USP42

• Plasmid: pcDNA5D FRT/TO BirA R118G-USP42 C120A-HA
• Parent Plasmid: pcDNA5D FRT/TO BirA R118G MCS HA
• Expressed: BirA R118G-USP42 C120A-HA
• Genbank: NM_032172.2
• Tag: HA
• Tag: BirA
• Species: Human

Sequence of Insert

GGATCCATGACCATAGTTGACAAAGCTTCTGAATCTTCAGACCCATCAGC
CTATCAGAATCAGCCTGGCAGCTCCGAGGCAGTCTCACCTGGAGACATGG
ATGCAGGTTCTGCCAGCTGGGGTGCTGTGTCTTCATTGAATGATGTGTCA
AATCACACACTTTCTTTAGGACCAGTACCTGGTGCTGTAGTTTATTCGAG
TTCATCTGTACCTGATAAATCAAAACCATCACCACAAAAGGATCAAGCCC
TAGGTGATGGCATCGCTCCTCCACAGAAAGTTCTTTTCCCATCTGAGAAG
ATTTGTCTTAAGTGGCAACAAACTCATAGAGTTGGAGCTGGGCTCCAGAA
TTTGGGCAATACCgcTTTTGCCAATGCAGCACTGCAGTGTTTAACCTACA
CACCACCTCTTGCCAATTACATGCTATCACATGAACACTCCAAAACATGT
CATGCAGAAGGCTTTTGTATGATGTGTACAATGCAAGCACATATTACCCA
GGCACTCAGTAATCCTGGGGACGTTATTAAACCAATGTTTGTCATCAATG
AGATGCGGCGTATAGCTAGGCACTTCCGTTTTGGAAACCAAGAAGATGCC
CATGAATTCCTTCAATACACTGTTGATGCTATGCAGAAAGCATGCTTGAA
TGGCAGCAATAAATTAGACAGACACACCCAGGCCACCACTCTTGTTTGTC
AGATATTTGGAGGATACCTAAGATCTAGAGTCAAATGTTTAAATTGCAAG
GGCGTTTCAGATACTTTTGATCCATATCTTGATATAACATTGGAGATAAA
GGCTGCTCAGAGTGTCAACAAGGCATTGGAGCAGTTTGTGAAGCCGGAAC
AGCTTGATGGAGAAAACTCGTACAAGTGCAGCAAGTGTAAAAAGATGGTT
CCAGCTTCAAAGAGGTTCACTATCCATAGATCCTCTAATGTTCTTACACT
TTCTCTGAAACGTTTTGCAAATTTTACCGGTGGAAAAATTGCTAAGGATG
TGAAATACCCTGAGTATCTTGATATTCGGCCATATATGTCTCAACCCAAC
GGAGAGCCAATTGTCTACGTCTTGTATGCAGTGCTGGTCCACACTGGTTT
TAATTGCCATGCTGGCCATTACTTCTGCTACATAAAAGCTAGCAATGGCC
TCTGGTATCAAATGAATGACTCCATTGTATCTACCAGTGATATTAGATCG
GTACTCAGCCAACAAGCCTATGTGCTCTTTTATATCAGGTCCCATGATGT
GAAAAATGGAGGTGAACTTACTCATCCCACCCATAGCCCCGGCCAGTCCT
CTCCCCGCCCCGTCATCAGTCAGCGGGTTGTCACCAACAAACAGGCTGCG
CCAGGCTTTATCGGACCACAGCTTCCCTCTCACATGATAAAGAATCCACC
TCACTTAAATGGGACTGGACCATTGAAAGACACGCCAAGCAGTTCCATGT
CGAGTCCTAACGGGAATTCCAGTGTCAACAGGGCTAGTCCTGTTAATGCT
TCAGCTTCTGTCCAAAACTGGTCAGTTAATAGGTCCTCAGTGATCCCAGA
ACATCCTAAGAAACAAAAAATTACAATCAGTATTCACAACAAGTTGCCTG
TTCGCCAGTGTCAGTCTCAACCTAACCTTCATAGTAATTCTTTGGAGAAC
CCTACCAAGCCCGTTCCCTCTTCTACCATTACCAATTCTGCAGTACAGTC
TACCTCGAACGCATCTACGATGTCAGTTTCTAGTAAAGTAACAAAACCGA
TCCCCCGCAGTGAATCCTGCTCCCAGCCCGTGATGAATGGCAAATCCAAG
CTGAACTCCAGCGTGCTGGTGCCCTATGGCGCCGAGTCCTCTGAGGACTC
TGACGAGGAGTCAAAGGGGCTGGGCAAGGAGAATGGGATTGGTACGATTG
TGAGCTCCCACTCTCCCGGCCAAGATGCCGAAGATGAGGAGGCCACTCCG
CACGAGCTTCAAGAACCCATGACCCTAAACGGTGCTAATAGTGCAGACAG
CGACAGTGACCCGAAAGAAAACGGCCTAGCGCCTGATGGTGCCAGCTGCC
AAGGCCAGCCTGCCCTGCACTCAGAAAATCCCTTTGCTAAGGCAAACGGT
CTTCCTGGAAAGTTGATGCCTGCTCCTTTGCTGTCTCTCCCAGAAGACAA
AATCTTAGAGACCTTCAGGCTTAGCAACAAACTGAAAGGCTCGACGGATG
AAATGAGTGCACCTGGAGCAGAGAGGGGCCCTCCCGAGGACCGCGACGCC
GAGCCTCAGCCTGGCAGCCCCGCCGCCGAATCCCTGGAGGAGCCAGATGC
GGCCGCCGGCCTCAGCAGCACCAAGAAGGCTCCGCCGCCCCGCGATCCCG
GCACCCCCGCTACCAAAGAAGGCGCCTGGGAGGCCATGGCCGTCGCCCCC
GAGGAGCCTCCGCCCAGCGCCGGCGAGGACATCGTGGGGGACACAGCACC
CCCTGACCTGTGTGATCCCGGGAGCTTAACAGGCGATGCGAGCCCGTTGT
CCCAGGACGCAAAGGGGATGATCGCGGAGGGCCCGCGGGACTCGGCGTTG
GCGGAAGCCCCGGAAGGGTTGAGTCCGGCTCCGCCTGCGCGGTCGGAGGA
GCCCTGCGAGCAGCCACTCCTTGTTCACCCCAGCGGGGACCACGCCCGGG
ACGCTCAGGACCCATCCCAGAGCTTGGGCGCACCCGAGGCCGCAGAGCGG
CCGCCAGCTCCTGTGCTGGACATGGCCCCGGCCGGTCACCCGGAAGGGGA
CGCTGAGCCTAGCCCCGGCGAGAGGGTCGAGGACGCCGCGGCGCCGAAAG
CCCCAGGCCCTTCCCCAGCGAAGGAGAAAATCGGCAGCCTCAGAAAGGTG
GACCGAGGCCACTACCGCAGCCGGAGAGAGCGCTCGTCCAGCGGGGAGCC
CGCCAGAGAGAGCAGGAGCAAGACTGAGGGCCACCGTCACCGGCGGCGCC
GCACCTGCCCCCGGGAGCGCGACCGCCAGGACCGCCACGCCCCGGAGCAC
CACCCCGGCCACGGCGACAGGCTCAGCCCTGGCGAGCGCCGCTCTCTGGG
CAGGTGCAGTCACCACCACTCCCGACACCGGAGCGGGGTGGAGCTGGACT
GGGTCAGACACCACTACACCGAGGGCGAGCGTGGCTGGGGCCGGGAGAAG
TTCTACCCCGACAGGCCGCGCTGGGACAGGTGCCGGTACTACCATGACAG
GTACGCCCTGTACGCTGCCCGGGACTGGAAGCCCTTCCACGGCGGCCGCG
AGCACGAGCGGGCCGGGCTGCACGAGCGGCCGCACAAGGACCACAACCGG
GGCCGTAGGGGCTGCGAGCCGGCCCGGGAGAGGGAGCGGCACCGCCCCAG
CAGCCCCCGCGCAGGCGCGCCCCACGCCCTCGCCCCGCACCCCGACCGCT
TCTCCCACGACAGAACTGCACTTGTAGCCGGAGACAACTGTAACCTCTCT
GATCGGTTTCACGAACACGAAAATGGAAAGTCCCGGAAACGGAGACACGA
CAGTGTGGAGAACAGTGACAGTCATGTTGAAAAGAAAGCCCGGAGGAGCG
AACAGAAGGACCCTCTAGAAGAGCCTAAAGCAAAGAAGCACAAAAAATCA
AAGAAGAAAAAGAAATCCAAAGACAAACACCGAGACCGCGACTCCAGGCA
TCAGCAGGACTCAGACCTCTCAGCAGCGTGCTCTGACGCTGACCTCCACA
GACACAAAAAAAAGAAGAAGAAAAAGAAGAGACATTCAAGAAAATCAGAG
GACTTTGTTAAAGATTCAGAACTGCACTTACCCAGGGTCACCAGCTTGGA
GACTGTCGCCCAGTTCCGGAGAGCCCAGGGTGGCTTTCCTCTCTCTGGTG
GCCCGCCTCTGGAAGGCGTCGGACCTTTCCGTGAGAAAACGAAACACTTA
CGGATGGAAAGCAGGGATGACAGGTGTCGTCTCTTTGAGTATGGCCAGGG
TGATCTCGAG

Amino Acid Sequence

MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDV
FTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGE
LKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLS
LVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQ
IVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAAL
ELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEKGSMTIVDKASESSDPSAYQNQPGSSEAVS
PGDMDAGSASWGAVSSLNDVSNHTLSLGPVPGAVVYSSSSVPDKSKPSPQ
KDQALGDGIAPPQKVLFPSEKICLKWQQTHRVGAGLQNLGNTAFANAALQ
CLTYTPPLANYMLSHEHSKTCHAEGFCMMCTMQAHITQALSNPGDVIKPM
FVINEMRRIARHFRFGNQEDAHEFLQYTVDAMQKACLNGSNKLDRHTQAT
TLVCQIFGGYLRSRVKCLNCKGVSDTFDPYLDITLEIKAAQSVNKALEQF
VKPEQLDGENSYKCSKCKKMVPASKRFTIHRSSNVLTLSLKRFANFTGGK
IAKDVKYPEYLDIRPYMSQPNGEPIVYVLYAVLVHTGFNCHAGHYFCYIK
ASNGLWYQMNDSIVSTSDIRSVLSQQAYVLFYIRSHDVKNGGELTHPTHS
PGQSSPRPVISQRVVTNKQAAPGFIGPQLPSHMIKNPPHLNGTGPLKDTP
SSSMSSPNGNSSVNRASPVNASASVQNWSVNRSSVIPEHPKKQKITISIH
NKLPVRQCQSQPNLHSNSLENPTKPVPSSTITNSAVQSTSNASTMSVSSK
VTKPIPRSESCSQPVMNGKSKLNSSVLVPYGAESSEDSDEESKGLGKENG
IGTIVSSHSPGQDAEDEEATPHELQEPMTLNGANSADSDSDPKENGLAPD
GASCQGQPALHSENPFAKANGLPGKLMPAPLLSLPEDKILETFRLSNKLK
GSTDEMSAPGAERGPPEDRDAEPQPGSPAAESLEEPDAAAGLSSTKKAPP
PRDPGTPATKEGAWEAMAVAPEEPPPSAGEDIVGDTAPPDLCDPGSLTGD
ASPLSQDAKGMIAEGPRDSALAEAPEGLSPAPPARSEEPCEQPLLVHPSG
DHARDAQDPSQSLGAPEAAERPPAPVLDMAPAGHPEGDAEPSPGERVEDA
AAPKAPGPSPAKEKIGSLRKVDRGHYRSRRERSSSGEPARESRSKTEGHR
HRRRRTCPRERDRQDRHAPEHHPGHGDRLSPGERRSLGRCSHHHSRHRSG
VELDWVRHHYTEGERGWGREKFYPDRPRWDRCRYYHDRYALYAARDWKPF
HGGREHERAGLHERPHKDHNRGRRGCEPARERERHRPSSPRAGAPHALAP
HPDRFSHDRTALVAGDNCNLSDRFHEHENGKSRKRRHDSVENSDSHVEKK
ARRSEQKDPLEEPKAKKHKKSKKKKKSKDKHRDRDSRHQQDSDLSAACSD
ADLHRHKKKKKKKKRHSRKSEDFVKDSELHLPRVTSLETVAQFRRAQGGF
PLSGGPPLEGVGPFREKTKHLRMESRDDRCRLFEYGQGDLEKLAAAYPYD
VPDYA*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU24773
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee