMKLVNLYKGAVLYLLRG
RKTFLIPVLFNNYITAALKLLEKLYKVNLKVKHVEYDTFYIPEISNLVDI
QEDYLMWFLHQAGMKARPSIIQDTVTLCSYPFIFDAQAKTKMLQTDAELQ
MQVAVNGANLQNVFMLLTLEPLLARSPFLVLHVRRNNLVGDALRELSIHS
DIDLKKPLKVIFDGEEAVDAGGVTKEFFLLLLKELLNPIYGMFTYYQDSN
LLWFSDTCFVEHNWFHLIGITCGLAIYNSTVVDLHFPLALYKKLLNVKPG
LEDLKELSPTEGRSLQELLDYPGEDVEETFCLNFTICRESYGVIEQKKLI
PGGDNVTVCKDNRQEFVDAYVNYVFQISVHEWYTAFSSGFLKVCGGKVLE
LFQPSELRAMMVGNSNYNWEELEETAIYKGDYSATHPTVKLFWETFHEFP
LEKKKKFLLFLTGSDRIPIYGMASLQIVIQSTASGEEYLPVAHTCYNLLD
LPKYSSKEILSARLTQALDNYEGFSLA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28596
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee