USP48

• Plasmid: pcDNA-FRT/TO-GFP-USP48 tv1 C98A
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-USP48 tv1 C98A
• Genbank: NM_032236.5
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGCCCCGCGGCTGCAGCTGGAGAAGGCGGCCTGGCGCTGGGC
GGAGACGGTGCGGCCCGAGGAGGTGTCGCAGGAGCACATCGAGACCGCTT
ACCGCATCTGGCTGGAGCCCTGCATTCGCGGCGTGTGCAGACGAAACTGC
AAAGGAAATCCGAATTGCTTGGTTGGTATTGGTGAGCATATTTGGTTAGG
AGAAATAGATGAAAATAGTTTTCATAACATCGATGATCCCAACTGTGAGA
GGAGAAAAAAGAACTCATTTGTGGGCCTGACTAACCTTGGAGCCACTgcc
TATGTCAACACATTTCTTCAAGTGTGGTTTCTCAACTTGGAGCTTCGGCA
GGCACTCTACTTATGTCCAAGCACTTGTAGTGACTACATGCTGGGAGACG
GCATCCAAGAAGAAAAAGATTATGAGCCTCAAACAATTTGTGAGCATCTC
CAGTACTTGTTTGCCTTGTTGCAAAACAGTAATAGGCGATACATTGATCC
ATCAGGATTTGTTAAAGCCTTGGGCCTGGACACTGGACAACAGCAGGATG
CTCAAGAATTTTCAAAGCTCTTTATGTCTCTATTGGAAGATACTTTGTCT
AAACAAAAGAATCCAGATGTGCGCAATATTGTTCAACAGCAGTTCTGTGG
AGAATATGCCTATGTAACTGTTTGCAACCAGTGTGGCAGAGAGTCTAAGC
TTTTGTCAAAATTTTATGAGCTGGAGTTAAATATCCAAGGCCACAAACAG
TTAACAGATTGTATCTCGGAATTTTTGAAGGAAGAAAAATTAGAAGGAGA
CAATCGCTATTTTTGCGAGAACTGTCAAAGCAAACAGAATGCAACAAGAA
AGATTCGACTTCTTAGCCTTCCTTGCACTCTGAACTTGCAGCTAATGCGT
TTTGTCTTTGACAGGCAAACTGGACATAAGAAAAAGCTGAATACCTACAT
TGGCTTCTCAGAAATTTTGGATATGGAGCCTTATGTGGAACATAAAGGTG
GGTCCTACGTGTATGAACTCAGCGCAGTCCTCATACACAGAGGAGTGAGT
GCTTATTCTGGCCACTACATCGCCCACGTGAAAGATCCACAGTCTGGTGA
ATGGTATAAGTTTAATGATGAAGACATAGAAAAGATGGAGGGGAAGAAAT
TACAACTAGGGATTGAGGAAGATCTAGCAGAACCTTCTAAGTCTCAGACA
CGTAAACCCAAGTGTGGCAAAGGAACTCATTGCTCTCGAAATGCATATAT
GTTGGTTTATAGACTGCAAACTCAAGAAAAGCCCAACACTACTGTTCAAG
TTCCAGCCTTTCTTCAAGAGCTGGTAGATCGGGATAATTCCAAATTTGAG
GAGTGGTGTATTGAAATGGCTGAGATGCGTAAGCAAAGTGTGGATAAAGG
AAAAGCAAAACACGAAGAGGTTAAGGAGCTGTACCAAAGGTTACCTGCTG
GAGCTGAGCCCTATGAGTTTGTCTCTCTGGAATGGCTGCAAAAGTGGTTG
GATGAATCAACACCTACCAAACCTATTGATAATCACGCTTGCCTGTGTTC
CCATGACAAGCTTCACCCGGATAAAATATCAATTATGAAGAGGATATCTG
AATATGCAGCTGACATTTTCTATAGTAGATATGGAGGAGGTCCAAGACTA
ACTGTGAAAGCCCTGTGTAAGGAATGTGTAGTAGAACGTTGTCGCATATT
GCGTCTGAAGAACCAACTAAATGAAGATTATAAAACTGTTAATAATCTGC
TGAAAGCAGCAGTAAAGGGCAGCGATGGATTTTGGGTGGGGAAGTCCTCC
TTGCGGAGTTGGCGCCAGCTAGCTCTTGAACAGCTGGATGAGCAAGATGG
TGATGCAGAACAAAGCAACGGAAAGATGAACGGTAGCACCTTAAATAAAG
ATGAATCAAAGGAAGAAAGAAAAGAAGAGGAGGAATTAAATTTTAATGAA
GATATTCTGTGTCCACATGGTGAGTTATGCATATCTGAAAATGAAAGAAG
GCTTGTTTCTAAAGAGGCTTGGAGCAAACTGCAGCAGTACTTTCCAAAGG
CTCCTGAGTTTCCAAGTTACAAAGAGTGCTGTTCACAGTGCAAGATTTTA
GAAAGAGAAGGGGAAGAAAATGAAGCCTTACATAAGATGATTGCAAACGA
GCAAAAGACTTCTCTCCCAAATTTGTTCCAGGATAAAAACAGACCGTGTC
TCAGTAACTGGCCAGAGGATACGGATGTCCTCTACATCGTGTCTCAGTTC
TTTGTAGAAGAGTGGCGGAAATTTGTTAGAAAGCCTACAAGATGCAGCCC
TGTGTCATCAGTTGGGAACAGTGCTCTTTTGTGTCCCCACGGGGGCCTCA
TGTTTACATTTGCTTCCATGACCAAAGAAGATTCTAAACTTATAGCTCTC
ATATGGCCCAGTGAGTGGCAAATGATACAAAAGCTCTTTGTTGTGGATCA
TGTAATTAAAATCACGAGAATTGAAGTGGGAGATGTAAACCCTTCAGAAA
CACAGTATATTTCTGAGCCCAAACTCTGTCCAGAATGCAGAGAAGGCTTA
TTGTGTCAGCAGCAGAGGGACCTGCGTGAATACACTCAAGCCACCATCTA
TGTCCATAAAGTTGTGGATAATAAAAAGGTGATGAAGGATTCGGCTCCGG
AACTGAATGTGAGTAGTTCTGAAACAGAGGAGGACAAGGAAGAAGCTAAA
CCAGATGGAGAAAAAGATCCAGATTTTAATCAAAGCAATGGTGGAACAAA
GCGGCAAAAGATATCCCATCAAAATTATATAGCCTATCAAAAGCAAGTTA
TTCGCCGAAGTATGCGACATAGAAAAGTTCGTGGTGAGAAAGCACTTCTC
GTTTCTGCTAATCAGACGTTAAAAGAATTGAAAATTCAGATCATGCATGC
ATTTTCAGTTGCTCCTTTTGACCAGAATTTGTCAATTGATGGAAAGATTT
TAAGTGATGACTGTGCCACCCTAGGCACCCTTGGCGTCATTCCTGAATCT
GTCATTTTATTGAAGGCTGATGAACCAATTGCAGATTATGCTGCAATGGA
TGATGTCATGCAAGTTTGTATGCCAGAAGAAGGGTTTAAAGGTACTGGTC
TTCTTGGACATTAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMAPRLQ
LEKAAWRWAETVRPEEVSQEHIETAYRIWLEPCIRGVCRRNCKGNPNCLV
GIGEHIWLGEIDENSFHNIDDPNCERRKKNSFVGLTNLGATAYVNTFLQV
WFLNLELRQALYLCPSTCSDYMLGDGIQEEKDYEPQTICEHLQYLFALLQ
NSNRRYIDPSGFVKALGLDTGQQQDAQEFSKLFMSLLEDTLSKQKNPDVR
NIVQQQFCGEYAYVTVCNQCGRESKLLSKFYELELNIQGHKQLTDCISEF
LKEEKLEGDNRYFCENCQSKQNATRKIRLLSLPCTLNLQLMRFVFDRQTG
HKKKLNTYIGFSEILDMEPYVEHKGGSYVYELSAVLIHRGVSAYSGHYIA
HVKDPQSGEWYKFNDEDIEKMEGKKLQLGIEEDLAEPSKSQTRKPKCGKG
THCSRNAYMLVYRLQTQEKPNTTVQVPAFLQELVDRDNSKFEEWCIEMAE
MRKQSVDKGKAKHEEVKELYQRLPAGAEPYEFVSLEWLQKWLDESTPTKP
IDNHACLCSHDKLHPDKISIMKRISEYAADIFYSRYGGGPRLTVKALCKE
CVVERCRILRLKNQLNEDYKTVNNLLKAAVKGSDGFWVGKSSLRSWRQLA
LEQLDEQDGDAEQSNGKMNGSTLNKDESKEERKEEEELNFNEDILCPHGE
LCISENERRLVSKEAWSKLQQYFPKAPEFPSYKECCSQCKILEREGEENE
ALHKMIANEQKTSLPNLFQDKNRPCLSNWPEDTDVLYIVSQFFVEEWRKF
VRKPTRCSPVSSVGNSALLCPHGGLMFTFASMTKEDSKLIALIWPSEWQM
IQKLFVVDHVIKITRIEVGDVNPSETQYISEPKLCPECREGLLCQQQRDL
REYTQATIYVHKVVDNKKVMKDSAPELNVSSSETEEDKEEAKPDGEKDPD
FNQSNGGTKRQKISHQNYIAYQKQVIRRSMRHRKVRGEKALLVSANQTLK
ELKIQIMHAFSVAPFDQNLSIDGKILSDDCATLGTLGVIPESVILLKADE
PIADYAAMDDVMQVCMPEEGFKGTGLLGH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24839
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee