JIP4_Human isoform 1

• Plasmid: pET15 6HIS SUMO-JIP4
• Parent Plasmid: pET15 6HIS SUMO
• Expressed: 6HIS SUMO-JIP4
• Genbank: NM_001130528.2
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

ggatccATGGAGCTGGAGGACGGTGTGGTGTATCAGGAGGAGCCCGGCGG
CTCCGGGGCCGTGATGTCGGAGCGGGTGTCCGGCCTGGCCGGCTCCATCT
ACCGCGAGTTCGAGCGGCTTATCGGGCGCTATGACGAGGAGGTGGTCAAA
GAGCTGATGCCGCTGGTGGTGGCTGTGCTGGAGAACCTGGACTCGGTGTT
CGCGCAGGACCAGGAGCACCAGGTGGAGCTGGAGCTGCTGCGGGACGACA
ACGAGCAGCTCATCACCCAGTACGAGCGGGAGAAGGCGCTGCGCAAGCAC
GCTGAGGAGAAATTCATTGAATTTGAAGACTCTCAAGAACAGGAAAAAAA
GGACTTACAGACCCGAGTGGAATCTTTAGAATCTCAAACAAGACAACTTG
AGCTGAAAGCGAAAAACTATGCTGACCAGATTAGCAGACTTGAAGAAAGA
GAAGCAGAACTGAAGAAGGAATATAATGCATTACATCAAAGACACACTGA
GATGATCCATAATTATATGGAACATTTAGAAAGAACAAAACTTCATCAGC
TCTCAGGGAGTGATCAACTAGAATCCACAGCTCATAGTAGAATTAGAAAA
GAACGCCCTATATCATTAGGAATTTTCCCATTACCTGCTGGAGATGGATT
GCTTACACCTGATGCTCAGAAAGGAGGAGAGACCCCTGGATCTGAGCAAT
GGAAATTTCAGGAATTAAGTCAACCACGTTCTCATACCAGCCTGAAGGTC
AGCAATAGTCCTGAACCTCAGAAGGCTGTAGAACAGGAGGATGAGCTTTC
TGATGTTAGCCAAGGCGGATCTAAAGCTACCACTCCAGCATCAACAGCTA
ATTCAGATGTGGCAACAATTCCTACTGATACTCCCTTAAAGGAAGAAAAC
GAAGGATTTGTGAAGGTTACAGATGCGCCAAATAAATCAGAGATAAGCAA
ACACATTGAAGTACAGGTAGCCCAGGAAACTAGAAATGTATCTACTGGCT
CTGCTGAAAATGAAGAAAAGTCAGAAGTTCAAGCAATCATCGAATCTACT
CCTGAGCTGGATATGGACAAAGATCTCAGTGGATATAAAGGTTCAAGCAC
TCCCACCAAAGGCATAGAGAACAAAGCTTTTGATCGCAATACAGAATCTC
TCTTTGAAGAACTGTCTTCAGCTGGCTCAGGCCTAATAGGAGATGTGGAT
GAAGGAGCAGATTTACTAGGAATGGGTCGGGAAGTTGAGAATCTTATATT
AGAAAATACACAACTGTTGGAAACCAAAAATGCTTTGAACATAGTGAAGA
ATGATTTGATAGCAAAAGTGGATGAACTGACCTGTGAGAAAGATGTGCTG
CAAGGGGAATTGGAGGCTGTGAAGCAAGCCAAACTGAAACTAGAGGAAAA
GAACAGAGAATTGGAGGAAGAGCTTAGGAAAGCTCGGGCAGAAGCTGAAG
ATGCAAGGCAAAAAGCAAAAGATGACGATGATAGTGATATTCCCACAGCC
CAGAGGAAACGGTTTACTAGAGTAGAAATGGCCCGTGTTCTCATGGAGCG
AAACCAGTATAAAGAGAGATTGATGGAGCTTCAGGAAGCTGTTCGATGGA
CAGAGATGATTCGGGCATCACGAGAAAATCCAGCCATGCAGGAAAAAAAA
AGGTCAAGCATTTGGCAGTTTTTCAGCCGACTTTTCAGCTCCTCAAGTAA
CACGACTAAGAAGCCTGAACCACCTGTTAATCTGAAGTACAATGCACCCA
CGTCTCATGTTACTCCGTCCGTCAAGAAAAGAAGCAGCACCTTATCTCAG
CTCCCTGGGGATAAGTCCAAAGCCTTTGATTTCCTTAGTGAAGAAACTGA
AGCTAGTTTAGCCTCACGCAGAGAACAAAAGAGAGAGCAGTATCGTCAGG
TAAAAGCACATGTTCAGAAGGAAGACGGTAGAGTGCAGGCTTTTGGCTGG
AGTCTGCCTCAGAAGTACAAACAGGTAACCAATGGTCAAGGTGAAAATAA
GATGAAAAATTTACCTGTGCCTGTCTATCTCAGACCTCTGGATGAAAAAG
ATACATCAATGAAGCTGTGGTGTGCTGTTGGAGTCAATTTATCTGGTGGG
AAGACCAGAGATGGTGGTTCTGTTGTTGGAGCAAGTGTATTTTACAAGGA
TGTTGCTGGTTTGGATACAGAAGGCAGTAAACAGCGAAGTGCCTCTCAGA
GTAGTTTAGATAAGTTAGATCAGGAACTTAAGGAACAGCAGAAGGAGTTA
AAAAATCAAGAAGAATTATCCAGTCTAGTTTGGATCTGTACCAGCACTCA
TTCGGCTACAAAAGTTCTTATTATTGATGCTGTTCAACCTGGCAACATCC
TAGACAGTTTCACTGTTTGCAACTCTCATGTTCTGTGCATTGCAAGTGTG
CCAGGTGCACGAGAAACAGACTACCCTGCAGGAGAAGATCTTTCAGAATC
TGGTCAGGTAGACAAAGCATCTTTATGTGGAAGTATGACAAGCAACAGCT
CAGCAGAGACAGACAGCCTGTTAGGAGGCATCACAGTGGTTGGTTGTTCT
GCAGAAGGTGTGACGGGAGCTGCCACTTCCCCTAGTACAAATGGTGCTTC
TCCAGTGATGGATAAACCACCAGAAATGGAAGCAGAAAATAGTGAGGTTG
ATGAAAATGTTCCAACAGCAGAAGAAGCAACTGAAGCTACAGAAGGGAAT
GCGGGGTCAGCTGAAGACACAGTGGACATCTCCCAAACTGGCGTCTACAC
AGAGCATGTCTTTACAGATCCTTTGGGAGTTCAGATCCCAGAAGACCTCT
CCCCAGTGTATCAGTCGAGCAATGACTCAGATGCATATAAAGATCAAATA
TCAGTACTGCCAAATGAACAAGACTTGGTGAGAGAAGAAGCCCAGAAAAT
GAGTAGTCTTTTACCAACTATGTGGCTTGGAGCTCAAAATGGCTGTTTGT
ATGTCCATTCATCTGTAGCCCAGTGGAGGAAATGTCTCCATTCCATTAAA
CTTAAAGATTCGATTCTCAGTATTGTACACGTGAAGGGAATCGTGTTAGT
AGCCCTGGCTGACGGCACCCTTGCAATCTTTCACAGAGGAGTGGATGGGC
AGTGGGATTTGTCAAACTATCACCTCTTAGACCTTGGACGGCCTCATCAT
TCCATCCGTTGCATGACTGTGGTACATGACAAAGTCTGGTGTGGCTATAG
GAACAAAATCTATGTGGTGCAGCCAAAGGCCATGAAAATAGAGAAATCTT
TTGATGCACATCCCAGGAAGGAGAGCCAAGTGCGACAGCTTGCGTGGGTG
GGGGATGGCGTGTGGGTCTCCATTCGCTTGGATTCTACGCTCCGTCTCTA
TCATGCACACACTTATCAACATCTACAGGATGTGGACATTGAGCCTTATG
TAAGCAAAATGTTAGGTACTGGAAAACTGGGCTTCTCTTTTGTGAGAATT
ACAGCTCTTATGGTGTCTTGTAATCGTTTGTGGGTGGGGACAGGAAATGG
TGTCATTATCTCCATCCCATTGACAGAAACAAATAAAACCTCAGGTGTAC
CAGGAAATCGTCCTGGAAGTGTAATCCGTGTATATGGTGATGAAAACAGT
GATAAAGTGACTCCAGGGACATTTATACCCTATTGTTCAATGGCACATGC
ACAGCTTTGCTTCCATGGGCACCGGGATGCTGTGAAATTCTTTGTGGCAG
TCCCAGGTCAAGTCATCAGCCCACAAAGTAGCAGTAGTGGCACGGATCTG
ACGGGTGACAAAGCAGGGCCATCTGCACAGGAGCCTGGTAGTCAGACGCC
CTTGAAGTCTATGCTTGTCATCAGTGGAGGAGAGGGCTACATCGACTTCC
GAATGGGTGATGAAGGTGGAGAATCAGAACTTCTTGGAGAGGATCTTCCA
CTTGAACCTTCTGTCACCAAAGCAGAAAGGAGTCACTTGATAGTGTGGCA
AGTGATGTATGGCAATGAGTGAgcggccgc

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMELEDGVVYQEEPGGSGAVMSERVSGLAGSIYREFERLIGRYDEEV
VKELMPLVVAVLENLDSVFAQDQEHQVELELLRDDNEQLITQYEREKALR
KHAEEKFIEFEDSQEQEKKDLQTRVESLESQTRQLELKAKNYADQISRLE
EREAELKKEYNALHQRHTEMIHNYMEHLERTKLHQLSGSDQLESTAHSRI
RKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSL
KVSNSPEPQKAVEQEDELSDVSQGGSKATTPASTANSDVATIPTDTPLKE
ENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIE
STPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGD
VDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKD
VLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIP
TAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQE
KKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTL
SQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAF
GWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLS
GGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQK
ELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIA
SVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVG
CSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATE
GNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKD
QISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHS
IKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRP
HHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLA
WVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFV
RITALMVSCNRLWVGTGNGVIISIPLTETNKTSGVPGNRPGSVIRVYGDE
NSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGT
DLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGED
LPLEPSVTKAERSHLIVWQVMYGNE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28521
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee