Huwe1

• Synonyms: ARF-BP1; HECTH9; HSPC272; Ib772; LASU1; MULE; URE-B1; UREB1
• Plasmid: pCAGGS puro HA Huwe1 K3551-L4373
• Parent Plasmid: pCAGGS puro HA
• Expressed: HA Huwe1 K3551-L4373
• Genbank: NM_031407.6 GI:531034782 with 2 silent changes and residue change V3765A
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCAAGGGCAGCAAATCTCCAGCGAAGGTGAGTGATGGGGGCAGCAGCAGTACAGACTTTAAGATGGTGTCCTCTGGCCTCACTGAAAACCAGCTACAGCTCTCTGTAGAGGTGTTGACATCCCACTCTTGTTCTGAGGAAGGCTTAGAGGATGCAGCCAACGTACTACTGCAGCTCTCCCGGGGGGACTCTGGGACCCGGGACACTGTTCTCAAGCTGCTACTGAATGGAGCCCGCCATCTGGGTTATACCCTTTGTAAACAAATAGGTACCCTGCTGGCCGAGCTGCGGGAATACAACCTCGAGCAGCAGCGGCGAGCCCAATGTGAAACCCTCTCTCCTGATGGCCTGCCTGAGGAGCAGCCACAGACCACCAAGCTGAAGGGCAAAATGCAGAGCAGGTTTGACATGGCTGAGAATGTGGTAATTGTGGCATCTCAGAAGCGACCTTTGGGTGGCCGGGAGCTCCAGCTGCCTTCTATGTCCATGTTGACATCCAAGACATCTACCCAGAAGTTCTTCTTGAGGGTACTACAGGTCATCATCCAGCTCCGGGACGACACGCGCCGGGCTAACAAGAAAGCCAAGCAGACAGGCAGGCTAGGTTCCTCCGGTTTAGGCTCAGCTAGCAGCATCCAGGCAGCTGCTCGGCAGCTGGAGGCTGAGGCTGATGCCATTATACAAATGGTACGTGAGGGTCAAAGGGCGCGGAGACAGCAACAAGCAGCAACGTCGGAGTCTAGCCAGTCAGAGGCGTCTGTCCGGAGGGAGGAATCACCCATGGATGTGGACCAGCCATCTCCCAGTGCTCAAGATACTCAATCCATTGCCTCCGATGGAACCCCACAGGGGGAGAAGGAAAAGGAAGAAAGACCACCTGAGTTACCCCTGCTCAGCGAGCAGCTGAGTTTGGACGAGCTGTGGGACATGCTTGGGGAGTGTCTAAAGGAACTAGAGGAATCCCATGACCAGCATGCGGTGCTAGTGCTACAGCCTGCTGTCGAGGCCTTCTTTCTGGTCCATGCCACAGAGCGGGAGAGCAAGCCTCCTGTCCGAGACACCCGTGAGAGCCAGCTGGCACACATCAAGGACGAGCCTCCTCCACTCTCCCCTGCCCCCTTAACCCCAGCCACGCCTTCCTCCCTTGACCCATTCTTCTCCCGGGAGCCCTCATCTATGCACATCTCCTCAAGCCTGCCCCCTGACACACAGAAGTTCCTTCGCTTTGCAGAGACTCACCGCACTGTGTTAAACCAGATCCTACGGCAGTCCACGACCCACCTTGCTGATGGGCCTTTTGCTGTCCTGGTAGACTACATTCGTGTCCTCGACTTTGATGTCAAGCGCAAATATTTCCGCCAAGAGCTGGAGCGTTTAGATGAGGGGCTCCGGAAAGAAGACATGGCTGTGCATGTCCGTCGTGACCATGTGTTTGAAGACTCCTATCGTGAGCTGCATCGCAAATCCCCCGAAGAAATGAAGAATCGATTGTATATAGTATTTGAAGGAGAAGAAGGGCAGGATGCTGGCGGGCTCCTGCGGGAGTGGTATATGATCATCTCTCGAGAGATGTTTAACCCTATGTATGCCTTGTTCCGTACCTCACCTGGTGATCGAGTCACCTACACCATCAATCCATCTTCCCACTGCAACCCCAACCACCTCAGCTACTTCAAGTTTGTCGGACGCATTGTGGCCAAAGCTGTATATGACAACCGTCTTCTGGAGTGCTACTTTACTCGATCCTTTTACAAACACATCTTGGGCAAGTCAGTCAGATATACAGATATGGAGAGTGAAGATTACCACTTCTACCAAGGTCTGGTTTATCTGCTGGAAAATGATGTCTCCACACTAGGCTATGACCTCACCTTCAGCACTGAGGTCCAAGAGTTTGGAGTTTGTGAAGTTCGTGACCTCAAACCCAATGGGGCCAACATCTTGGTAACAGAGGAGAATAAGAAGGAGTATGTACACCTGGTATGCCAGATGAGAATGACAGGAGCCATCCGCAAGCAGTTGGCGGCTTTCTTAGAAGGCTTCTATGAGATCATTCCAAAGCGCCTCATTTCCATCTTCACTGAGCAGGAGTTAGAGCTGCTTATATCAGGACTGCCCACCATTGACATCGATGATCTGAAATCCAACACTGAATACCACAAGTACCAGTCCAACTCTATTCAGATCCAGTGGTTCTGGAGAGCATTGCGTTCTTTCGATCAAGCTGACCGTGCCAAGTTCCTCCAGTTTGTCACGGGTACTTCCAAGGTACCCCTGCAAGGCTTTGCTGCCCTCGAAGGCATGAATGGCATTCAGAAGTTTCAGATCCATCGAGATGACAGGTCCACAGATCGCCTGCCTTCAGCTCACACATGTTTTAATCAGCTGGATCTGCCTGCCTATGAGAGCTTTGAGAAGCTCCGCCACATGCTACTGTTGGCTATCCAGGAGTGCTCTGAAGGCTTTGGGCTGTAATCGCGA

Amino Acid Sequence

MYPYDVPDYAGSKGSKSPAKVSDGGSSSTDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQI
GTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTR
RANKKAKQTGRLGSSGLGSASSIQAAARQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKE
ERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHATERESKPPVRDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSRE
PSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKS
PEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILG
KSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRL
ISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSA
HTCFNQLDLPAYESFEKLRHMLLLAIQECSEGFGL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU50792
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee