Cullin 4B (Xenopus)

• Plasmid: pET28a 6His-TEV Xenopus l. Cullin 4B 1-502 V461R L465D
• Parent Plasmid: pET28a original
• Expressed: 6His-TEV Xenopus l. Cullin 4B 1-502 V461R L465D
• Genbank: NP_001090088.1
• Tag: 6His
• Species: Xenopus laevis

Sequence of Insert

TCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGGTTCTTCCCATCACCATCACCACCACGAAAACCTGTATTTCCAAGGTGGCTCTATGAAAGACCTGGTTGCCTCTCCGAGCGGTCAGAGCAAGCAGGGCCTGAGCAGCCCGAACCCTCCAGCCCAAGAGGTTCGTACCGAAGGTAACAGCACGAGCAAGAAACGTCGCCTGAACATTTCTTCCAGCGATAAAGACGATCTGGATCCGCTGAGCCCGGCTCGTAACTCCAGCTCCAGCAGCAATCATATTCAGAAAAAGCTGCGCTTCGAAGACACCCTGGACTTCCTGGGCCTGGACGTTAAAATGACCGAAGAAAGCTCCTCTTCCTCTAGCTGTTCCTTCTCTTCTACCCCTCAGGCAGTTCGTTCCAAAAATCTGCTGATCTCCCCAGTTGGCGTGGGCCACCATGCGAACGGTCTGACCAAGGCCTCTCCGGTTTCCTCCTTCGCGAACTCTAAGCCGGGCAGCGCTAAAAAACTGGTGATCAAAAACTTCAAAGATAAGCCGAAACTGCCGGAAAACTATACCGACGAAACCTGGCAGAAGCTGAAAGGTACGGTGGAAGCTATCCAGAACTCTACCTCCATCAAATACAACCTGGAGGAACTGTACCAGGCTGTCGAAAATCTGTGTAGCTATAAAATTTCCGCAAACCTGTACAAACAGCTGCGCCAGATCTGCGAAGACCACATCAAAGCGCAGATCCATCAGTTCCGTGAAGATTCTCTGGACTCCGTTCTGTTCCTGAAGAAGATCGACAAATGCTGGAAGGACCATTGTCGTCAAATGATTATGATTCGCAGCATCTTTCTGTTCCTGGACCGTACCTACGTGCTGCAGAACAGCATGCTGCCAAGCATTTGGGACATGGGTCTGGAACTGTTCCGCACCCATATCATCTCTGACCAGAAAGTTCAGAACAAAACTATCGATGGCATCCTGCTGCTGATCGAACGCGAACGTAACGGCGAAGCTATCGACCGCAGCCTGCTGCGTTCCCTGCTGTCCATGCTGAGCGACCTGCAAATTTATCAAGACTCCTTCGAACAGCGTTTCCTGGAGGAAACTAACCGTCTGTACAGCGCTGAAGGCCAGCGCCTGATGCAGGAACGCGAAGTACCAGAATATCTGCACCACGTCAACAAGCGTCTGGAAGAGGAAGCGGATCGTGTTATCACCTATCTGGATCAGTCTACTCAGAAACCGCTGATCGCAACCGTTGAAAAACAGCTGCTGGGCGAACACCTGACCGCAACTCTGCAGAAGGGCCTGAACCATCTGCTGGACGAAAATCGTATCCAGGACCTGTCTCTGCTGTACCAGCTGTTCTCTCGTGTGCGTGGCGGTGTGCAGGTGCTGCTGCAGCACTGGATTGAATACATCAAAGCCTTCGGTAGCACTATTGTTATCAACCCAGAGAAAGATAAAACCATGCGTCAGGAACTGGATGATTTCAAGGACAAAGTAGACCATGTGATCGATGTGTGTTTTCTGAAAAACGAAAAATTCGTTAACGCAATGAAAGAAGCTTTCGAGACCTTTATCAACAAACGCCCTAATTAAGAATTC

Amino Acid Sequence

MGSSHHHHHHENLYFQGGSMKDLVASPSGQSKQGLSSPNPPAQEVRTEGN
STSKKRRLNISSSDKDDLDPLSPARNSSSSSNHIQKKLRFEDTLDFLGLD
VKMTEESSSSSSCSFSSTPQAVRSKNLLISPVGVGHHANGLTKASPVSSF
ANSKPGSAKKLVIKNFKDKPKLPENYTDETWQKLKGTVEAIQNSTSIKYN
LEELYQAVENLCSYKISANLYKQLRQICEDHIKAQIHQFREDSLDSVLFL
KKIDKCWKDHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRTHI
ISDQKVQNKTIDGILLLIERERNGEAIDRSLLRSLLSMLSDLQIYQDSFE
QRFLEETNRLYSAEGQRLMQEREVPEYLHHVNKRLEEEADRVITYLDQST
QKPLIATVEKQLLGEHLTATLQKGLNHLLDENRIQDLSLLYQLFSRVRGG
VQVLLQHWIEYIKAFGSTIVINPEKDKTMRQELDDFKDKVDHVIDVCFLK
NEKFVNAMKEAFETFINKRPN*

• Vector Type: Other
• Antibiotic: Kan
• DU Number: DU49873
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee