Cullin 2 (Xenopus)

• Plasmid: pET28a 6His-TEV Xenopus l. Cullin 2 1-383 V340R L344D
• Parent Plasmid: pET28a original
• Expressed: 6His-TEV Xenopus l. Cullin 2 1-383 V340R L344D
• Genbank: NP_001080037.1
• Tag: 6His
• Species: Xenopus laevis

Sequence of Insert

TCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGGTTCCTCTCACCACCATCATCACCACGAAAACCTGTATTTCCAGGGTGGCTCTATGAGCCTGAAACCGCGTGTTGTGGACTTCGACGAAACGTGGAACAAACTGCTGACGACTATCAAAGCCGTAGTGATGCTGGATTATGTGGAACGTGCTACCTGGAACGACCGCTTCTCCGATATCTATGCGCTGTGCGTTGCATACCCGGAGCCGCTGGGTGAGCGCCTGTATACGGAAACCAAGATTTTCCTGGAGAACCATGTACAGCAGCTGCACACCCGTGTGCTGGACTCTGCGGAACAGGTTCTGGTCATGTACTTCCGTTACTGGGAAGAATACTCCCGTGGTGCCGACTACATGGACTGCCTGTACCGTTACCTGAATACCCAGTACATCAAAAAAAACAAGCTGACGGAAGCGGATCTGCAGTATGGCTACGGTGGTGTTGACATGAACGAACCGCTGATGGAAATCGGTGAACTGGCGCTGGACCTGTGGCGCAAGCTGATGACGGAACCGCTGCAAGATACTCTGCTGATTATGCTGCTGCGTGAAATCAAACGTGATCGCTGTGGCGAAGACCCGAACCAGAAAGTGATCCACGGTGTAATTAATAGCTTTGTTCACGTGGAACAGTACAAAAAAAAATTCCCGCTGAAGTTCTACCAAGAGATCTTTGAATCTCCGTTTCTGGCGGAGACCGGTGAATATTATAAGCAGGAGGCGTCCAATCTGCTGCAGGAATCTAACTGCAGCCAATACATGGAAAAGATCCTGGGTCGCCTGAAAGATGAAGAAATTCGTTGCCGTAAATACCTGCACCCGTCTTCCTATAACAAAGTGATTCACGAATGCCAGCAGCGTATGGTTGCAGATCACCTGCAGTTCCTGCACGCGGAATGCCACAACATCATTCGCCAGGAGCGTCGTAACGACATGGCTAACATGTATACCCTGCTGCGCGCCGTTTCTAGCGGTCTGCCGCACATGATCCAGGAGCTGCAGAACCACATTCATGACGAGGGTCTGCGTGCGATTTCTAACCTGTCTCAGGAAAACATGCCGACTCAGTTCCGTGAAAGCGTGGATGAAGTACACTCTAAATTCGTACAGCTGGTGAACTGTGTGCTGAACGGCGATCAACACTTCATGTCCGCACTGGACAAAGCTCTGACCTGCGTGGTTAACTACCGTGAACCGAAATCCTAAGAATTC

Amino Acid Sequence

MGSSHHHHHHENLYFQGGSMSLKPRVVDFDETWNKLLTTIKAVVMLDYVE
RATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVQQLHTRVLDSAE
QVLVMYFRYWEEYSRGADYMDCLYRYLNTQYIKKNKLTEADLQYGYGGVD
MNEPLMEIGELALDLWRKLMTEPLQDTLLIMLLREIKRDRCGEDPNQKVI
HGVINSFVHVEQYKKKFPLKFYQEIFESPFLAETGEYYKQEASNLLQESN
CSQYMEKILGRLKDEEIRCRKYLHPSSYNKVIHECQQRMVADHLQFLHAE
CHNIIRQERRNDMANMYTLLRAVSSGLPHMIQELQNHIHDEGLRAISNLS
QENMPTQFRESVDEVHSKFVQLVNCVLNGDQHFMSALDKALTCVVNYREP
KS*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU49872
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee