RNF214

• Plasmid: pET15 6His MBP TEV RNF214
• Parent Plasmid: pET15 6HIS MBP DU
• Expressed: 6His MBP TEV RNF214
• Genbank: NM_001077239.1 GI:116235455
• Tag: 6His
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCATGGCAGCGTCTGAGGTTGCTGGTGTTGTGGCCAATGCCCCCAGTCCTCCGGAATCTTCTAGTTTATGTGCTTCCAAATCAGACGAAGGTCTCCCAGATGGTCTAAGCACCAAAGACTCTGCACAGAAGCAGAAGAACTCGCCTCTGTTGAGTGTAAGTAGCCAAACAATAACCAAGGAGAATAACAGAAATGTCCATTTGGAGCACTCAGAGCAGAATCCTGGTTCATCAGCAGGTGACACCTCAGCAGCGCACCAGGTGGTTTTAGGAGAAAACTTGATAGCCACAGCCCTTTGTCTTTCTGGCAGTGGGTCTCAGTCTGATTTGAAGGATGTGGCCAGCACAGCAGGAGAGGAGGGGGACACAAGCCTTCGGGAGAGCCTCCATCCAGTCACTCGGTCTCTTAAGGCAGGGTGCCATACTAAGCAGCTTGCCTCCAGGAATTGCTCTGAAGAGAAATCCCCACAAACCTCCATCCTAAAGGAAGGTAACAGGGACACAAGCTTGGATTTCCGACCTGTAGTGTCTCCAGCAAATGGGGTTGAAGGAGTCCGAGTGGATCAGGATGATGATCAAGATAGCTCTTCCCTGAAGCTTTCTCAGAACATTGCTGTACAGACTGACTTTAAGACAGCTGATTCAGAGGTAAACACAGATCAAGATATTGAAAAGAATTTGGATAAAATGATGACAGAGAGAACCCTGTTGAAAGAGCGTTACCAGGAGGTCCTGGACAAACAGAGGCAAGTGGAGAATCAGCTCCAAGTGCAATTAAAGCAGCTTCAGCAAAGGAGAGAAGAGGAAATGAAGAATCACCAGGAGATATTAAAGGCTATTCAGGATGTGACAATAAAGCGGGAAGAAACAAAGAAGAAGATAGAGAAAGAGAAGAAGGAGTTTTTGCAGAAGGAGCAGGATCTGAAAGCTGAAATTGAGAAGCTTTGTGAGAAGGGCAGAAGAGAGGTGTGGGAAATGGAACTGGATAGACTCAAGAATCAGGATGGCGAAATAAATAGGAACATTATGGAAGAGACTGAACGGGCCTGGAAGGCAGAGATCTTATCACTAGAGAGCCGGAAAGAGTTACTGGTACTGAAACTAGAAGAAGCAGAAAAAGAGGCAGAATTGCACCTTACTTACCTCAAGTCAACTCCCCCAACACTGGAGACAGTTCGTTCCAAACAGGAGTGGGAGACGAGACTGAATGGAGTTCGGATAATGAAAAAGAATGTTCGTGACCAATTTAATAGTCATATCCAGTTAGTGAGGAACGGAGCCAAGCTGAGCAGCCTTCCTCAAATCCCTACTCCCACTTTACCTCCACCCCCATCAGAGACAGACTTCATGCTTCAGGTGTTTCAACCCAGTCCCTCTCTGGCTCCTCGGATGCCCTTCTCCATTGGGCAGGTCACAATGCCCATGGTTATGCCCAGTGCAGATCCCCGCTCCTTGTCTTTCCCAATCCTGAACCCTGCCCTTTCCCAGCCCAGCCAGCCTTCCTCACCCCTTCCTGGCTCCCATGGCAGAAATAGCCCTGGCTTGGGTTCCCTTGTCAGCCCCCACGGTCCACACATGCCCCCTGCCGCCTCCATCCCACCTCCCCCAGGCTTGGGCGGTGTTAAGGCTTCTGCTGAAACTCCCCGGCCCCAACCAGTAGACAAACTGGAGAAGATCCTGGAGAAGCTGCTGACCCGGTTCCCACAGTGCAATAAGGCCCAGATGACCAACATTCTTCAGCAGATCAAGACAGCACGTACCACCATGGCAGGCCTGACCATGGAGGAACTTATCCAGTTGGTTGCTGCACGACTGGCAGAACATGAGCGGGTGGCAGCAAGTACTCAGCCACTTGGTCGCATCCGGGCCTTGTTCCCTGCTCCACTGGCCCAAATCAGTACCCCAATGTTCTTGCCTTCTGCCCAAGTTTCATATCCTGGAAGGTCTTCACATGCTCCAGCCACCTGTAAGCTATGTCTAATGTGCCAGAAACTCGTCCAGCCCAGTGAGCTGCATCCAATGGCGTGTACCCATGTATTGCACAAGGAGTGTATCAAATTCTGGGCCCAGACCAACACAAATGACACTTGTCCCTTTTGTCCAACTCTTAAATGA

Amino Acid Sequence

MGSSHHHHHHGSSMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQ
DKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNA
GAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLT
DEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGENLY
FQGHMGSMAASEVAGVVANAPSPPESSSLCASKSDEGLPDGLSTKDSAQKQKNSPLLSVSSQTITKENNRNVHLEHSEQNPGSSAGDTSAAHQVVLGENL
IATALCLSGSGSQSDLKDVASTAGEEGDTSLRESLHPVTRSLKAGCHTKQLASRNCSEEKSPQTSILKEGNRDTSLDFRPVVSPANGVEGVRVDQDDDQD
SSSLKLSQNIAVQTDFKTADSEVNTDQDIEKNLDKMMTERTLLKERYQEVLDKQRQVENQLQVQLKQLQQRREEEMKNHQEILKAIQDVTIKREETKKKI
EKEKKEFLQKEQDLKAEIEKLCEKGRREVWEMELDRLKNQDGEINRNIMEETERAWKAEILSLESRKELLVLKLEEAEKEAELHLTYLKSTPPTLETVRS
KQEWETRLNGVRIMKKNVRDQFNSHIQLVRNGAKLSSLPQIPTPTLPPPPSETDFMLQVFQPSPSLAPRMPFSIGQVTMPMVMPSADPRSLSFPILNPAL
SQPSQPSSPLPGSHGRNSPGLGSLVSPHGPHMPPAASIPPPPGLGGVKASAETPRPQPVDKLEKILEKLLTRFPQCNKAQMTNILQQIKTARTTMAGLTM
EELIQLVAARLAEHERVAASTQPLGRIRALFPAPLAQISTPMFLPSAQVSYPGRSSHAPATCKLCLMCQKLVQPSELHPMACTHVLHKECIKFWAQTNTN
DTCPFCPTLK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU53099
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee