mouse FancI/human FANCL

• Synonyms: FAAP43; PHF9; POG
• Plasmid: pET15 6His TEV codon optimised mouse FancI M1-E1301 13TGS FANCL Q109-E294
• Parent Plasmid: pET15 6His 3C
• Expressed: 6His TEV codon optimised mouse FancI M1-E1301 13TGS FANCL Q109-E294
• Genbank: mouse FancI:codon optimised NM_145946.2 Human FANCL: NM_001114636.1 GI:167860137
• Tag: 6His

Sequence of Insert

CCATGGGCAGCAGCCATCACCATCACCATCACGAAAACCTGTATTTTCAGATGGACCTGAAGATCCTGTCCCTGGCTACCGACAAGACCACCGACAAGCTGCAAGAGTTCCTGCAGACCCTGAAGGACGACGACCTGGCTTCCCTGCTGCAGAACCAGGCTGTGAAGGGTCGTGCTGTGGGCACCCTGCTGCGTGCTGTGCTGAAGGGTTCCCCCTGCTCCGAAGAGGACGGTGCTCTGCGCCGTTACAAGATCTACTCCTGCTGCATCCAGCTGGTCGAGTCCGGCGACCTCCAGCAGGATGTGGCTTCCGAGATCATCGGCCTGCTGATGCTCGAGGTGCACCACTTCCCTGGTCCTCTGCTGGTGGACCTGGCCTCCGATTTCGTGGGTGCTGTGCGCGAGGACCGTCTGGTCAACGGCAAGTCCCTGGAACTGCTGCCCATCATCCTGACCGCTCTGGCTACCAAGAAGGAAGTCCTGGCTTGCGGCAAGGGCGACCTGAACGGCGAAGAGTACAAGCGTCAGCTGATCGACACCCTGTGCTCCGTGCGTTGGCCCCAGCGTTACATGATCCAGCTGACCTCCGTGTTCAAGGACGTGTGCCTGACCCCCGAAGAGATGAACCTGGTGGTGGCTAAGGTGCTGACCATGTTCTCCAAGCTGAACCTGCAAGAGATCCCCCCACTGGTGTACCAGCTGCTGGTCCTGTCCTCCAAGGGCTCCCGTCGTTCTGTGCTGGACGGTATCATCGCTTTCTTCCGTGAACTGGACAAGCAGCACCGCGAGGAACAGTCCTCCGACGAGCTGTCCGAGCTGATCACCGCTCCTGCTGACGAACTGTACCACGTCGAGGGCACCGTGATCCTGCACATCGTGTTCGCTATCAAGCTGGACTGCGAGCTGGGTCGCGAGCTGCTGAAGCACCTGAAGGCTGGTCAACAGGGCGACCCCTCCAAGTGCCTGTGCCCCTTCTCTATCGCTCTGCTGCTGTCCCTGACCCGTATCCAGCGTTTCGAAGAACAGGTGTTCGACCTGCTCAAGACCTCCGTCGTGAAGTCCTTCAAGGACCTGCAGCTCCTGCAGGGTTCCAAGTTCCTCCAGACTCTGGTGCCCCAGAGGACCTGCGTGTCCACCATGATCCTGGAAGTCGTGCGCAACTCCGTGCACTCCTGGGACCACGTGACCCAGGGCCTGATCGAGTTCGGTTTCATCCTGATGGACTCCTACGGTCCCAAGAAGATCCTCGACGGCAAGGCTGTCGAGATCGGCACCTCCCTGTCCAAGATGACCAACCAGCACGCCTGCAAGCTGGGTGCTAACATCCTCCTGGAAACCTTCAAGATCCACGAGATGATCCGCCAAGAAATCCTGGAACAGGTGCTGAACCGTGTCGTGACCCGTACCTCCTCCCCTATCAACCACTTCCTGGACCTGTTCTCCGACATCATTATGTACGCTCCTCTGATCCTCCAGAACTGCTCCAAAGTGACCGAGACTTTCGACTACCTGACCTTCCTGCCTCTGCAGACCGTGCAGGGACTGCTGAAGGCCGTGCAGCCCCTGCTGAAGATCTCCATGTCCATGCGCGACTCCCTGATCCTGGTGCTGCGCAAGGCTATGTTCGCTTCCCAGCTGGACGCTCGCAAGTCCGCTGTGGCTGGTTTCCTGCTGCTCCTGAAGAACTTCAAGGTGCTGGGTTCCCTGCCATCCTCCCAGTGCACCCAGTCCATCGGTGTCACCCAAGTGCGCGTGGACGTGCACTCTCGTTACTCCGCTGTCGCTAACGAAACCTTCTGCCTGGAAATCATCGACTCCCTCAAGCGTTCCCTGGGCCAGCAGGCTGACATCCGTCTGATGCTGTACGACGGTTTCTACGACGTGCTGCGTCGTAACTCTCAGCTGGCTTCCTCCATCATGCAGACTCTGTTCTCCCAGCTCAAGCAGTTCTACGAGCCCGAGCCCGACCTGCTGCCTCCTCTGAAGTTGGGAGCTTGCGTGCTGACTCAGGGTTCCCAGATCTTCCTGCAAGAACCCCTGGACCACCTCCTGTCCTGCATCCAACACTGCCTGGCTTGGTACAAGTCCCGTGTGGTGCCCCTGCAGCAAGGCGACGAGGGCGAGGAAGAGGAAGAAGAACTGTACTCCGAGCTGGACGACATGCTGGAATCCATCACCGTGCGTATGATCAAGTCCGAGTTGGAGGACTTCGAGTTGGACAAGTCCGCCGACTTCTCCCAGAACACCAACGTGGGCATCAAGAACAACATCTGCGCTTGCCTGATCATGGGCGTGTGCGAGGTGCTGATGGAGTACAACTTCTCCATCTCGAACTTCAGCAAGTCCAAGTTCGAAGAGATCCTGAGCCTGTTCACTTGCTACAAGAAGTTCAGCGACATCCTGTCCGAGAAGGCTGGCAAGGGCAAGGCTAAGATGACCTCCAAGGTGTCCGACTCTCTGCTGTCTCTCAAGTTCGTGTCTGACCTGCTGACCGCCCTGTTCCGTGACTCCATCCAGTCCCACGAGGAATCCCTGTCCGTGCTGAGATCCTCCGGCGAGTTCATGCACTACGCTGTGAACGTGACCCTCCAGAAGATCCAGCAGCTCATCCGTACCGGTCACGTGTCCGGACCTGACGGCCAGAACCCCGACAAGATCTTCCAGAACCTGTGCGACATCACCCGTGTGCTGCTGTGGCGTTACACCTCCATCCCCACCTCCGTGGAAGAGTCCGGAAAGAAGGAAAAGGGCAAGTCCATCTCCCTGCTGTGCCTGGAAGGCCTGCAAAAGACCTTCTCCGTGGTGCTCCAGTTCTACCAACCCAAGGTGCAGCAATTCCTGCAGGCTCTGGACGTGATGGGTACTGAAGAGGAAGAGGCTGGCGTCACCGTGACCCAGAGGGCTAGCTTCCAGATCCGTCAGTTCCAGCGTTCTCTGCTCAACCTGCTGTCCAGCGAAGAGGATGACTTCAACTCCAAGGAAGCCCTGCTGCTGATCGCCGTGCTGTCCACCCTGTCCCGTCTGCTGGAACCTACCTCCCCACAGTTCGTGCAGATGCTGTCCTGGACTTCCAAGATCTGCAAGGAATACTCTCAAGAGGACGCCAGCTTCTGCAAGTCTCTGATGAACCTCTTCTTCTCCCTCCACGTGCTGTACAAGTCTCCCGTGACCCTGCTGAGGGACCTGTCCCAGGACATCCACGGTCAACTGGGAGACATCGACCAGGACGTGGAAATCGAAAAGACCGACCACTTCGCTGTGGTCAACCTGCGTACCGCTGCTCCCACTGTGTGCCTGCTGGTGTTGTCCCAGGCCGAGAAGGTCCTGGAAGAGGTCGACTGGCTGATCGCTAAGATCAAGGGTTCCGCTAACCAGGAAACCCTGTCTGACAAAGTGACTCCCGAGGACGCCTCCTCCCAGGCTGTTCCTCCTACCCTGCTCATCGAGAAGGCCATCGTGATGCAGCTGGGTACTCTGGTCACCTTCTTCCACGAACTGGTGCAGACTGCTCTGCCCTCCGGTTCCTGCGTGGACACCCTCCTGAAGGGCCTGTCTAAGATCTACAGCACCCTGACCGCCTTCGTGAAGTACTACCTGCAAGTGTGCCAGTCCTCCCGTGGTATCCCCAACACCGTCGAGAAGCTGGTCAAGCTGTCCGGTTCCCACCTGACCCCTGTGTGCTACTCCTTCATCTCCTACGTGCAGAACAAGTCCTCTGACGCTCCCAAGTGCAGCGAAAAGGAAAAGGCTGCTGTCTCCACCACAATGGCTAAGGTCCTGCGCGAGACTAAGCCCATCCCCAACCTGGTGTTCGCCATCGAGCAGTACGAGAAGTTCCTGATCCAGTTGTCCAAGAAGTCCAAAGTCAACCTGATGCAGCACATGAAGCTGTCCACTTCCCGCGATTTCAAGATCAAGGGCAGCGTGCTGGACATGGTGCTCCGCGAGGATGAGACCGGATCCACCGGGAGCACAGGCTCTACGGGATCGACTGGATCGACCGGTTCAACGGGGAGCACTGGTTCAACAGGTTCCACCGGAAGCACAGGAAGTACAGGGTCCACTGGAAGTATGGGACAGTTCTACTCAAGCCTTATTGAAGAGATAGGAACTCTTGGTTGGGATAAACTTGTGTATGCGGATACCTGCTTCAGTACCATCAAGTTAAAAGCAGAAGATGCTTCTGGTAGAGAGCATTTAATCACTCTCAAGTTGAAGGCAAAGTATCCTGCAGAATCACCAGATTATTTTGTGGATTTTCCTGTTCCATTTTGTGCCTCCTGGACACCTCAGGTAAATTCTCCTCAGAGCTCCTTAATAAGCATTTATAGTCAGTTTTTGGCAGCAATAGAATCACTAAAGGCATTCTGGGATGTTATGGATGAAATCGATGAGAAGACCTGGGTACTTGAGCCAGAAAAACCTCCACGGAGTGCAACAGCACGCAGAATTGCATTAGGTAATAATGTTTCCATAAATATAGAGGTAGACCCCAGGCATCCTACTATGCTTCCTGAGTGCTTCTTTCTTGGAGCTGACCATGTGGTAAAACCCCTGGGAATTAAGCTGAGCAGGAACATACATTTGTGGGACCCAGAAAATAGTGTGTTACAAAATTTGAAAGATGTTTTAGAATGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHENLYFQMDLKILSLATDKTTDKLQEFLQTLKDDDLASLLQNQAVKGRAVGTLLRAVLKGSPCSEEDGALRRYKIYSCCIQLVESGDLQQD
VASEIIGLLMLEVHHFPGPLLVDLASDFVGAVREDRLVNGKSLELLPIILTALATKKEVLACGKGDLNGEEYKRQLIDTLCSVRWPQRYMIQLTSVFKDV
CLTPEEMNLVVAKVLTMFSKLNLQEIPPLVYQLLVLSSKGSRRSVLDGIIAFFRELDKQHREEQSSDELSELITAPADELYHVEGTVILHIVFAIKLDCE
LGRELLKHLKAGQQGDPSKCLCPFSIALLLSLTRIQRFEEQVFDLLKTSVVKSFKDLQLLQGSKFLQTLVPQRTCVSTMILEVVRNSVHSWDHVTQGLIE
FGFILMDSYGPKKILDGKAVEIGTSLSKMTNQHACKLGANILLETFKIHEMIRQEILEQVLNRVVTRTSSPINHFLDLFSDIIMYAPLILQNCSKVTETF
DYLTFLPLQTVQGLLKAVQPLLKISMSMRDSLILVLRKAMFASQLDARKSAVAGFLLLLKNFKVLGSLPSSQCTQSIGVTQVRVDVHSRYSAVANETFCL
EIIDSLKRSLGQQADIRLMLYDGFYDVLRRNSQLASSIMQTLFSQLKQFYEPEPDLLPPLKLGACVLTQGSQIFLQEPLDHLLSCIQHCLAWYKSRVVPL
QQGDEGEEEEEELYSELDDMLESITVRMIKSELEDFELDKSADFSQNTNVGIKNNICACLIMGVCEVLMEYNFSISNFSKSKFEEILSLFTCYKKFSDIL
SEKAGKGKAKMTSKVSDSLLSLKFVSDLLTALFRDSIQSHEESLSVLRSSGEFMHYAVNVTLQKIQQLIRTGHVSGPDGQNPDKIFQNLCDITRVLLWRY
TSIPTSVEESGKKEKGKSISLLCLEGLQKTFSVVLQFYQPKVQQFLQALDVMGTEEEEAGVTVTQRASFQIRQFQRSLLNLLSSEEDDFNSKEALLLIAV
LSTLSRLLEPTSPQFVQMLSWTSKICKEYSQEDASFCKSLMNLFFSLHVLYKSPVTLLRDLSQDIHGQLGDIDQDVEIEKTDHFAVVNLRTAAPTVCLLV
LSQAEKVLEEVDWLIAKIKGSANQETLSDKVTPEDASSQAVPPTLLIEKAIVMQLGTLVTFFHELVQTALPSGSCVDTLLKGLSKIYSTLTAFVKYYLQV
CQSSRGIPNTVEKLVKLSGSHLTPVCYSFISYVQNKSSDAPKCSEKEKAAVSTTMAKVLRETKPIPNLVFAIEQYEKFLIQLSKKSKVNLMQHMKLSTSR
DFKIKGSVLDMVLREDETGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSTGSMGQFYSSLIEEIGTLGWDKLVYADTCFSTIKLKAEDASGREHLI
TLKLKAKYPAESPDYFVDFPVPFCASWTPQVNSPQSSLISIYSQFLAAIESLKAFWDVMDEIDEKTWVLEPEKPPRSATARRIALGNNVSINIEVDPRHP
TMLPECFFLGADHVVKPLGIKLSRNIHLWDPENSVLQNLKDVLE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU50968
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee