FancI (mouse)

• Plasmid: pFastbac1 mouse FancI M1-E1301-6His (codon-optimised)
• Parent Plasmid: pFastbac1
• Expressed: mouse FancI M1-E1301-6His
• Genbank: Protein sequence matches NP_666058.2
• Tag: 6His
• Species: Mouse

Sequence of Insert

GCGGCCGCTGCCACCATGGACCTGAAGATCCTGTCCCTGGCTACCGACAA
GACCACCGACAAGCTGCAAGAGTTCCTGCAGACCCTGAAGGACGACGACC
TGGCTTCCCTGCTGCAGAACCAGGCTGTGAAGGGTCGTGCTGTGGGCACC
CTGCTGCGTGCTGTGCTGAAGGGTTCCCCCTGCTCCGAAGAGGACGGTGC
TCTGCGCCGTTACAAGATCTACTCCTGCTGCATCCAGCTGGTCGAGTCCG
GCGACCTCCAGCAGGATGTGGCTTCCGAGATCATCGGCCTGCTGATGCTC
GAGGTGCACCACTTCCCTGGTCCTCTGCTGGTGGACCTGGCCTCCGATTT
CGTGGGTGCTGTGCGCGAGGACCGTCTGGTCAACGGCAAGTCCCTGGAAC
TGCTGCCCATCATCCTGACCGCTCTGGCTACCAAGAAGGAAGTCCTGGCT
TGCGGCAAGGGCGACCTGAACGGCGAAGAGTACAAGCGTCAGCTGATCGA
CACCCTGTGCTCCGTGCGTTGGCCCCAGCGTTACATGATCCAGCTGACCT
CCGTGTTCAAGGACGTGTGCCTGACCCCCGAAGAGATGAACCTGGTGGTG
GCTAAGGTGCTGACCATGTTCTCCAAGCTGAACCTGCAAGAGATCCCCCC
ACTGGTGTACCAGCTGCTGGTCCTGTCCTCCAAGGGCTCCCGTCGTTCTG
TGCTGGACGGTATCATCGCTTTCTTCCGTGAACTGGACAAGCAGCACCGC
GAGGAACAGTCCTCCGACGAGCTGTCCGAGCTGATCACCGCTCCTGCTGA
CGAACTGTACCACGTCGAGGGCACCGTGATCCTGCACATCGTGTTCGCTA
TCAAGCTGGACTGCGAGCTGGGTCGCGAGCTGCTGAAGCACCTGAAGGCT
GGTCAACAGGGCGACCCCTCCAAGTGCCTGTGCCCCTTCTCTATCGCTCT
GCTGCTGTCCCTGACCCGTATCCAGCGTTTCGAAGAACAGGTGTTCGACC
TGCTCAAGACCTCCGTCGTGAAGTCCTTCAAGGACCTGCAGCTCCTGCAG
GGTTCCAAGTTCCTCCAGACTCTGGTGCCCCAGAGGACCTGCGTGTCCAC
CATGATCCTGGAAGTCGTGCGCAACTCCGTGCACTCCTGGGACCACGTGA
CCCAGGGCCTGATCGAGTTCGGTTTCATCCTGATGGACTCCTACGGTCCC
AAGAAGATCCTCGACGGCAAGGCTGTCGAGATCGGCACCTCCCTGTCCAA
GATGACCAACCAGCACGCCTGCAAGCTGGGTGCTAACATCCTCCTGGAAA
CCTTCAAGATCCACGAGATGATCCGCCAAGAAATCCTGGAACAGGTGCTG
AACCGTGTCGTGACCCGTACCTCCTCCCCTATCAACCACTTCCTGGACCT
GTTCTCCGACATCATTATGTACGCTCCTCTGATCCTCCAGAACTGCTCCA
AAGTGACCGAGACTTTCGACTACCTGACCTTCCTGCCTCTGCAGACCGTG
CAGGGACTGCTGAAGGCCGTGCAGCCCCTGCTGAAGATCTCCATGTCCAT
GCGCGACTCCCTGATCCTGGTGCTGCGCAAGGCTATGTTCGCTTCCCAGC
TGGACGCTCGCAAGTCCGCTGTGGCTGGTTTCCTGCTGCTCCTGAAGAAC
TTCAAGGTGCTGGGTTCCCTGCCATCCTCCCAGTGCACCCAGTCCATCGG
TGTCACCCAAGTGCGCGTGGACGTGCACTCTCGTTACTCCGCTGTCGCTA
ACGAAACCTTCTGCCTGGAAATCATCGACTCCCTCAAGCGTTCCCTGGGC
CAGCAGGCTGACATCCGTCTGATGCTGTACGACGGTTTCTACGACGTGCT
GCGTCGTAACTCTCAGCTGGCTTCCTCCATCATGCAGACTCTGTTCTCCC
AGCTCAAGCAGTTCTACGAGCCCGAGCCCGACCTGCTGCCTCCTCTGAAG
TTGGGAGCTTGCGTGCTGACTCAGGGTTCCCAGATCTTCCTGCAAGAACC
CCTGGACCACCTCCTGTCCTGCATCCAACACTGCCTGGCTTGGTACAAGT
CCCGTGTGGTGCCCCTGCAGCAAGGCGACGAGGGCGAGGAAGAGGAAGAA
GAACTGTACTCCGAGCTGGACGACATGCTGGAATCCATCACCGTGCGTAT
GATCAAGTCCGAGTTGGAGGACTTCGAGTTGGACAAGTCCGCCGACTTCT
CCCAGAACACCAACGTGGGCATCAAGAACAACATCTGCGCTTGCCTGATC
ATGGGCGTGTGCGAGGTGCTGATGGAGTACAACTTCTCCATCTCGAACTT
CAGCAAGTCCAAGTTCGAAGAGATCCTGAGCCTGTTCACTTGCTACAAGA
AGTTCAGCGACATCCTGTCCGAGAAGGCTGGCAAGGGCAAGGCTAAGATG
ACCTCCAAGGTGTCCGACTCTCTGCTGTCTCTCAAGTTCGTGTCTGACCT
GCTGACCGCCCTGTTCCGTGACTCCATCCAGTCCCACGAGGAATCCCTGT
CCGTGCTGAGATCCTCCGGCGAGTTCATGCACTACGCTGTGAACGTGACC
CTCCAGAAGATCCAGCAGCTCATCCGTACCGGTCACGTGTCCGGACCTGA
CGGCCAGAACCCCGACAAGATCTTCCAGAACCTGTGCGACATCACCCGTG
TGCTGCTGTGGCGTTACACCTCCATCCCCACCTCCGTGGAAGAGTCCGGA
AAGAAGGAAAAGGGCAAGTCCATCTCCCTGCTGTGCCTGGAAGGCCTGCA
AAAGACCTTCTCCGTGGTGCTCCAGTTCTACCAACCCAAGGTGCAGCAAT
TCCTGCAGGCTCTGGACGTGATGGGTACTGAAGAGGAAGAGGCTGGCGTC
ACCGTGACCCAGAGGGCTAGCTTCCAGATCCGTCAGTTCCAGCGTTCTCT
GCTCAACCTGCTGTCCAGCGAAGAGGATGACTTCAACTCCAAGGAAGCCC
TGCTGCTGATCGCCGTGCTGTCCACCCTGTCCCGTCTGCTGGAACCTACC
TCCCCACAGTTCGTGCAGATGCTGTCCTGGACTTCCAAGATCTGCAAGGA
ATACTCTCAAGAGGACGCCAGCTTCTGCAAGTCTCTGATGAACCTCTTCT
TCTCCCTCCACGTGCTGTACAAGTCTCCCGTGACCCTGCTGAGGGACCTG
TCCCAGGACATCCACGGTCAACTGGGAGACATCGACCAGGACGTGGAAAT
CGAAAAGACCGACCACTTCGCTGTGGTCAACCTGCGTACCGCTGCTCCCA
CTGTGTGCCTGCTGGTGTTGTCCCAGGCCGAGAAGGTCCTGGAAGAGGTC
GACTGGCTGATCGCTAAGATCAAGGGTTCCGCTAACCAGGAAACCCTGTC
TGACAAAGTGACTCCCGAGGACGCCTCCTCCCAGGCTGTTCCTCCTACCC
TGCTCATCGAGAAGGCCATCGTGATGCAGCTGGGTACTCTGGTCACCTTC
TTCCACGAACTGGTGCAGACTGCTCTGCCCTCCGGTTCCTGCGTGGACAC
CCTCCTGAAGGGCCTGTCTAAGATCTACAGCACCCTGACCGCCTTCGTGA
AGTACTACCTGCAAGTGTGCCAGTCCTCCCGTGGTATCCCCAACACCGTC
GAGAAGCTGGTCAAGCTGTCCGGTTCCCACCTGACCCCTGTGTGCTACTC
CTTCATCTCCTACGTGCAGAACAAGTCCTCTGACGCTCCCAAGTGCAGCG
AAAAGGAAAAGGCTGCTGTCTCCACCACCATGGCTAAGGTCCTGCGCGAG
ACTAAGCCCATCCCCAACCTGGTGTTCGCCATCGAGCAGTACGAGAAGTT
CCTGATCCAGTTGTCCAAGAAGTCCAAAGTCAACCTGATGCAGCACATGA
AGCTGTCCACTTCCCGCGATTTCAAGATCAAGGGCAGCGTGCTGGACATG
GTGCTCCGCGAGGATGAGCATCATCACCACCACCACTAAGGTACC

Amino Acid Sequence

MDLKILSLATDKTTDKLQEFLQTLKDDDLASLLQNQAVKGRAVGTLLRAV
LKGSPCSEEDGALRRYKIYSCCIQLVESGDLQQDVASEIIGLLMLEVHHF
PGPLLVDLASDFVGAVREDRLVNGKSLELLPIILTALATKKEVLACGKGD
LNGEEYKRQLIDTLCSVRWPQRYMIQLTSVFKDVCLTPEEMNLVVAKVLT
MFSKLNLQEIPPLVYQLLVLSSKGSRRSVLDGIIAFFRELDKQHREEQSS
DELSELITAPADELYHVEGTVILHIVFAIKLDCELGRELLKHLKAGQQGD
PSKCLCPFSIALLLSLTRIQRFEEQVFDLLKTSVVKSFKDLQLLQGSKFL
QTLVPQRTCVSTMILEVVRNSVHSWDHVTQGLIEFGFILMDSYGPKKILD
GKAVEIGTSLSKMTNQHACKLGANILLETFKIHEMIRQEILEQVLNRVVT
RTSSPINHFLDLFSDIIMYAPLILQNCSKVTETFDYLTFLPLQTVQGLLK
AVQPLLKISMSMRDSLILVLRKAMFASQLDARKSAVAGFLLLLKNFKVLG
SLPSSQCTQSIGVTQVRVDVHSRYSAVANETFCLEIIDSLKRSLGQQADI
RLMLYDGFYDVLRRNSQLASSIMQTLFSQLKQFYEPEPDLLPPLKLGACV
LTQGSQIFLQEPLDHLLSCIQHCLAWYKSRVVPLQQGDEGEEEEEELYSE
LDDMLESITVRMIKSELEDFELDKSADFSQNTNVGIKNNICACLIMGVCE
VLMEYNFSISNFSKSKFEEILSLFTCYKKFSDILSEKAGKGKAKMTSKVS
DSLLSLKFVSDLLTALFRDSIQSHEESLSVLRSSGEFMHYAVNVTLQKIQ
QLIRTGHVSGPDGQNPDKIFQNLCDITRVLLWRYTSIPTSVEESGKKEKG
KSISLLCLEGLQKTFSVVLQFYQPKVQQFLQALDVMGTEEEEAGVTVTQR
ASFQIRQFQRSLLNLLSSEEDDFNSKEALLLIAVLSTLSRLLEPTSPQFV
QMLSWTSKICKEYSQEDASFCKSLMNLFFSLHVLYKSPVTLLRDLSQDIH
GQLGDIDQDVEIEKTDHFAVVNLRTAAPTVCLLVLSQAEKVLEEVDWLIA
KIKGSANQETLSDKVTPEDASSQAVPPTLLIEKAIVMQLGTLVTFFHELV
QTALPSGSCVDTLLKGLSKIYSTLTAFVKYYLQVCQSSRGIPNTVEKLVK
LSGSHLTPVCYSFISYVQNKSSDAPKCSEKEKAAVSTTMAKVLRETKPIP
NLVFAIEQYEKFLIQLSKKSKVNLMQHMKLSTSRDFKIKGSVLDMVLRED
EHHHHHH*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU50148
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee