SAE2

• Plasmid: pETDuet-1 GST Thrombin SAE1, 6-His Precission SAE 2 C173A
• Parent Plasmid: pETDuet1
• Expressed: 6His-SAE2 C173A
• Genbank: NM_005499.2
• Tag: 6His
• Species: Human

Sequence of Insert

CCATGGGACACCATCACCATCACCATCTGGAAGTTCTGTTCCAGGGGCCA
ATGGCACTGTCGCGGGGGCTGCCCCGGGAGCTGGCTGAGGCGGTGGCCGG
GGGCCGGGTGCTGGTGGTGGGGGCGGGCGGCATCGGCTGCGAGCTCCTCA
AGAATCTCGTGCTCACCGGTTTCTCCCACATCGACCTGATTGATCTGGAT
ACTATTGATGTAAGCAACCTCAACAGACAGTTTTTGTTTCAAAAGAAACA
TGTTGGAAGATCAAAGGCACAGGTTGCCAAGGAAAGTGTACTGCAGTTTT
ACCCGAAAGCTAATATCGTTGCCTACCATGACAGCATCATGAACCCTGAC
TATAATGTGGAATTTTTCCGACAGTTTATACTGGTTATGAATGCTTTAGA
TAACAGAGCTGCCCGAAACCATGTTAATAGAATGTGCCTGGCAGCTGATG
TTCCTCTTATTGAAAGTGGAACAGCTGGGTATCTTGGACAAGTAACTACT
ATCAAAAAGGGTGTGACCGAGTGTTATGAGTGTCATCCTAAGCCGACCCA
GAGAACCTTTCCTGGCgcTACAATTCGTAACACACCTTCAGAACCTATAC
ATTGCATCGTTTGGGCAAAGTACTTGTTCAACCAGTTGTTTGGGGAAGAA
GATGCTGATCAAGAAGTATCTCCTGACAGAGCTGACCCTGAAGCTGCCTG
GGAACCAACGGAAGCCGAAGCCAGAGCTAGAGCATCTAATGAAGATGGTG
ACATTAAACGTATTTCTACTAAGGAATGGGCTAAATCAACTGGATATGAT
CCAGTTAAACTTTTTACCAAGCTTTTTAAAGATGACATCAGGTATCTGTT
GACAATGGACAAACTATGGCGGAAAAGGAAACCTCCAGTTCCGTTGGACT
GGGCTGAAGTACAAAGTCAAGGAGAAGAAACGAATGCATCAGATCAACAG
AATGAACCCCAGTTAGGCCTGAAAGACCAGCAGGTTCTAGATGTAAAGAG
CTATGCACGTCTTTTTTCAAAGAGCATCGAGACTTTGAGAGTTCATTTAG
CAGAAAAGGGGGATGGAGCTGAGCTCATATGGGATAAGGATGACCCATCT
GCAATGGATTTTGTCACCTCTGCTGCAAACCTCAGGATGCATATTTTCAG
TATGAATATGAAGAGTAGATTTGATATCAAATCAATGGCAGGGAACATTA
TTCCTGCTATTGCTACTACTAATGCAGTAATTGCTGGGTTGATAGTATTG
GAAGGATTGAAGATTTTATCAGGAAAAATAGACCAGTGCAGAACAATTTT
TTTGAATAAACAACCAAACCCAAGAAAGAAGCTTCTTGTGCCTTGTGCAC
TGGATCCTCCCAACCCCAATTGTTATGTATGTGCCAGCAAGCCAGAGGTG
ACTGTGCGGCTGAATGTCCATAAAGTGACTGTTCTCACCTTACAAGACAA
GATAGTGAAAGAAAAATTTGCTATGGTAGCACCAGATGTCCAAATTGAAG
ATGGGAAAGGAACAATCCTAATATCTTCCGAAGAGGGAGAGACGGAAGCT
AATAATCACAAGAAGTTGTCAGAATTTGGAATTAGAAATGGCAGCCGGCT
TCAAGCAGATGACTTCCTCCAGGACTATACTTTATTGATCAACATCCTTC
ATAGTGAAGACCTAGGAAAGGACGTTGAATTTGAAGTTGTTGGTGATGCC
CCGGAAAAAGTGGGGCCCAAACAAGCTGAAGATGCTGCCAAAAGCATAAC
CAATGGCAGTGATGATGGAGCTCAGCCCTCCACCTCCACAGCTCAAGAGC
AAGATGACGTTCTCATAGTTGATTCAGATGAAGAAGATTCTTCAAATAAT
GCCGACGTCAGTGAAGAAGAGAGAAGCCGCAAGAGGAAATTAGATGAGAA
AGAGAATCTCAGTGCAAAGAGGTCACGTATAGAACAGAAGGAAGAGCTTG
ATGATGTCATAGCATTAGATTGAGTCGAC

Amino Acid Sequence

MGHHHHHHLEVLFQGPMALSRGLPRELAEAVAGGRVLVVGAGGIGCELLK
NLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVGRSKAQVAKESVLQFY
PKANIVAYHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRMCLAADV
PLIESGTAGYLGQVTTIKKGVTECYECHPKPTQRTFPGATIRNTPSEPIH
CIVWAKYLFNQLFGEEDADQEVSPDRADPEAAWEPTEAEARARASNEDGD
IKRISTKEWAKSTGYDPVKLFTKLFKDDIRYLLTMDKLWRKRKPPVPLDW
AEVQSQGEETNASDQQNEPQLGLKDQQVLDVKSYARLFSKSIETLRVHLA
EKGDGAELIWDKDDPSAMDFVTSAANLRMHIFSMNMKSRFDIKSMAGNII
PAIATTNAVIAGLIVLEGLKILSGKIDQCRTIFLNKQPNPRKKLLVPCAL
DPPNPNCYVCASKPEVTVRLNVHKVTVLTLQDKIVKEKFAMVAPDVQIED
GKGTILISSEEGETEANNHKKLSEFGIRNGSRLQADDFLQDYTLLINILH
SEDLGKDVEFEVVGDAPEKVGPKQAEDAAKSITNGSDDGAQPSTSTAQEQ
DDVLIVDSDEEDSSNNADVSEEERSRKRKLDEKENLSAKRSRIEQKEELD
DVIALD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24687
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee