Ubiquitin

• Plasmid: pTXB1 Ubiquitin 1-76 S65A-Ubiquitin 1-75 (both copies codon opitmised)-Mxe intein/chitin binding domain
• Parent Plasmid: pTXB1
• Expressed: Ubiquitin 1-76 S65A-Ubiquitin 1-75 (both copies codon opitmised)-Mxe intein/chitin binding domain
• Genbank: codon optimised
• Species: Human

Sequence of Insert

CATATGCAGATCTTCGTCAAGACGTTAACCGGTAAAACCATAACTCTAGAAGTGGAACCGAGCGATACCATCGAAAACGTGAAAGCGAAAATCCAGGATA
AAGAAGGCATCCCGCCGGATCAGCAGCGTCTGATCTTTGCGGGCAAACAGCTGGAAGATGGCCGCACCCTGTCTGATTATAACATCCAGAAAGAGGCCAC
CCTGCATCTGGTCTTAAGACTGCGTGGCGGTATGCAGATCTTCGTCAAGACGTTAACCGGTAAAACCATAACTCTAGAAGTGGAACCGAGCGATACCATC
GAAAACGTGAAAGCGAAAATCCAGGATAAAGAAGGCATCCCGCCGGATCAGCAGCGTCTGATCTTTGCGGGCAAACAGCTGGAAGATGGCCGCACCCTGT
CTGATTATAACATCCAGAAAGAGTCGACCCTGCATCTGGTCTTAAGACTGCGTGGCTGCATCACGGGAGATGCACTAGT

Amino Acid Sequence

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKEATLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE
NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGCITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPVLADR
LFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAVIQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDA
QAIADELTDGRFYYAKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHATGLTGLNSGLTTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWE
PSNVPALWQLQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU53452
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee