FancI (mouse)

• Plasmid: pFastbac1 mouse FancI M1-E1301-6His (codon-optimised) (killed internal NcoI)
• Parent Plasmid: pFastbac1
• Expressed: mouse FancI M1-E1301-TEV-6His
• Genbank: Protein sequence matches NP_666058.2
• Tag: 6His
• Species: Mouse

Sequence of Insert

GCGGCCGCTGCCACCATGGACCTGAAGATCCTGTCCCTGGCTACCGACAAGACCACCGACAAGCTGCAAGAGTTCCTGCAGACCCTGAAGGACGACGACCTGGCTTCCCTGCTGCAGAACCAGGCTGTGAAGGGTCGTGCTGTGGGCACCCTGCTGCGTGCTGTGCTGAAGGGTTCCCCCTGCTCCGAAGAGGACGGTGCTCTGCGCCGTTACAAGATCTACTCCTGCTGCATCCAGCTGGTCGAGTCCGGCGACCTCCAGCAGGATGTGGCTTCCGAGATCATCGGCCTGCTGATGCTCGAGGTGCACCACTTCCCTGGTCCTCTGCTGGTGGACCTGGCCTCCGATTTCGTGGGTGCTGTGCGCGAGGACCGTCTGGTCAACGGCAAGTCCCTGGAACTGCTGCCCATCATCCTGACCGCTCTGGCTACCAAGAAGGAAGTCCTGGCTTGCGGCAAGGGCGACCTGAACGGCGAAGAGTACAAGCGTCAGCTGATCGACACCCTGTGCTCCGTGCGTTGGCCCCAGCGTTACATGATCCAGCTGACCTCCGTGTTCAAGGACGTGTGCCTGACCCCCGAAGAGATGAACCTGGTGGTGGCTAAGGTGCTGACCATGTTCTCCAAGCTGAACCTGCAAGAGATCCCCCCACTGGTGTACCAGCTGCTGGTCCTGTCCTCCAAGGGCTCCCGTCGTTCTGTGCTGGACGGTATCATCGCTTTCTTCCGTGAACTGGACAAGCAGCACCGCGAGGAACAGTCCTCCGACGAGCTGTCCGAGCTGATCACCGCTCCTGCTGACGAACTGTACCACGTCGAGGGCACCGTGATCCTGCACATCGTGTTCGCTATCAAGCTGGACTGCGAGCTGGGTCGCGAGCTGCTGAAGCACCTGAAGGCTGGTCAACAGGGCGACCCCTCCAAGTGCCTGTGCCCCTTCTCTATCGCTCTGCTGCTGTCCCTGACCCGTATCCAGCGTTTCGAAGAACAGGTGTTCGACCTGCTCAAGACCTCCGTCGTGAAGTCCTTCAAGGACCTGCAGCTCCTGCAGGGTTCCAAGTTCCTCCAGACTCTGGTGCCCCAGAGGACCTGCGTGTCCACCATGATCCTGGAAGTCGTGCGCAACTCCGTGCACTCCTGGGACCACGTGACCCAGGGCCTGATCGAGTTCGGTTTCATCCTGATGGACTCCTACGGTCCCAAGAAGATCCTCGACGGCAAGGCTGTCGAGATCGGCACCTCCCTGTCCAAGATGACCAACCAGCACGCCTGCAAGCTGGGTGCTAACATCCTCCTGGAAACCTTCAAGATCCACGAGATGATCCGCCAAGAAATCCTGGAACAGGTGCTGAACCGTGTCGTGACCCGTACCTCCTCCCCTATCAACCACTTCCTGGACCTGTTCTCCGACATCATTATGTACGCTCCTCTGATCCTCCAGAACTGCTCCAAAGTGACCGAGACTTTCGACTACCTGACCTTCCTGCCTCTGCAGACCGTGCAGGGACTGCTGAAGGCCGTGCAGCCCCTGCTGAAGATCTCCATGTCCATGCGCGACTCCCTGATCCTGGTGCTGCGCAAGGCTATGTTCGCTTCCCAGCTGGACGCTCGCAAGTCCGCTGTGGCTGGTTTCCTGCTGCTCCTGAAGAACTTCAAGGTGCTGGGTTCCCTGCCATCCTCCCAGTGCACCCAGTCCATCGGTGTCACCCAAGTGCGCGTGGACGTGCACTCTCGTTACTCCGCTGTCGCTAACGAAACCTTCTGCCTGGAAATCATCGACTCCCTCAAGCGTTCCCTGGGCCAGCAGGCTGACATCCGTCTGATGCTGTACGACGGTTTCTACGACGTGCTGCGTCGTAACTCTCAGCTGGCTTCCTCCATCATGCAGACTCTGTTCTCCCAGCTCAAGCAGTTCTACGAGCCCGAGCCCGACCTGCTGCCTCCTCTGAAGTTGGGAGCTTGCGTGCTGACTCAGGGTTCCCAGATCTTCCTGCAAGAACCCCTGGACCACCTCCTGTCCTGCATCCAACACTGCCTGGCTTGGTACAAGTCCCGTGTGGTGCCCCTGCAGCAAGGCGACGAGGGCGAGGAAGAGGAAGAAGAACTGTACTCCGAGCTGGACGACATGCTGGAATCCATCACCGTGCGTATGATCAAGTCCGAGTTGGAGGACTTCGAGTTGGACAAGTCCGCCGACTTCTCCCAGAACACCAACGTGGGCATCAAGAACAACATCTGCGCTTGCCTGATCATGGGCGTGTGCGAGGTGCTGATGGAGTACAACTTCTCCATCTCGAACTTCAGCAAGTCCAAGTTCGAAGAGATCCTGAGCCTGTTCACTTGCTACAAGAAGTTCAGCGACATCCTGTCCGAGAAGGCTGGCAAGGGCAAGGCTAAGATGACCTCCAAGGTGTCCGACTCTCTGCTGTCTCTCAAGTTCGTGTCTGACCTGCTGACCGCCCTGTTCCGTGACTCCATCCAGTCCCACGAGGAATCCCTGTCCGTGCTGAGATCCTCCGGCGAGTTCATGCACTACGCTGTGAACGTGACCCTCCAGAAGATCCAGCAGCTCATCCGTACCGGTCACGTGTCCGGACCTGACGGCCAGAACCCCGACAAGATCTTCCAGAACCTGTGCGACATCACCCGTGTGCTGCTGTGGCGTTACACCTCCATCCCCACCTCCGTGGAAGAGTCCGGAAAGAAGGAAAAGGGCAAGTCCATCTCCCTGCTGTGCCTGGAAGGCCTGCAAAAGACCTTCTCCGTGGTGCTCCAGTTCTACCAACCCAAGGTGCAGCAATTCCTGCAGGCTCTGGACGTGATGGGTACTGAAGAGGAAGAGGCTGGCGTCACCGTGACCCAGAGGGCTAGCTTCCAGATCCGTCAGTTCCAGCGTTCTCTGCTCAACCTGCTGTCCAGCGAAGAGGATGACTTCAACTCCAAGGAAGCCCTGCTGCTGATCGCCGTGCTGTCCACCCTGTCCCGTCTGCTGGAACCTACCTCCCCACAGTTCGTGCAGATGCTGTCCTGGACTTCCAAGATCTGCAAGGAATACTCTCAAGAGGACGCCAGCTTCTGCAAGTCTCTGATGAACCTCTTCTTCTCCCTCCACGTGCTGTACAAGTCTCCCGTGACCCTGCTGAGGGACCTGTCCCAGGACATCCACGGTCAACTGGGAGACATCGACCAGGACGTGGAAATCGAAAAGACCGACCACTTCGCTGTGGTCAACCTGCGTACCGCTGCTCCCACTGTGTGCCTGCTGGTGTTGTCCCAGGCCGAGAAGGTCCTGGAAGAGGTCGACTGGCTGATCGCTAAGATCAAGGGTTCCGCTAACCAGGAAACCCTGTCTGACAAAGTGACTCCCGAGGACGCCTCCTCCCAGGCTGTTCCTCCTACCCTGCTCATCGAGAAGGCCATCGTGATGCAGCTGGGTACTCTGGTCACCTTCTTCCACGAACTGGTGCAGACTGCTCTGCCCTCCGGTTCCTGCGTGGACACCCTCCTGAAGGGCCTGTCTAAGATCTACAGCACCCTGACCGCCTTCGTGAAGTACTACCTGCAAGTGTGCCAGTCCTCCCGTGGTATCCCCAACACCGTCGAGAAGCTGGTCAAGCTGTCCGGTTCCCACCTGACCCCTGTGTGCTACTCCTTCATCTCCTACGTGCAGAACAAGTCCTCTGACGCTCCCAAGTGCAGCGAAAAGGAAAAGGCTGCTGTCTCCACCACAATGGCTAAGGTCCTGCGCGAGACTAAGCCCATCCCCAACCTGGTGTTCGCCATCGAGCAGTACGAGAAGTTCCTGATCCAGTTGTCCAAGAAGTCCAAAGTCAACCTGATGCAGCACATGAAGCTGTCCACTTCCCGCGATTTCAAGATCAAGGGCAGCGTGCTGGACATGGTGCTCCGCGAGGATGAGCATCATCACCACCACCACTAAGGTACC

Amino Acid Sequence

MDLKILSLATDKTTDKLQEFLQTLKDDDLASLLQNQAVKGRAVGTLLRAVLKGSPCSEEDGALRRYKIYSCCIQLVESGDLQQDVASEIIGLLMLEVHHF
PGPLLVDLASDFVGAVREDRLVNGKSLELLPIILTALATKKEVLACGKGDLNGEEYKRQLIDTLCSVRWPQRYMIQLTSVFKDVCLTPEEMNLVVAKVLT
MFSKLNLQEIPPLVYQLLVLSSKGSRRSVLDGIIAFFRELDKQHREEQSSDELSELITAPADELYHVEGTVILHIVFAIKLDCELGRELLKHLKAGQQGD
PSKCLCPFSIALLLSLTRIQRFEEQVFDLLKTSVVKSFKDLQLLQGSKFLQTLVPQRTCVSTMILEVVRNSVHSWDHVTQGLIEFGFILMDSYGPKKILD
GKAVEIGTSLSKMTNQHACKLGANILLETFKIHEMIRQEILEQVLNRVVTRTSSPINHFLDLFSDIIMYAPLILQNCSKVTETFDYLTFLPLQTVQGLLK
AVQPLLKISMSMRDSLILVLRKAMFASQLDARKSAVAGFLLLLKNFKVLGSLPSSQCTQSIGVTQVRVDVHSRYSAVANETFCLEIIDSLKRSLGQQADI
RLMLYDGFYDVLRRNSQLASSIMQTLFSQLKQFYEPEPDLLPPLKLGACVLTQGSQIFLQEPLDHLLSCIQHCLAWYKSRVVPLQQGDEGEEEEEELYSE
LDDMLESITVRMIKSELEDFELDKSADFSQNTNVGIKNNICACLIMGVCEVLMEYNFSISNFSKSKFEEILSLFTCYKKFSDILSEKAGKGKAKMTSKVS
DSLLSLKFVSDLLTALFRDSIQSHEESLSVLRSSGEFMHYAVNVTLQKIQQLIRTGHVSGPDGQNPDKIFQNLCDITRVLLWRYTSIPTSVEESGKKEKG
KSISLLCLEGLQKTFSVVLQFYQPKVQQFLQALDVMGTEEEEAGVTVTQRASFQIRQFQRSLLNLLSSEEDDFNSKEALLLIAVLSTLSRLLEPTSPQFV
QMLSWTSKICKEYSQEDASFCKSLMNLFFSLHVLYKSPVTLLRDLSQDIHGQLGDIDQDVEIEKTDHFAVVNLRTAAPTVCLLVLSQAEKVLEEVDWLIA
KIKGSANQETLSDKVTPEDASSQAVPPTLLIEKAIVMQLGTLVTFFHELVQTALPSGSCVDTLLKGLSKIYSTLTAFVKYYLQVCQSSRGIPNTVEKLVK
LSGSHLTPVCYSFISYVQNKSSDAPKCSEKEKAAVSTTMAKVLRETKPIPNLVFAIEQYEKFLIQLSKKSKVNLMQHMKLSTSRDFKIKGSVLDMVLRED
EHHHHHH*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU50859
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee