Cullin2 (Xenopus)

• Plasmid: pET15b 6HIS TEV Cullin2 M1-S383 Xenopus V340R L344D
• Parent Plasmid: pET15b 6His TEV
• Expressed: 6His-TEV-Cullin 2 M1-S383 V340R L344D
• Genbank: NP_001080037.1
• Tag: 6His
• Species: Xenopus laevis

Sequence of Insert

GGATCCATGTCATTGAAGCCACGGGTAGTGGATTTTGATGAAACATGGAACAAACTTTTAACTACAATCAAAGCTGTTGTCATGCTGGATTATGTTGAAAGAGCAACATGGAATGACCGTTTCTCAGATATTTACGCACTGTGTGTTGCCTATCCAGAGCCTCTTGGAGAAAGATTGTATACAGAAACAAAGATATTTCTGGAAAACCATGTACAGCAGTTGCATACGAGAGTTCTGGATTCAGCAGAGCAAGTACTGGTAATGTACTTCAGATACTGGGAGGAATATAGCAGAGGAGCTGATTACATGGATTGTTTATATAGGTATCTTAACACACAGTACATTAAAAAGAACAAGCTGACAGAAGCAGACCTGCAGTATGGCTATGGTGGAGTAGATATGAATGAACCTCTGATGGAGATAGGAGAATTAGCACTGGATTTGTGGAGGAAATTAATGACAGAGCCTCTCCAAGACACGCTCCTCATAATGCTTCTCCGAGAAATTAAAAGGGATCGATGTGGGGAAGACCCAAATCAGAAAGTAATCCATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTTCCCTTAAAGTTTTATCAAGAAATATTTGAGTCGCCTTTTCTGGCTGAAACAGGGGAGTATTATAAACAAGAAGCTTCAAATTTATTACAAGAGTCAAACTGCTCACAATATATGGAAAAGATTTTAGGTAGATTAAAAGATGAAGAAATCAGATGTCGGAAGTATTTACACCCCAGCTCATATAATAAAGTGATTCACGAATGCCAGCAGAGAATGGTGGCAGATCACTTGCAGTTTCTACATGCGGAATGTCACAATATCATCAGACAAGAAAGGAGAAACGATATGGCCAACATGTATACTCTTCTTCGTGCTGTATCCAGTGGTTTACCTCATATGATTCAGGAACTCCAAAACCATATCCACGATGAAGGCCTTAGAGCTATTAGTAATCTTTCTCAGGAAAACATGCCAACTCAATTTCGGGAGTCAGTTGATGAAGTTCACAGTAAATTTGTTCAGCTTGTCAACTGTGTATTGAATGGTGATCAGCATTTTATGAGTGCACTAGACAAGGCTCTTACTTGTGTGGTGAACTACAGGGAACCAAAATCAtgaGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGENLYFQGHMLGSMSLKPRVVDFDETWNKLLTTIKAVVMLDYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVQQLHTRVLDSAEQVLVMYFRYWEEYSRGADYMDCLYRYLNTQYIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDLWRKLMTEPLQDTLLIMLLREIKRDRCGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQEIFESPFLAETGEYYKQEASNLLQESNCSQYMEKILGRLKDEEIRCRKYLHPSSYNKVIHECQQRMVADHLQFLHAECHNIIRQERRNDMANMYTLLRAVSSGLPHMIQELQNHIHDEGLRAISNLSQENMPTQFRESVDEVHSKFVQLVNCVLNGDQHFMSALDKALTCVVNYREPKS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU26047
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee