SAE1

• Synonyms: SUMO-1 Activating Enzyme E1 N Subunit
• Plasmid: pETDuet-1 GST Thrombin SAE1, 6-His Precission SAE 2 C173A
• Parent Plasmid: pETDuet1
• Expressed: GST-SAE1
• Genbank: NM_005500.2
• Tag: GST
• Species: Human

Sequence of Insert

GGTACCATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCA
ACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATT
TGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTG
GGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATT
AACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGT
TGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCG
GTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTT
TGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAA
TGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTA
ACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACAT
GGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAAC
GTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTAT
ATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCA
TCCTCCAAAATCGGATCTGGTTCCGCGTGGATCCATGGTGGAGAAGGAGG
AGGCTGGCGGCGGCATTAGCGAGGAGGAGGCGGCACAGTATGACCGGCAG
ATCCGCCTGTGGGGACTGGAGGCCCAGAAACGGCTGCGGGCCTCTCGGGT
GCTTCTTGTCGGCTTGAAAGGACTTGGGGCTGAAATTGCCAAGAATCTCA
TCTTGGCAGGAGTGAAAGGACTGACCATGCTGGATCACGAACAGGTAACT
CCAGAAGATCCCGGAGCTCAGTTCTTGATTCGTACTGGGTCTGTTGGCCG
AAATAGGGCTGAAGCCTCTTTGGAGCGAGCTCAGAATCTCAACCCCATGG
TGGATGTGAAGGTGGACACTGAGGATATAGAGAAGAAACCAGAGTCATTT
TTCACTCAATTCGATGCTGTGTGTCTGACTTGCTGCTCCAGGGATGTCAT
AGTTAAAGTTGACCAGATCTGTCACAAAAATAGCATCAAGTTCTTTACAG
GAGATGTTTTTGGCTACCATGGATACACATTTGCCAATCTAGGAGAGCAT
GAGTTTGTAGAGGAGAAAACTAAAGTTGCCAAAGTTAGCCAAGGAGTAGA
AGATGGGCCCGACACCAAGAGAGCAAAACTTGATTCTTCTGAGACAACGA
TGGTCAAAAAGAAGGTGGTCTTCTGCCCTGTTAAAGAAGCCCTGGAGGTG
GACTGGAGCAGTGAGAAAGCAAAGGCTGCTCTGAAGCGCACGACCTCCGA
CTACTTTCTCCTTCAAGTGCTCTTAAAGTTCCGTACAGATAAAGGAAGAG
ATCCCAGTTCTGATACATATGAGGAAGATTCTGAGTTGTTGCTCCAGATA
CGAAATGATGTGCTTGACTCACTGGGTATTAGTCCTGACCTGCTTCCTGA
GGACTTTGTCAGGTACTGCTTCTCCGAGATGGCCCCAGTGTGTGCGGTGG
TTGGAGGGATTTTGGCACAGGAAATTGTGAAGGCCCTGTCTCAGCGGGAC
CCTCCTCACAACAACTTCTTCTTCTTCGATGGCATGAAGGGGAATGGGAT
TGTGGAGTGCCTTGGCCCCAAGTGACTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSMVEKEEAGGGISEEEAAQYDRQIR
LWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPE
DPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFT
QFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEF
VEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDW
SSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRN
DVLDSLGISPDLLPEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPP
HNNFFFFDGMKGNGIVECLGPK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24687
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee