N4BP1

• Plasmid: pGEX C3 HALO thrombin-N4BP1 Lys296-Asp896
• Parent Plasmid: pSC-B
• Expressed: GST-HALO-N4BP1 Lys296-Asp896
• Genbank: NM_153029.3
• Tag: HALO
• Tag: GST
• Species: Human

Sequence of Insert

ggatccAAGAAGCAGTTTTCTTTGGAAAATGTTCAGGAGGGGGAGATTTT
ACACGATGCTAAGACATTGGCTGGAAATGTAATAGCTGACCTATCTGATT
CTTCTGCTGATTCTGAAAATTTAAGTCCAGATATAAAAGAAACTACTGAG
GAAATGGAATACAACATCCTCGTAAACTTTTTTAAAACCATGGGCTACTC
CCAAGAAATTGTTGAAAAGGTCATTAAGGTGTATGGACCATCTACTGAAC
CATTATTGCTCTTAGAGGAAATTGAAAAAGAAAATAAAAGATTCCAAGAA
GACAGAGAATTTTCAGCTGGTACAGTGTATCCAGAGACCAACAAAACCAA
AAATAAAGGTGTTTATAGCAGCACAAATGAGCTTACAACTGATTCCACTC
CAAAGAAAACACAGGCTCACACACAGCAAAATATGGTAGAAAAATTTTCT
CAGTTACCATTCAAAGTGGAAGCTAAACCATGTACCTCAAATTGTAGAAT
TAATACTTTCAGAACAGTGCCAATAGAACAGAAACATGAAGTCTGGGGTT
CAAACCAGAACTACATTTGTAACACAGACCCTGAAACTGATGGCCTTTCA
CCTTCTGTTGCCTCTCCAAGTCCCAAAGAAGTCAATTTTGTTTCAAGGGG
AGCTTCAAGTCACCAGCCCAGAGTTCCACTTTTTCCTGAAAATGGTTTAC
ACCAGCAGCCAGAACCCTTGCTTCCAAATAATATGAAATCTGCCTGTGAA
AAACGTTTAGGATGTTGTAGTTCTCCTCATTCTAAGCCAAATTGCTCAAC
CCTTTCTCCACCAATGCCACTGCCCCAGCTGTTACCTTCGGTTACTGATG
CAAGGTCGGCAGGACCTTCTGATCATATTGATTCCTCAGTTACTGGGGTT
CAAAGGTTTCGAGATACTCTAAAAATACCCTACAAGCTGGAATTAAAAAA
TGAACCAGGGAGAACGGATTTGAAACACATTGTTATAGATGGGAGCAATG
TTGCAATTACCCATGGTCTGAAAAAGTTCTTTTCTTGTCGTGGAATTGCA
ATTGCAGTTGAATATTTTTGGAAGCTTGGCAACAGAAACATCACTGTATT
TGTCCCTCAGTGGAGAACAAGGCGTGATCCTAATGTCACAGAACAGCACT
TCTTAACCCAGCTCCAGGAGCTCGGAATATTATCTTTAACTCCTGCCCGG
ATGGTCTTTGGAGAAAGAATTGCTTCTCATGATGACAGGTTTCTACTACA
CTTAGCGGACAAAACTGGTGGCATAATTGTGACAAATGATAATTTCAGAG
AATTTGTGAATGAGTCAGTCTCTTGGAGAGAAATTATTACAAAAAGGCTG
CTGCAGTACACGTTCGTGGGGGACATATTTATGGTTCCCGATGATCCTCT
GGGAAGAAGTGGACCTCGATTAGAGGAGTTTCTTCAGAAGGAAGTCTGTC
TTAGAGATATGCAGCCCCTACTCAGTGCCCTGCCAAATGTGGGCATGTTT
GACCCCAGCTTCAGAGTCCCTGGCACCCAGGCAGCCAGCACCAGCCACCA
GCCTCCGACCCGGATTCAGGGAGCCCCTTCAAGCCACTGGCTCCCTCAGC
AGCCCCACTTCCCACTGCTGCCAGCCCTCCCCAGTCTCCAGCAGAACCTG
CCCATGCCAGCTCAGAGATCTTCTGCAGAAACCAACGAGCTGAGGGAAGC
CCTTCTGAAGATCTTCCCTGACTCAGAGCAAAGACTGAAAATAGACCAGA
TCTTGGTGGCCCACCCATACATGAAAGATCTAAATGCGCTTTCTGCCATG
GTGTTGGATTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSKKQFSLENVQEGEI
LHDAKTLAGNVIADLSDSSADSENLSPDIKETTEEMEYNILVNFFKTMGY
SQEIVEKVIKVYGPSTEPLLLLEEIEKENKRFQEDREFSAGTVYPETNKT
KNKGVYSSTNELTTDSTPKKTQAHTQQNMVEKFSQLPFKVEAKPCTSNCR
INTFRTVPIEQKHEVWGSNQNYICNTDPETDGLSPSVASPSPKEVNFVSR
GASSHQPRVPLFPENGLHQQPEPLLPNNMKSACEKRLGCCSSPHSKPNCS
TLSPPMPLPQLLPSVTDARSAGPSDHIDSSVTGVQRFRDTLKIPYKLELK
NEPGRTDLKHIVIDGSNVAITHGLKKFFSCRGIAIAVEYFWKLGNRNITV
FVPQWRTRRDPNVTEQHFLTQLQELGILSLTPARMVFGERIASHDDRFLL
HLADKTGGIIVTNDNFREFVNESVSWREIITKRLLQYTFVGDIFMVPDDP
LGRSGPRLEEFLQKEVCLRDMQPLLSALPNVGMFDPSFRVPGTQAASTSH
QPPTRIQGAPSSHWLPQQPHFPLLPALPSLQQNLPMPAQRSSAETNELRE
ALLKIFPDSEQRLKIDQILVAHPYMKDLNALSAMVLD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24990
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee